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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online: 24-01-2014)
8. Detection and differentiation of infectious bursal
disease virus from the outbreaks in two layer farms by PCR-RFLP in Jos, Nigeria
- P. D. Luka, B. Yakubu, A. R. Jambol, B. J. Audu, B. B. Dogonyaro
and O. A. Owolodun
Veterinary World, 7(1): 30-33
doi:
10.14202/vetworld.2014.30-33
Abstract
Aim: Characterization of
Infectious bursal disease viruses (IBDV) from the two outbreaks in
Jos Nigeria, using reverse transcriptase polymerase chain
reaction/restriction fragment length polymorphism (RT-PCR/RFLP)
technique.
Materials and Methods: A total of 40 bursa samples were
collected from two outbreaks in November 2011 from two farms of
6-8 weeks old pullets within Jos South Local Government Area, with
mortality between 60 – 74.2 % in commercially reared layer chicken
flocks experiencing signs typical of infectious bursal disease (IBD).
All the samples were found to contain IBDV genome by One Step
RT-PCR using VP2 gene specific primers.
Result: The assay amplified a 743bp fragment from 701-1444
nucleotides. RT-PCR product was further subjected to restriction
digestion using TaqI, MvaI and SacI Restriction enzymes to
differentiate classical from very virulent phenotypes. The RFLP
profile was found similar for all eight isolates with TaqI and
MvaI enzyme but different for SacI. All eight TaqI -positive
Viruses were further found positive for MvaI digestion and yielded
RFLP profile similar to vvIBDV in Europe whereas one isolate was
SacI positive and had a RFLP profile similar to classic IBDV
strains.
Conclusion: The clinical history of high mortality and TaqI
and MvaI restriction enzyme positivity revealed that vvIBDV
strains still exist in Jos, North central Nigeria.
Keywords: infectious bursal disease virus, Nigeria,
outbreak, RT-PCR/RFLP, very virulent
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