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Research
(Published
online: 23-12-2014)
16. Semen quality parameters, their
inter-relationship and post-washing sperm attributes of Rhode
Island Red roosters -
R. Richard Churchil, P. Ezhil Praveena and
Deepak Sharma
Veterinary World, 7(12): 1117-1122
doi:
10.14202/vetworld.2014.1117-1122
R.
Richard Churchil:
Genome Mapping Laboratory, Central Avian Research Institute,
Izatnagar, Bareilly, Uttar Pradesh, India; Instructional Livestock
Farm Complex, Veterinary College and Research Institute, Tamil
Nadu Veterinary and Animal Sciences
University, Orathanadu, Tamil Nadu, India;
churchil.r@tanuvas.org.in
P.
Ezhil Praveena:
Division of Aquatic Animal Health and Environment, Central
Institute of Brackishwater Aquaculture, # 75, Santhome High Road,
R. A. Puram, Chennai, Tamil Nadu, India;
epraveena@ciba.res.in
Deepak
Sharma: Genome Mapping Laboratory, Central Avian Research
Institute, Izatnagar, Bareilly, Uttar Pradesh, India;
Division of Animal Genetics, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh, India;
ds7758@yahoo.co.in
Received:
20-10-2014, Revised: 19-11-2014 Accepted: 22-11-2014, Published
online: 23-12-2014
Corresponding author:
R. Richard Churchil, e-mail: churchil.r@tanuvas.org.in
Abstract
Aim:
The present experiments were conducted (a) to evaluate the
semen attributes of older Rhode Island Red (RIR) roosters and the
inter-trait relationships, (b) to test sperm washing and storage
duration suitable for gene transfer experiments.
Materials and Methods: The semen characteristics of older RIR
roosters were studied, and Pearson correlation analysis was done
to demonstrate the inter-trait relationships. Progressive motility
and percent live sperms were tested at different post-washing
intervals to identify suitable sperm processing conditions for
gene transfer experiments.
Results: The volume, appearance score, initial motility, sperm
count and percent live and abnormal spermatozoa were 0.38 ml,
3.58, 80.34%, 4.03 × 10 9 sperms/ml, 83.18%
and 4.52% respectively. Positive correlation was observed among
appearance score, motility, live sperm and sperm count. Semen
volume is negatively correlated with all the other characters
except live sperms, whereas, percent abnormal sperms negatively
associated with all the other traits. Significant (p<0.05)
decrease in terms of motility and live sperm was recorded at 60
min post-washing.
Conclusion: The semen attributes of RIR roosters compares well
with the other breeds of chicken. The appearance score can be used
to assess fertility where microscopic evaluation facilities are
limited. The sperm washing protocol tested in the experiment is
suitable for gene transfer experiments.
Keywords: correlation, live sperm,
post-washing motility, Rhode Island Red roosters, semen
attributes.
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