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Research
(Published
online: 13-10-2014)
12.
Effect of uterine immunomodulation on
hematobiochemical parameters in cyclic non-breeding cows -
Saraswat Sahoo, Debendra Narayan Mohanty, Srinibas Das and
Arpita Padhy
Veterinary World, 7(10): 816-820
doi:
10.14202/vetworld.2014.816-820
Saraswat Sahoo:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Orissa
University of Agriculture and Technology, Bhubaneswar, Odisha,
India;
saraswat.vet06@gmail.com
Debendra Narayan Mohanty:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Orissa
University of Agriculture and Technology, Bhubaneswar, Odisha,
India;
mohanty.dn@gmail.com
Srinibas Das:
Department of Teaching Veterinary Clinical Complex, College of
Veterinary Science and Animal Husbandry, Orissa University of
Agriculture and Technology, Bhubaneswar, Odisha, India;
dassrinibas@gmail.com
Arpita Padhy: Department of Veterinary Microbiology, College
of Veterinary Science and Animal Husbandry, Orissa University of
Agriculture and Technology, Bhubaneswar, Odisha, India;
dearlita123@gmail.com
Received: 09-06-2014, Revised: 04-09-2014, Accepted: 08-09-2014,
Published online: 13-10-2014
Corresponding author:
Saraswat Sahoo, e-mail: saraswat.vet06@gmail.com
Abstract
Aim:
To study the effect of uterine immunomodulation on
hematobiochemical parameters and total immunoglobulin
concentration in cyclic non-breeding cows.
Materials and Methods: Twenty-one repeat breeding cows around
Bhubaneswar area were screened by white side test to detect and
treat the endometritis and were assigned to three treatment
protocols with an equal number of seven animals in each group.
Cows in control group were administered with 50 ml of normal
saline while treatment Group I animals were given single
intrauterine infusion of 20 ml of fresh colostrum and treatment
Group II animals received nonpathogenic Escherichia coli in
10 ml sterile saline. The blood samples were collected from all
the experimental animals, and hematobiochemical parameters and
total immunoglobulin concentration were estimated.
Results: A high significant difference (p<0.01) was accounted
in lymphocyte count of E. coli treated group within
different days of sampling. Analysis of variance recorded a highly
significant difference with neutrophil percent in E. coli
lavaged cows. In colostrum treated group monocyte count showed a
significant difference (p<0.01) between 0 and 14th
day of sampling. The analysis of hematocrit values did not show
any significant difference apart from the erythrocyte
sedimentation rate parameter in the colostrum infused group with
the highest significant (p<0.01) variation being observed between
7th and 14th day of sampling. The analysis of aspartate amino
transferase values in the colostrum lavaged cows revealed a
significant difference, but that of alanine amino transferase
values did not show any significant difference. Comparison of
immunoglobulin values for different days in all the treatment
protocol revealed a highly significant (p<0.05) difference within
various days of sampling.
Conclusion: In the present study, the local immunomodulation
by different agents have been highlighted and which indicated
potentiation of uterine immunity by different drug that might
serve as a new direction of treatment to uterine diseases. The
scope of research in the future should be widened by considering a
larger population for validation.
Keywords: colostrum, cyclic non-breeders, hematocrit values,
non-pathogenic Escherichia coli.
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