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R esearch
(Published online:
07-04-2015)
1.
Bronchoalveolar lavage is an ideal tool in evaluation of local
immune response of pigs vaccinated with Pasteurella multocida
bacterin vaccine
-
Shiney
George, Nagendra Nath Barman, Anjan Jyoti Nath and Bhupen Sarma
Veterinary World, 8(4): 438-442
doi:
10.14202/vetworld.2015.438-442
Shiney George: North
Eastern Regional Disease Diagnostic Laboratory, Government of
Assam, Khanapara, Guwahati - 781 022, Assam, India;
shineygeorge0@gmail.com
Nagendra Nath Barman:
Department of Microbiology, College of Veterinary Science,
Khanapara, Guwahati - 781 022, Assam, India;
nnbarman@gmail.com
Anjan Jyoti Nath:
Pasteur Institute of India, Coonoor - 643 013, Nilgiris, Tamil
Nadu, India;
drnath76@gmail.com
Bhupen Sarma:
Department of Surgery and Radiology, College of Veterinary
Science, Assam Agricultural University, Khanapara, Guwahati - 781
022, Assam, India;
bhupensarma59@gmail.com
Received:
21-11-2014,
Revised:
23-02-2015, Accepted:
02-03-2015, Published online:
07-04-2015
Corresponding author:
Nagendra Nath Barman, e-mail: nnbarman@gmail.com
Citation:
George
S, Barman NN, Nath AJ, Sarma B. Bronchoalveolar lavage is an ideal
tool in evaluation of local immune response of pigs vaccinated
with Pasteurella multocida bacterin vaccine, Veterinary
World 8(4): 438-442.
Abstract
Aim: The aim was to study
the bronchoalveolar lavage (BAL) technique in evaluating the local
immune response of pig immunized with Pasteurella multocida
bacterin vaccine.
Materials and Methods: Weaned piglets were immunized with
formalin-inactivated P52 strain of
P. multocida bacterin and evaluated for pulmonary
immune response in BAL fluid. BAL was performed before vaccination
and at different post vaccination days. The BAL fluid was assayed
using enzyme-linked immunosorbent assay to study the development
of
P. multocida specific antibody isotypes and also
evaluated for different cell populations using standard protocol.
Results:
The average recovery
percentage of BAL fluid varies from 58.33 to 61.33 in vaccinated
and control group of piglets. The BAL fluid of vaccinated pigs
showed increase in antibody titer up to 60th
days post vaccination
(8.98±0.33), IgG being the predominant isotype reached maximum
titer of 6.12±0.20 on 45th
days post vaccination,
followed by IgM and a meager concentration of IgA could be
detected. An increased concentration of the lymphocyte population
and induction of plasma cells was detected in the BAL fluid of
vaccinated pigs.
Conclusion: Though intranasal vaccination with
P. multocida plain
bacterin vaccine could not provoke a strong immune response, but
is promising as lymphocyte population was increased and plasma
cells were detected. BAL can be performed repeatedly up to 3/4
months of age in pigs to study pulmonary immune response without
affecting their health.
Keywords:
bronchoalveolar lavage,
cell types, isotype specific enzyme linked immunosorbent assay,
P. multocida, pig,
pulmonary immune response.
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