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              Open Access  
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, 
distribution and reproduction in any medium, provided the work is properly 
cited. 
 
              
              
              Research 
(Published 
online: 06-05-2014) 
              4. Subclinical mastitis in machine milked 
              dairy farms in Punjab: prevalence, distribution of bacteria and 
              current antibiogram
              
              
              - Abdul Q. Mir, B. K. Bansal and D. K. GuptaVeterinary World, 7(5): 291-294
 
                
              
   
                
                
doi: 
              10.14202/vetworld.2014.291-294 
                
              Abdul Q. Mir: Department of Veterinary Medicine, Guru Angad 
              Dev Veterinary and Animal Sciences University, Ludhiana- 141 004, 
              Punjab, India.
 
              B. K. Bansal: Department of Veterinary Medicine, Guru Angad 
              Dev Veterinary and Animal Sciences University, Ludhiana- 141 004, 
              Punjab, India. 
              D. K. Gupta: Department of Veterinary Medicine, Guru Angad 
              Dev Veterinary and Animal Sciences University, Ludhiana- 141 004, 
              Punjab, India.
 Received: 21-02-2014, Revised: 24-03-2014, Accepted: 28-03-2014, 
              Published online: 06-05-2014
 
              
              Corresponding author: B. 
              K. Bansal, email: baljinderbansal@rediffmail.com
 
 
              Abstract 
 
              Aim: Mastitis, a complex 
              disease, even at subclinical stage has a major economic impact on 
              the dairy industry. The disease pattern at machine milking which 
              has recently been introduced in Indian system is to be studied. 
              Therefore the present study was conducted to see the prevalence, 
              distribution and sensitivity pattern of bacteria at subclinical 
              level in machine milked dairy farms in Punjab state.Materials and Methods: The study involved 10 machine milked 
              dairy cow herds in Ludhiana, Patiala, Moga, Bathinda and Ferozpur 
              districts of Punjab. A total 218 HF × Sahiwal cross-bred dairy cow 
              in milk were studied. About 872 quarter foremilk samples were 
              collected to observe the prevalence of disease, distribution of 
              udder pathogens and antibiotic sensitivity pattern.
 Results: Prevalence of specific subclinical mastitis was 
              57.80 % and 30.73% on animal and quarter basis respectively. In 
              specific subclinical mastitis Staphylococci (41.04%) were the main 
              organisms while in case of latent infections Corynebacteria 
              (36.81%) were found to be chief isolates. Erythromycin, 
              Enrofloxacin and Gentamicin were found to be most sensitive, and 
              Streptomycin was found to be the least sensitive in vitro 
              antibiotic.
 Conclusion: Staphylococci were the main organisms in 
              specific subclinical mastitis while Corynebacteria were found to 
              be chief isolates in case of latent infections. Erythromycin, 
              Enrofloxacin and Gentamicin were found to be most sensitive, and 
              Streptomycin was found to be the least sensitive in vitro 
              antibiotic.
 Keywords: antibiogram, bacterial distribution, machine 
              milking, prevalence, subclinical mastitis.
 
 
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