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Research
(Published
online: 30-11-2014)
23.
Detection of extended-spectrum β-lactamases
(blaCTX-M-1
and
blaTEM)
in Escherichia coli, Salmonella spp., and
Klebsiella pneumoniae isolated from poultry in North Eastern
India -
H. Lalzampuia, T. K.
Dutta, Iadarilin Warjri and Rajesh Chandra
Veterinary World, 7(11): 1026-1031
doi:
10.14202/vetworld.2014.1026-1031
H. Lalzampuia:
Department of Veterinary Microbiology, Central Agricultural
University, Selesih, Aizawl, Mizoram, India;
ateahlawndo@gmail.com
T. K.
Dutta:
Department of Veterinary Microbiology, Central Agricultural
University, Selesih, Aizawl, Mizoram, India;
tapandutta@rediffmail.com
Iadarilin Warjri:
Department of Veterinary Microbiology, Central Agricultural
University, Selesih, Aizawl, Mizoram, India;
iadawarjri@gmail.com
Rajesh
Chandra: Department of Veterinary Microbiology, Central
Agricultural University, Selesih, Aizawl, Mizoram, India;
rajeshchandra2k@rediffmail.com
Received:
05-08-2014, Revised: 19-10-2014, Accepted: 27-10-2014, Published
online: 30-11-2014
Corresponding author:
T. K. Dutta, e-mail: tapandutta@rediffmail.com
Abstract
Aim:
The present study was conducted to record the association of
extended spectrum β-lactamases (ESBLs) producing enteric bacteria
with diarrhea of poultry birds in Mizoram, India.
Materials and Methods: Fecal samples were collected from
poultry birds with the history of diarrhea from different parts of
Mizoram. Samples were processed for isolation and identification
of Escherichia coli, Salmonella, and Klebsiella
pneumoniae. All the isolates were subjected to antibiotic
sensitivity assays. Phenotypically, ESBLs production ability was
determined by double discs synergy test (DDST) method. ESBLs
producing isolates were subjected to polymerase chain reaction (PCR)
for detection of ESBLs genes. Plasmids were cured by acridine
orange. Transfer of resistance from donor to recipient strains was
done by in vitro horizontal method.
Results: A total of 134 enteric bacteria was isolated, of
which 102 (76.12%), 21 (15.67%) and 11 (8.21%) were E. coli,
Salmonella spp. and K. pneumoniae, respectively. By
DDST 7 (5.22%) isolates (6 E. coli and 1 K. pneumoniae)
were ESBLs producer. PCR analysis confirmed 5 (3.73%) (4 E.
coli and 1 K. pneumoniae) isolates harboured
blaCTX-M-1
gene
and/or blaTEM
gene. All the isolates were carrying plasmids
ranging between 0.9 kb and ~30 kb. Of the 4 isolates positive for
blaCTX-M-1 and/or
blaTEM,
2 (1.84%) were confirmed for blaCTX-M-1
gene in their plasmid. No
blaTEM
gene was detected from plasmid. The resistance
plasmid could not be transferred to the recipient by in vitro
horizontal gene transfer method.
Conclusion: ESBLs producing enteric bacteria are circulating
in poultry in North Eastern Region of India. As poultry is one of
the most common food animals in this region, these organisms may
enter in human population through them.
Keywords: blaCTX-M-1,
blaTEM,
extended spectrum β-Lactamases,
North East India, poultry.
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