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Research
(Published
online: 13-09-2014)
10.
Prevalence and antibiotic
resistance pattern of Campylobacter species in foods of
animal origin - Pallavi and Ashok
Kumar
Veterinary World, 7(9): 681-684
doi:
10.14202/vetworld.2014.681-684
Pallavi:
Division of Veterinary Public Health, Indian Veterinary Research
Institute, Izatnagar, Bareilly, Uttar Pradesh, India;
upadhyayapallavi.31@gmail.com
Ashok
Kumar: Division of Veterinary Public Health, Indian Veterinary
Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;
ashokakt@rediffmail.com
Received:
01-06-2014, Revised: 23-07-2014, Accepted: 31-07-2014, Published
online: 13-09-2014
Corresponding author: Pallavi,
email: upadhyayapallavi.31@gmail.com
Abstract
Aim:
The aim was to determine the prevalence and evaluation of
antibiotic resistance pattern and minimum inhibitory concentration
(MIC) of Campylobacter species isolated from foods of
animal origin.
Materials and Methods: A total of 280 samples (comprising 150
chicken meat, 50 chevon and 80 milk) were collected from retail
meat markets, slaughter houses and dairy farms and analyzed for
isolation of Campylobacter species. A total of 29 isolates
comprising 23 Campylobacter jejuni and 6 Campylobacter
coli were recovered, characterized biochemically and confirmed
by polymerase chain reaction. These isolates were then tested for
antibiotic resistance pattern through disc diffusion method, and
MIC was assessed by MIC strips. The antibiotic resistance
assessment was performed against 8 antibiotics viz. ampicillin,
co-trimoxazole, erythromycin, levofloxacin, gentamicin,
ciprofloxacin, ceftriaxone, and norfloxacin.
Results: The prevalence of Campylobacter spp. in
chicken meat, chevon and milk samples were observed 17.33%, 6% and
0%, respectively. All the isolates were resistant to co-trimoxazole
but sensitive to erythromycin. All the isolates showed different
resistance pattern for the rest of the antibiotics. MIC results
revealed that all the isolates were within prescribed
concentrations for sensitivity for the antibiotics tested.
Conclusions: The foods of animal origin are source of
Campylobacter infections to human beings. Thus, the
development of antibiotic-resistant strains emphasizes the
requirement of better surveillance and monitoring of the foods of
animal origin and the use of antimicrobials in veterinary and
human medicine require careful regulation.
Keywords: antibiotic resistance,
Campylobacter, minimum inhibitory concentration, poultry.
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