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              Open Access  
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, 
distribution and reproduction in any medium, provided the work is properly 
cited. 
 
              
              
              Research 
(Published 
online: 04-05-2014) 
              3. Effect of a specific combination of 
              carvacrol, cinnamaldehyde, and Capsicum oleoresin on the growth 
              performance, carcass quality and gut integrity of broiler chickens
              
              - M. H. H. Awaad, M. Elmenawey and Kawkab A. AhmedVeterinary World, 7(5): 284-290
 
                
              
   
                
                
doi: 
              10.14202/vetworld.2014.284-290 
                
              M. H. H. Awaad: Poultry Diseases Department, Faculty of 
              Veterinary Medicine, Cairo University, Cairo, Egypt.
 
              M. Elmenawey: Animal Production Department, Faculty of 
              Agriculture, Cairo University, Cairo, Egypt. 
              Kawkab A. Ahmed: Animal Production Department, Faculty of 
              Agriculture, Cairo University, Cairo, Egypt. 
              Received: 07-02-2014, Revised: 21-03-2014, Accepted: 27-03-2014, 
              Published online: 04-05-2014
 
              
              Corresponding author: M. 
              H. H. Awaad, email: awaad3@gmail.com, Tel.: + 02-01006882528
 
 
              Abstract 
 
              Aim: The effect of a specific 
              combination (SC) of carvacrol, cinnamaldehyde, and Capsicum 
              oleoresin was investigated on productive performance and immune 
              response in broiler chickens.Materials and Methods: Six hundred one-day-old broiler 
              chickens were randomly allocated into two groups for 5 weeks. The 
              SC was supplemented at 100 ppm of ration (presence or absence).
 Results: Treatment of broiler chickens with the SC improved 
              productive performance variables as compared with the blank 
              control birds. It decreased total mortality, increased final body 
              weight, weight gain, production number and decreased final feed 
              conversion ratio (FCR) (P<0.05). The SC had a positive effect on 
              carcass quality and enhanced HI titer against Newcastle disease 
              (ND) virus vaccine, as compared to their untreated control group 
              (P<0.05). The SC treated birds had higher values of intestinal 
              diameter than the control ones.
 Conclusion: It could be concluded that administration of a 
              specific combination of carvacrol, cinnamaldehyde, and Capsicum 
              oleoresin to broiler chickens improved chicken zootechnical 
              performance response variables, had a potent immuno-modulatory 
              effect (potentiated immune response) and improved gut integrity. 
              Eventually, this combination could be used as a replacement to the 
              controversial feed additives (antibiotic growth promoters).
 Keywords: Capsicum oleoresin, carvacrol, chickens, 
              cinnamaldehyde, gut integrity.
 
 
              References 
 
                
                  | 1. Lee, S.H., Lillehoj, H.S., Jang, S., Kim, D.K., Ionescu, C. 
                  and Bravo, D. (2010) Effect of dietary curcuma, capsicum, and 
                  lentinus on enhancing local immunity against Eimeria 
                  acervulina infection. J. Poult. Sci., 47:89-95. http://dx.doi.org/10.2141/jpsa.009025
 |  
                  |  |  
                  | 2. Lillehoj, H.S., Kim, D.K., Bravo, D.M. and Lee, S.H. 
                  (2010). Effects of dietary plant-derived phytonutrients on the 
                  genome-wide profiles and coccidiosis resistance in the broiler 
                  chickens. International Symposium on Animal Genomics for 
                  Animal Health. Paris, France. 31 May–2 June 2010. |  
                  |  |  
                  | 3. Schuff, S. (2001) Journal says antibiotic use needs 
                  restricting. Feedstuffs, 73(44): 5. |  
                  |  |  
                  | 4. Karimi, A., Yan, F., Coto, C., Park, J.H., Min, Y., Lu, C., 
                  Gidden, J.A., Lay Jr, J.O. and Waldroup, P.W. (2010) Effects 
                  of level and source of oregano leaf in starter diets for 
                  broiler chicks. J. Appl. Poult. Res., 19:137–145. http://dx.doi.org/10.3382/japr.2009-00088
 |  
                  |  |  
                  | 5. Yanhong, L. (2011) Effects of plant extracts on immune 
                  function and disease resistance in pigs. Doctor of Philosophy 
                  in Animal Sciences in the Graduate College of the University 
                  of Illinois at Urbana-Champaign, 2011. |  
                  |  |  
                  | 6. Engster, H. M., Marvil, D. and Stewart-Brown, B. (2002) The 
                  effect of withdrawing growth-promoting antibiotics from 
                  broiler chickens: A long-term commercial industry study. 
                  Poultry Science Association, Inc. 2002. |  
                  |  |  
                  | 7. Norton, R. A. (2000) Learning from Europe's troubling 
                  example. Watt Poultry USA May 2000. |  
                  |  |  
                  | 8. Tokic, V., Lazarevic, M., Sinovic, Z. and Tokic, A. (2007) 
                  The influence of different feed additives to performance and 
                  immune response in broiler chicken. Acta Veterinaria 
                  (Beograd), 57: 217-229. http://dx.doi.org/10.2298/AVB0703217T
 |  
                  |  |  
                  | 9. Yang, Y., Iji, P.A., Kocher, A., Mikkelsen, L.L. and Chotc, 
                  M. (2007) Effects of mannan oligosaccharide on growth 
                  performance, the development of gut microflora, and gut 
                  function of broiler chickens raised on new litter. J. Appl. 
                  Poult. Res., 16: 280-288. http://dx.doi.org/10.1093/japr/16.2.280
 |  
                  |  |  
                  | 10. Awaad, M., Atta, A., Abd El-Ghany, W. A., Elmenawey, M.A., 
                  Ahmed, K., Hassan, A., Nada, A. and Abdelaleem, G. (2011a) 
                  Effect of a specific combination of mannan- oligosaccharides 
                  and ß-glucans extracted from yeast cell wall on the health 
                  status and growth performance of ochratoxicated broiler 
                  chickens. J. Am. Sci., 7: 82-96. |  
                  |  |  
                  | 11. Awaad, M., Atta, A., Elmenawey, M.A., Shalaby, B., 
                  Abdelaleem, G., Madian, K., Ahmed, K., Marzin, D., Benzoni, G. 
                  and Iskander, D. (2011b) Effect of acidifiers on 
                  gastrointestinal tract integrity, zootechnical performance, 
                  and colonization of clostridium perfringens and aerobic 
                  bacteria in broiler chickens. J. Am. Sci., 7:618-628. |  
                  |  |  
                  | 12. Mikulski, D., Zdunczyk, Z., Jankowski, J. and Juskiewicz, 
                  J. (2008) Effects of organic acids or natural plant extracts 
                  added to diets for turkeys on growth performance, 
                  gastrointestinal tract metabolism and carcass characteristics. 
                  J. Anim. Feed Sci., 17: 233-246. |  
                  |  |  
                  | 13. Windisch, W., Schedle, C. and Kroismaryr, A. (2008) Use of 
                  phytogenic products as feed additives for swine and poultry. 
                  J. Anim. Sci., 86 40-148. |  
                  |  |  
                  | 14. Renata S., Lauková, A., Chrastinová, L., Strompfová, V., 
                  Simonová, M. P., Plachá, I., Vasilková, Z., Chrenková, M. and 
                  Faix, S. (2012) Beneficial effect of plant extracts in rabbit 
                  husbandry. Acta Vet. Brno., 81: 245–250. http://dx.doi.org/10.2754/avb201281030245
 |  
                  |  |  
                  | 15. Sarac, N., Ugur, A., Duru, M.E. and Varol, O. (2009). 
                  Antimicrobial activity, antioxidant activity, and chemical 
                  composition of Origanum onites L. and Origanum vulgare L. ssp. 
                  hirtum (Link) ietswaart from Mugla (Turkey). Acta Horticul, 
                  826: 397-404. |  
                  |  |  
                  | 16. Kollanoor-Johny, A., Upadhyay, A., Baskaran, S. A., 
                  Upadhyaya, I., Mooyottu, S., Mishra, N., Darre, M.J., Khan, 
                  M.I., Donoghue, A.M., Donoghue, D. J. and Venkitanarayanan, K. 
                  (2012) Effect of therapeutic supplementation of the plant 
                  compounds trans-cinnamaldehyde and eugenol on Salmonella 
                  enterica serovar enteritidis colonization in market-age 
                  broiler. J. Appl. Poult. Res., 21: 816-822. http://dx.doi.org/10.3382/japr.2012-00540
 |  
                  |  |  
                  | 17. Lee, S.H., Lillehoj, H.S., Jang, S., Lillehoj, E., Min, W. 
                  and Bravo, D. (2013) Dietary supplementation of young broiler 
                  chickens with Capsicum and turmeric oleoresins increases 
                  resistance to necrotic enteritis. Brit. J. Nutr., 9: 1-8. |  
                  |  |  
                  | 18. Sökmen, M., Serkedjieva, J., Daferera, D., Gulluce, M., 
                  Polissiou, M., Tepe, B., Akpulat, H. A., Sahin, F., and Sokmen, 
                  A. (2004) In vitro antioxidant, antimicrobial, and antiviral 
                  activities of the essential oil and various extracts from 
                  herbal parts and callus cultures of Origanum acutidens. J. 
                  Agric. Food Chem., 52:3309-3312. http://dx.doi.org/10.1021/jf049859g
 PMid:15161188
 |  
                  |  |  
                  | 19. Dundar, E., Olgun, E., Isiksoy, S., Kurkcuoglu, M., Baser, 
                  K.H. and Bal, C. (2008) The effects of intra-rectal and intra- 
                  peritoneal application of Origanum onites L. essential oil on 
                  2, 4, 6-trinitrobenzenesulfolic acid-induced colitis in the 
                  rat. Exp. Toxicol. Pathol., 59:399-408. http://dx.doi.org/10.1016/j.etp.2007.11.009
 PMid:18222658
 |  
                  |  |  
                  | 20. Lee, J., Chang, K. and Kim, G. (2009) Composition and 
                  anti- inflammatory activities of Zanthoxylum schinifolium 
                  essential oil: suppression of inducible nitric oxide 
                  synthesis, cyclooxygenase-2, cytokines, and cellular adhesion. 
                  J. Sci. Food Agric., 89:1762-1769. http://dx.doi.org/10.1002/jsfa.3653
 |  
                  |  |  
                  | 21. Lee, K.W., Everts, H. and Beynen, A.C. (2004) Essential 
                  oils in broiler nutrition. Int. J. Poult. Sci., 3:738-752. http://dx.doi.org/10.3923/ijps.2004.738.752
 |  
                  |  |  
                  | 22. Alp M., Midilli, M., Kocabagl, N., Yilmaz, H., Turan, N., 
                  Gargil, A. and Acar, N. (2012) The effects of dietary oregano 
                  essential oil on live performance, carcass yield, serum 
                  immunoglobulin G level, and oocyst count in broilers. J. Appl. 
                  Poult. Res., 21: 630-636. http://dx.doi.org/10.3382/japr.2012-00551
 |  
                  |  |  
                  | 23. Hernandez, F., Madrid, J., Garcia, V., Orengo, J. and 
                  Megias, M.D. (2004) Influence of two plants extracts on 
                  broiler performance, digestibility, and digestive organ size. 
                  Poult. Sci., 83:169-174. http://dx.doi.org/10.1093/ps/83.2.169
 PMid:14979566
 |  
                  |  |  
                  | 24. Timmerman, H., Veldman, A., van den Elsen, E., Rombouts, 
                  F. and Beynen, A. (2006) Mortality and growth performance of 
                  broilers given drinking water supplemented with chicken- 
                  specific probiotics. Poult. Sci., 85:1383–1388. http://dx.doi.org/10.1093/ps/85.8.1383
 PMid:16903468
 |  
                  |  |  
                  | 25. Swayne, D.E., Glisson, J.R., Jackwood, M.W., Pearson, J.E. 
                  and Reed, W.M. (1998) A laboratory manual for the isolation 
                  and identification of avian pathogens. 4th Ed, American 
                  Association of Avian Pathologist Inc., Kennett Square, 
                  Pennsylvania, USA. p156-163. |  
                  |  |  
                  | 26. Bancroft, J.D., Stevens, A. and Turner, D.R. (1996) Theory 
                  and Practice of Histological Techniques. 4th Ed. New York, 
                  Churchill Livingstone. p99-112. |  
                  |  |  
                  | 27. SAS, Institute. (2004) SAS User's Guide, Statistics, 
                  Version 6.12 edition. SAS Institute Inc., Cary, NC.USA. p170. |  
                  |  |  
                  | 28. Bassett, R., (2000) Oreganos positive impact on poultry 
                  production. Wr Poult.-Elsevier, 16:31-34. |  
                  |  |  
                  | 29. Alçiçek, A., Bozkurt, M. and Çabuk, M. (2003) The effects 
                  of an essential oil combination derived from selected herbs 
                  growing wild in turkey on broiler performance. S. Afr. J. Anim. 
                  Sci., 33:89-94. http://dx.doi.org/10.4314/sajas.v33i2.3761
 |  
                  |  |  
                  | 30. Cross, D. E., Acamovic, T., Deans, S. G. and Mcdevitt, R. 
                  M. (2002) The effect of dietary inclusions of herbs and their 
                  volatile oils on the performance of growing chickens. Br. 
                  Poult. Sci. 43:33-35. http://dx.doi.org/10.1080/000716602762388536
 |  
                  |  |  
                  | 31. Bolukbasi, S. C., Erhan, M.K. and Ozkan, A. (2006) Effect 
                  of dietary thyme oil and vitamin E on growth, lipid oxidation, 
                  meat fatty acid composition and serum lipoproteins of 
                  broilers. S. Afr. J. Anim. Sci., 36:189-196. |  
                  |  |  
                  | 32. Botsoglou, N., Florou-Paner, P., Christaki, E., Fletouris, 
                  D.G. and Spais, A.B. (2002) Effect of dietary oregano 
                  essential oil on performance of chickens and on iron induced 
                  lipid oxidation of breast, thigh and abdominal fat tissues. 
                  Br. Poult. Sci., 43:223-230. http://dx.doi.org/10.1080/00071660120121436
 PMid:12047086
 |  
                  |  |  
                  | 33. Botsoglou, N., Christaki, E., Paner, P.F., Giannenas, I., 
                  Papageorgiou, G. and Spais, A. (2004) The effect of a mixture 
                  of herbal essential oils or a-tocopheryl acetate on 
                  performance parameters and oxidation of body lipid in broiler. 
                  S. Afr. J. Anim. Sci., 34:52-61. http://dx.doi.org/10.4314/sajas.v34i1.4039
 |  
                  |  |  
                  | 34. Gill, C. (2001) Safe and sustainable feed ingredients. 
                  Feed Int., 22: 40-45. |  
                  |  |  
                  | 35. Azaz, D., Demirci, F., Satil, F., Kürkcüog, U. and Baser, 
                  K.H. (2002) Antimicrobial activity of some Satureja oils. Z. 
                  Naturforsch., 57:817-821. |  
                  |  |  
                  | 36. Dorman, H. J. D. and Deans, S. G. (2000) Antimicrobial 
                  agent from plants: antimicrobial activity of plant volatile 
                  oils. J. Appl. Microbiol. 88:308-316. http://dx.doi.org/10.1046/j.1365-2672.2000.00969.x
 PMid:10736000
 |  
                  |  |  
                  | 37. Deans, S.G., and Ritchie, G. (1987) Antibacterial 
                  properties of plant essential oils. Int. J. Food Microbiol. 
                  5:165-180. http://dx.doi.org/10.1016/0168-1605(87)90034-1
 |  
                  |  |  
                  | 38. Burt, S.A., Vlielander, R., Haagsman, H.P. and Veldhuizen, 
                  E.J. (2005) Increase in activity of essential oil components 
                  carvacrol and thymol against Escherichia coli O157:H7 by 
                  addition of food stabilizers. J. Food Prot., 68: 919-926. PMid:15895722
 |  
                  |  |  
                  | 39. Veldhuizen, E., Tjeerdsma, J., Zweijtzer, C., Burt, S. and 
                  Haagsman, H. (2006) Structural requirements for the 
                  antimicrobial activity of carvacrol. J Agric Food Chem., 
                  54:1874-879. http://dx.doi.org/10.1021/jf052564y
 PMid:16506847
 |  
                  |  |  
                  | 40. Baser, K.H. (2008) Biological and pharmacological 
                  activities of carvacrol and carvacrol bearing essential oils. 
                  Curr Pharm Des., 14: 106-119. |  
                  |  |  
                  | 41. Burt, S.A., Fledderman, M., Haagsman, H.P., van Knapen, F. 
                  and Veldhuizen, E.J. (2007) Inhibition of Salmonella enterica 
                  serotype Enteritidis on agar and raw chicken by carvacrol 
                  vapors. Int. J. Food Microbiol, 119:346-350. http://dx.doi.org/10.1016/j.ijfoodmicro.2007.05.002
 PMid:17553584
 |  
                  |  |  
                  | 42. Chang, S.T., Chen, P.F. and Chang, S.C. (2001) 
                  Antibacterial activity of leaf essential oils and their 
                  constituents from Cinnamomum osmophloeum. J. Ethnopharmacol, 
                  77:123-127. http://dx.doi.org/10.1016/S0378-8741(01)00273-2
 |  
                  |  |  
                  | 43. Lee, H.S., and Ahn, Y.J. (1998) Growth inhibiting effects 
                  of cinnamomum cassia bark-derived materials on human 
                  intestinal bacteria. J. Agri. Food Chem., 46:8-12. http://dx.doi.org/10.1021/jf970548y
 PMid:10554188
 |  
                  |  |  
                  | 44. McElroy, A.P., Manning, J.G., Jaeger, L.A., Taub, M., 
                  Williams, J.D. and Hargis, B.M. (19940. Effect of prolonged 
                  administration of dietary capsaicin on broiler growth and 
                  Salmonella enteritidis susceptibility. Avian Dis., 38:329- 
                  333. http://dx.doi.org/10.2307/1591958
 PMid:7980284
 |  
                  |  |  
                  | 45. Jamroz, D. and Kamel, C. (2002) Plant extracts on 
                  immunity, health, and performance. J. Anim. Sci., 80:41. |  
                  |  |  
                  | 46. Sarica, S., Ciftci, A., Demir, E., Kilinc, K. and Yildirim, 
                  Y. (2005) Use of antibiotic growth promoter and two herbal 
                  natural feed additives with and without exogenous enzymes in 
                  wheat based broiler diets. S. Afr. Anim. Sci., 35:61-72. |  
                  |  |  
                  | 47. Bravo, D., Utterback, P. and Parsons, C.M. (2011) 
                  Evaluation of a mixture of carvacrol, cinnamaldehyde, and 
                  capsicum oleoresin for improving growth performance and 
                  metabolizable energy in broiler chicks fed corn and soybean 
                  meal. J. Appl. Poult. Res. 20:115–120. http://dx.doi.org/10.3382/japr.2010-00163
 |  
                  |  |  
                  | 48. Bell, D.D. and Weaver, Jr.W.D. (2002) Commercial Chicken 
                  Meat and Egg Production. 5th Ed. Kluwer academic Publishers. 
                  USA. p1109-1128. http://dx.doi.org/10.1007/978-1-4615-0811-3
 |  |