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Research
(Published
online: 25-06-2014)
12. Methicillin resistant staphylococci associated with bovine
mastitis and their zoonotic importance - S. Vishnupriya,
P. X. Antony, H. K. Mukhopadhyay, R. M. Pillai, J. Thanislass, V.
M. Vivek Srinivas and R. Sumanth Kumar
Veterinary World, 7(6): 422-427
doi:
10.14202/vetworld.2014.422-427
S. Vishnupriya: Department of Microbiology, Rajiv Gandhi
College of Veterinary and Animal Sciences, Pondicherry University,
Pondicherry - 605 009, India; vishnupriyavet@gmail.com
P. X. Antony: Department of Microbiology, Rajiv Gandhi
College of Veterinary and Animal Sciences, Pondicherry University,
Pondicherry - 605 009, India; pxantony@gmail.com
H. K. Mukhopadhyay: Department of Microbiology, Rajiv
Gandhi College of Veterinary and Animal Sciences, Pondicherry
University, Pondicherry - 605 009, India; hkmparvo@gmail.com
R. M. Pillai: Department of Microbiology, Rajiv Gandhi
College of Veterinary and Animal Sciences, Pondicherry University,
Pondicherry - 605 009, India; drrmadhu@sify.com
J. Thanislass: Department of Veterinary Biochemistry, Rajiv
Gandhi College of Veterinary and Animal Sciences, 605 009, India;
jthanislass@gmail.com
V. M. Vivek Srinivas: Department of Microbiology, Rajiv
Gandhi College of Veterinary and Animal Sciences, Pondicherry
University, Pondicherry - 605 009, India; vivekvet24@gmail.com
R. Sumanth Kumar: Department of Microbiology, Rajiv Gandhi
College of Veterinary and Animal Sciences, Pondicherry University,
Pondicherry - 605 009, India; sumanth.vetco@gmail.com
Corresponding author: V. M. Vivek Srinivas, Present
address: FMD Vaccine Research Laboratory, Indian Veterinary
Research Institute (IVRI), Hebbal, Bangalore-560 024, India. Ph:
+91 97428 91992, email: vivekvet24@gmail.com
Abstract
Aim: The present study was
conducted to determine the zoonotic importance of methicillin
resistant staphylococci associated with bovine mastitis and their
potential role in transmission to animal handlers.
Materials and Methods: A total of 158 milk samples from
bovine mastitis cases and 126 nasal swabs from the animal handlers
were sampled in and around Pondicherry (Southern India). The
Presence of Staphylococcal organism was confirmed by PCR
amplification using the genus specific primers and among the
isolated Staphylococci; methicillin resistance was identified by
genetic amplification of mec A methicillin resistant gene. Then
the amplified gene from the bacteria expressing the mecA gene
(PBP2a) (~2kb fragment) was further sequenced using four sets of
primer pairs and aligned for determining their genetic relatedness
between the sequences. Both phenotypic and genotypic analysis was
carried out for the six MRS isolates (three bovine and three
human) in this study.
Results: Out of 158 mastitis milk samples; 96 and 19 bovine
isolates were found to be positive for Staphylococcal genus
specific PCR and methicillin resistant (mecA) gene PCR,
respectively. Similarly, Out of 126 human nasal swabs, 64 and 13
human isolates were found to be positive for Staphylococcal genus
specific PCR and mec A gene PCR, respectively. Among the 160
staphylococcal isolates (Bovine and Human origin); 51 were
identified as coagulase-positive staphylococci (CPS) and remaining
as coagulase-negative staphylococci (CONS). The results obtained
in this study revealed the presence of many species of
Staphylococci but the predominant species were Staphylococcus
aureus and S. epidermidis. The Sequence analysis of the mec A gene
of human isolates obtained in this study had a maximum identity
(99% -100%) with the bovine isolates.
Conclusion: The phenotypic and genotypic analysis carried
out for the six MRS (Methicillin Resistant Staphylococci) isolates
in this study were indistinguishable and epidemiologically
related, which may indicate the transmission of MRS between bovine
and humans. The occurrence of methicillin resistance among
staphylococci isolated from cases of bovine mastitis is
increasing, necessitating the periodic surveillance for
antimicrobial resistance patterns of Staphylococci in order to
control the spread of MRS.
Keywords: bovine mastitis, coagulase-negative staphylococci
(CONS), methicillin resistant Staphylococcus aureus (MRSA),
sequence analysis.
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