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Research
(Published
online: 26-03-2014)
12. Evaluation of various cultural
enrichment methods for the detection of selected food borne
bacterial pathogens - C. Latha, B. Sunil, V. J. Ajay
Kumar, C. J. Anu and J. Deepa
Veterinary World, 7(3): 172-176
doi:
10.14202/vetworld.2014.172-176
Abstract
Aim: The study was conducted
to evaluate the performance of different enrichment broths such as
Tryptic Soy Yeast extract Broth (TSBYE), Brain Heart Infusion
broth (BHI), Nutrient Broth (NB), Luria Broth (LB) and Peptone
water (PW) for the detection of Listeria monocytogenes,
Yersinia enterocolitica, Staphylococcus aureus and
Salmonella enterica Typhimurium.
Materials and Methods: The bacterial strains were procured
from Institute of Microbial Technology (IMTECH), Chandigarh.
Growth of these food borne pathogens at two different incubation
temperatures (350C and 370C) and three
different incubation periods (12h., 16h. and 18h.) were studied.
Results: The result of the study showed that enrichment in
Tryptic soy broth with yeast extract (TSBYE) and incubation at 370C
for 18h. is superior for the enrichment of all the organisms under
study.
Conclusion: TSBYE can be used
very effectively as universal enrichment broth in comparison with
all other enrichment broths studied for the detection of L.
monocytogenes, Y. enterocolitica, S. aureus and S. enterica
Typhimurium.
Keywords: broth, enrichment, foodborne pathogen, L.
monocytogenes, S. aureus, S. enterica Typhimurium, Y.
enterocolitica.
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