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Research (Published
online: 29-05-2014)
18. Genotyping of the Holstein-Friesian
crossbred cattle for CD18 gene using PCR-RFLP -
A. S. Khade, A. Y. Doiphode, U. D. Umrikar, M. P. Sawane and V. D.
Pawar
Veterinary World, 7(5): 360-362
doi:
10.14202/vetworld.2014.360-362
A. S. Khade: Department
of Animal Genetics and Breeding, Bombay Veterinary College, Parel,
Mumbai-400012, Maharashtra, India.
A. Y. Doiphode: Department
of Animal Genetics and Breeding, Bombay Veterinary College, Parel,
Mumbai-400012, Maharashtra, India.
U. D. Umrikar: Department
of Animal Genetics and Breeding, Bombay Veterinary College, Parel,
Mumbai-400012, Maharashtra, India.
M. P. Sawane: Department
of Animal Genetics and Breeding, Bombay Veterinary College, Parel,
Mumbai-400012, Maharashtra, India.
V. D. Pawar: Department
of Animal Genetics and Breeding, Bombay Veterinary College, Parel,
Mumbai-400012, Maharashtra, India.
Received: 04-03-2014, Revised: 22-04-2014, Accepted: 25-04-2014,
Published online: 29-05-2014
Corresponding author: A.S.
Khade, email: dramolkhade@gmail.com; Tel: +91-8080449956
Abstract
Aim: The present study was
undertaken in Holstein-Friesian (HF) crossbred cattle with the
objective to find out genotype of HF crossbred cattle for Bovine
Leucocyte Adhesion Deficiency (BLAD) by using PCR-RFLP.
Materials and Methods: 50 blood samples were collected from
HF crossbred cattle and subjected to PCR. The amplified PCR
products were digested using Taq I restriction enzyme at 65 oC
overnight. After restriction digestion, the final PCR products
were electrophoresed on 2.5 % agarose gel.
Results: All the 50 animals under present investigation
were found to be normal as the amplified PCR product upon
digestion with Taq I restriction enzyme, revealed two bands of 313
bp and 54 bp for normal animals.
Conclusions: In the present investigation D128G carrier
frequency was found to be 0 %. However, recent reports suggest
that the mutant gene has already been observed in the HF crossbred
cattle population of India, which makes it necessary to screen the
animals to avoid the risk of spreading BLAD in the breeding cattle
population.
Keywords: bovine leucocyte adhesion deficiency, CD18,
genotyping, Holstein-Friesian, PCR-RFLP.
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