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Research
(Published
online: 27-11-2014)
20. Size tunable gold nanoparticle and its
characterization for labeling application in animal health -
P. R. Sahoo and Praveen Singh
Veterinary World, 7(11): 1010-1013
doi:
10.14202/vetworld.2014.1010-1013
P. R.
Sahoo:
Animal Biochemistry Division, Indian Veterinary Research
Institute, Izatnagar, Bareilly, Uttar Pradesh, India;
pravasvet86@gmail.com
Praveen
Singh: Animal Biochemistry Division, Indian Veterinary
Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;
Biophysics
and
Electron Microscopy Section, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh, India;
psingh67@yahoo.com
Received:
28-06-2014, Revised: 08-10-2014, Accepted: 15-10-2014, Published:
27-11-2014
Corresponding author:
P. R. Sahoo, e-mail: pravasvet86@gmail.com
Abstract
Aim:
The aim of the present study was to synthesize different sizes
of gold nanoparticles (GNPs) and their characterization for use as
a label in lateral flow assay particular for the detection of
bluetongue in small ruminants.
Materials and Methods: Size controlled synthesis of GNPs was
done by using different concentration of sodium citrate. In this
study, five different types of GNP were synthesized by using
trisodium citrate (Na 3C6H5O72H2O)
that reduces 20 mM concentration of gold solution (HAuCl4).
These different types of GNPs were characterized in terms of
morphology, size, shape and λmax
by transmission electron microscopy and
ultraviolet-visible spectroscopy respectively.
Results: In the present work, it was found that the size of
GNP mainly depends upon the concentration of sodium citrate. By
use of 0.09375%, 0.1875%, 0.375%, 0.75% and 1.5% of sodium citrate
solution, GNPs were synthesized. In our study, the size of GNP was
found ranging from 25 nm to 230 nm. The size was found large with
less concentration of sodium citrate (i.e. with 0.09735%) and
small with large concentration of sodium citrate (1.5%) and λmax
was found to be 450-530 nm in all size of GNP.
Conclusions: The size of GNPs is mainly dependant on the
concentration of trisodium citrate, gold salt concentration,
optimum pH and temperature. The GNP synthesized by this method has
been used as a label for the development of lateral flow assay
against diagnosis of bluetongue disease in small ruminant.
Keywords: gold nanoparticles, size controlled synthesis,
sodium citrate, tetrachloroauric acid, transmission electron
microscopy.
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