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Research
(Published
online: 31-10-2014)
26.
Hemato-biochemical and oxidative effect of
fresh juice and ethanolic extract of Ficus tsiela Roxb in
rats - Litty Mathew, N.
Divakaran Nair, N. Vijayan and K. A. Mercey
Veterinary World, 7(10): 890-894
doi:
10.14202/vetworld.2014.890-894
Litty
Mathew: Department
of Pathology, College of Veterinary and Animal Sciences, Mannuthy,
Kerala, India;
drlittymathew@gmail.com
N.
Divakaran Nair:
Department of Pathology, College of Veterinary and Animal
Sciences, Mannuthy, Kerala, India;
divakaran@kvasu.ac.in
N.
Vijayan:
Department of Pathology, College of Veterinary and Animal
Sciences, Mannuthy, Kerala, India;
vijayan@kvasu.ac.in
K. A.
Mercey: Department of Statistics, College of Veterinary and
Animal Sciences, Mannuthy, Kerala, India;
mercey@kvasu.ac.in
Received:
05-07-2014, Revised: 20-09-2014, Accepted: 23-09-2014, Published
online: 31-10-2014
Corresponding author:
Litty Mathew, e-mail: drlittymathew@gmail.com
Abstract
Aim:
The goal was to assess the hemato-biochemical and oxidative
changes of Ficus tsiela Roxb in rats.
Materials and Methods: A total of 48 adult female Sprague-Dawley
rats weighing 200-250 g were divided into six groups with eight
rats in each group. Group 1 received no treatment served as a
control group. Group 2 and 3 animals were orally ingested with
fresh juice from 5 g of leaves and 10 g of leaves, respectively.
Group 4, 5 and 6 animals were orally administered with the extract
at doses of 750, 1000 and 1500 mg/kg, respectively. The experiment
was continued for a period of 21 days. Biochemical parameters
including aspartate aminotransferase (AST), alkaline phosphatase
(ALP), creatinine kinase (CK), creatinine and blood glucose and
hematological parameters such as hemoglobin (Hb), packed cell
volume (PCV), total leukocyte count (TLC) and differential
leucocyte count (DLC) were determined on 0, 7, 14, and 21 day of
treatment. Lipid peroxides and reduced glutathione values were
determined in liver at day 21.
Results: Hb, PCV, TLC and DLC showed no significant variations
in treated groups compared with control and ALP, AST, CK and
creatinine values showed a significant increase in serum of the
higher dose groups. There was no variation in the level of blood
sugar. There was an increase in the values of lipid peroxides and
reduction in the values of reduced glutathione in the liver
homogenate which indicated oxidative damage.
Conclusion: The fresh juice and ethanolic extract at higher
doses had no effect on hematological values. However, biochemical
parameters affected adversely. Both fresh juice and extract caused
oxidative damage to liver.
Keywords: biochemical, Ficus tsiela, phytotoxicity.
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