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Research
(Published
online: 20-06-2014)
10.
λ -cyhalothrin
induced genotoxicity in freshwater fish Labeo rohita -
P. D. Gadhave, R. S. Brar, H. S. Banga and A. Dhawan
Veterinary World, 7(6): 412-415
doi:
10.14202/vetworld.2014.412-415
P. D. Gadhave: Department of Veterinary Pathology, College
of Veterinary Science, Guru Angad Dev Veterinary and Animal
Sciences University, Ludhiana-141004, India; pdgadhave@gmail.com
R. S. Brar: Department of Veterinary Pathology, College of
Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana-141004, India; rsbrar@email.com
H. S. Banga: Department of Veterinary Pathology, College of
Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana-141004, India; bangahs3@yahoo.com
A. Dhawan: Department of Aquaculture, College of Fisheries
Science, Guru Angad Dev Veterinary and Animal Sciences University,
Ludhiana - 141004, India; dhawanasha@gmail.com
Received: 20-03-2014, Revised: 17-05-2014, Accepted: 21-05-2014,
Published online: 20-06-2014
Corresponding author: P. D. Gadhave, email:
pdgadhave@gmail.com
Abstract
Aim: To study the
genotoxicity of λ-cyhalothrin
in Labeo rohita by determining the induction of micronuclei (MN)
in erythrocytes.
Materials and Methods: The genotoxicity of to
λ-cyhalothrin
was studied using 300 Labeo rohita fingerlings with reference the
guidelines of Organization for Economic Co-operation and
Development (OECD) 204 except the loading density which was higher
than the recommended density 1.0 g/l to reduce the chemical loss.
The disinfection and acclimation for 14 days were done at the site
of experiment. Fish were divided into five groups of 20 fish each.
Group I exposed to 0.27µg/l while Group II exposed to 0.054 µg/l
of λ-cyhalothrin.
There were three control groups, Group III contain only water,
Group IV vehicle control with 0.27µg/l acetone and Group V
positive control with 5 mg/l cyclophosphamide. The experiment was
conducted in triplicate. Water quality parameters like
temperature, pH, total hardness and total alkalinity were measured
on every 15 days. Twenty litres of water from each aquaria was
exchanged daily with water containing respective concentration of
chemical to maintain water quality and chemical concentration. On
15th, 30th and 45th day blood was collected from four fish per
aquaria and pooled to get two blood samples. Three blood smears
were prepared from each blood sample and stained with Giemsa-Wright.
For every blood smear MN frequency per 1000 erythrocytes was
counted. Finally the MN assay was given as average frequency per
group.
Results: The highest frequency of MN was observed on 15th day in
dose dependent manner. However there was time dependent decrease
in frequency of MN on 30th and 45th day.
Conclusions: λ-cyhalothrin
was genotoxic to Labeo rohita and micronuclei assay is suitable
for detecting genotoxicity of
λ-cyhalothrin
in Labeo rohita due to acute exposures.
Keywords: λ-cyhalothrin,
genotoxicity, Labeo rohita, micronuclei
References
|
1. Dutta, H. M. and Dalal, R. (2008) The effect of endosulfan
on the ovary of Bluegill Sunfish: A histopathological study (Lepomis
macrochirus sp). Int. J. Environ. Res. Publ. Health,
2(3):215-224. |
|
|
2. Kaushik, P. and Kaushik, G. (2007). An assessment of
structure and toxicity correlation in organochlorine
pesticides. J. Hazard. Materials, 143:102 -111.
http://dx.doi.org/10.1016/j.jhazmat.2006.08.073
PMid:17011119 |
|
|
3. Ansari, R. A., Rahman, S., Kaur, M., Anjum, S. and
Raisuddin, S. (2011) In vivo cytogenetic and oxidative
stress-inducing effects of cypermethrin in freshwater fish,
Channa punctata Bloch. Ecotoxicol Environ Saf.,74(1):150-156.
http://dx.doi.org/10.1016/j.ecoenv.2010.08.036
PMid:20934752 |
|
|
4. Fetoui, H., Feki, A., Ben Salah, G., Kamoun, H., Fakhfakh,
F. and Gdoura, R. (2013) Exposure to lambda-cyhalothrin, a
synthetic pyrethroid, increases reactive oxygen species
production and induces genotoxicity in rat peripheral blood.
Toxicol Ind Healh. doi: 10.1177/0748233713475516.
http://dx.doi.org/10.1177/0748233713475516 |
|
|
5. Ergene, S., Cavas, T., Celik, A., Koleli, N., Kaya, F., and
Karahan, A. (2007) Monitoring of nuclear abnormalities in
peripheral erythrocytes of three fish species from the Goksu
Delta (Turkey): genotoxic damage in relation to water
pollution. Ecotoxicology, 16:385-391.
http://dx.doi.org/10.1007/s10646-007-0142-4
PMid:17380383 |
|
|
|
6. Food and Agriculture Organization of the United Nations (FAO)
(2012) The State of World Fisheries and Aquaculture. Rome,
2012: 230. |
|
|
7. Ali, D., Nagpure, N. S., Kumar, S., Kumar, R. and Kushwaha,
B. (2008) Genotoxicity assessment of acute exposure of
chlorpyrifos to freshwater fish Channa punctatus (Bloch) using
micronucleus assay and alkaline single-cell gel
electrophoresis. Chemosphere, 71:1823-1831.
http://dx.doi.org/10.1016/j.chemosphere.2008.02.007
PMid:18359502 |
|
|
8. Bahari, I. B., Noor, F. M. and Daud, N. M. (1994)
Micronucleated erythrocytes as an assay to assess actions by
physical and chemical genotoxic agents in Clarias gariepinus.
Mutat Res., 313:1-5.
http://dx.doi.org/10.1016/0165-1161(94)90027-2 |
|
|
9. Grisolia, C. K. and Cordeiro, C. M. T. (2000) Variability
in micronucleus induction with different mutagens applied to
several species of fish. Genet Mol Biol., 23: 235-239.
http://dx.doi.org/10.1590/S1415-47572000000100041 |
|
|
|
10. Ozkan, F., Gunduz, S. G., Berkoz, M. and Hunt, A. O.
(2011) Induction of micronuclei and other nuclear
abnormalities in peripheral erythrocytes of Nile tilapia,
Oreochromis niloticus, following exposure to sublethal cadmium
doses. Turk J Zool., 35(4): 585-592. |
|
|
11. Braunbeck, T., Boettcher, M., Hollert, H., Kosmehl, T.,
Lammer, E., Leist, E., Rudolf, M. and Seitz, N. (2005) Towards
an alternative for the acute fish LC (50) test in chemical
assessment: the fish embryo toxicity test goes multi-species-
an update. Altex., 22(2):87-102.
PMid:15953964 |
|
|
12. Bolognesi, C., Perrone, E., Roggieri, P., Pampanin, D. M.
and Sciutto, A. (2006) Assessment of micronuclei induction in
peripheral erythrocytes of fish exposed to xenobiotics under
controlled conditions. Aquat. Toxicol., 78(Suppl 1): 93-98.
http://dx.doi.org/10.1016/j.aquatox.2006.02.015
PMid:16600396 |
|
|
13. Gokalp, M. F. D. and Guner, U. (2011) Induction of
micronuclei and nuclear abnormalities in erythrocytes of
mosquito fish (Gambusia affinis) following exposure to the
pyrethroid insecticide λ-cyhalothrin. Mutat Res., 726(2):
104-108.
http://dx.doi.org/10.1016/j.mrgentox.2011.05.004
PMid:21620996 |
|
|
|
14. Kumar, A., Sharma, B., Pandey, R. S. (2010) Toxicological
assessment of pyrethroid insecticides with special reference
to cypermethrin and λ-cyhalothrin in freshwater fishes. Int.
J. Biol. Med. Res., 1: 315-325. |
|
|
|
15. Haya, K., Burridge, L. E., Davies, I. M. and Ervik, A.
(2005) A review and assessment of environmental risk of
chemicals used for the treatment of sea lice infestations of
cultured salmon. In: B. Hargrave (Ed.), Handbook of
Environmental Chemistry, Water Pollution, Part M, Vol. 5,
p305-341. |
|
|
16. Moore, A. and Waring, C. P. (2001) The effects of
synthetic pyrethroid pesticide on some aspects of reproduction
in Atlantic salmon (Salmo salar L.) Aquat. Toxicol., 52: 1-12.
http://dx.doi.org/10.1016/S0166-445X(00)00133-8 |
|
|
17. El-Sherif, M. S., Ahmed, M. T., El-Damasoury, M. A. and E
- Nwishy, N. H. K. (2009) Evaluation of diazinon toxicity on
Nile tilapia (Oreochronus niloticus) J. Fish. Aquat Sci., 4:
169-177.
http://dx.doi.org/10.3923/jfas.2009.169.177 |
|
|
|
18. Richterova, Z., Machova, J., Stara, A., Tumova, J.,
Velisek, J., Sevcikova, M. and Svobodova, Z.(2014) Effects of
cyhalothrin-based pesticide on early life stages of common
carp (Cyprinus carpio L.) Biomed. Res. Int. 2014: Article ID
107373. |
|
