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              Open Access  
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
 
 
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distribution and reproduction in any medium, provided the work is properly 
cited. 
 
              
              
              Research 
              
              
(Published online: 
              19-04-2015) 
              
              15.
              
              Hemato-biochemical and hormonal profiles in 
              post-partum water buffaloes (Bubalus bubalis) -
              Sunil 
              Kumar, A. K. Balhara, Rajesh Kumar, Naresh Kumar, Lukumoni 
              Buragohain, Daoharu Baro, R. K. Sharma, S. K. Phulia and Inderjeet 
              Singh 
              
              Veterinary World, 8(4): 512-517   
              
   
                
                
doi: 
              10.14202/vetworld.2015.512-517     Sunil 
              Kumar: 
              
              Division of Animal Reproduction, Indian Veterinary Research 
              Institute, Izatnagar, Uttar Pradesh, India; sunil.vet89@gmail.com A. K. 
              Balhara: 
              
              Division of Animal Physiology and Reproduction, Central Institute 
              for Research on Buffalo, Hisar, Haryana, India; balharaak@gmail.com Rajesh 
              Kumar: 
              
              Division of Animal Physiology and Reproduction, Central Institute 
              for Research on Buffalo, Hisar, Haryana, India; drrajesh7.vet@gmail.com Naresh 
              Kumar: 
              Department of Biochemistry, College of Basic Sciences and 
              Humanities, CCS Haryana Agricultural University, Haryana, India; 
              soninaresh1234@gmail.com 
              Lukumoni Buragohain: 
              
              Department of Animal Biotechnology, College of Veterinary and 
              Animal Science, Lala Lajpat Rai University of Veterinary and 
              Animal Sciences, Haryana, India; lukumoni.buragohain54@gmail.com Daoharu 
              Baro: 
              Department of Animal Biotechnology, College of Veterinary and 
              Animal Science, Lala Lajpat Rai University of Veterinary and 
              Animal Sciences, Haryana, India; dao.haru@yahoo.com R. K. 
              Sharma: 
              
              Division of Animal Physiology and Reproduction, Central Institute 
              for Research on Buffalo, Hisar, Haryana, India; rksharma@scientist.com S. K. 
              Phulia: 
              
              Division of Animal Physiology and Reproduction, Central Institute 
              for Research on Buffalo, Hisar, Haryana, India; sphulia@gmail.com 
              Inderjeet Singh: 
              
              Division of Animal Physiology and Reproduction, Central Institute 
              for Research on Buffalo, Hisar, Haryana, India; inderjeet.dr@gmail.com   
              Received: 14-01-2015, Revised: 16-03-2015, Accepted: 20-03-2015, 
              Published online: 19-04-2015   
              
              
              Corresponding author: 
              
              Sunil Kumar, e-mail: sunil.vet89@gmail.com 
 
              Citation:Kumar S, Balhara AK, Kumar R, Kumar N, Buragohain L, Baro D, 
              Sharma RK, Phulia SK, Singh I (2015) Hemato-biochemical and 
              hormonal profiles in post-partum water buffaloes (Bubalus 
              bubalis), Veterinary World 8(4); 512-517. 
 
              Abstract 
 Aim:
              The objective of the present study was to compare serum as 
              well as follicular fluid (FF) biochemical and hormonal profiles 
              along with hematological parameters in postpartum estrus, anestrus, 
              and cystic buffaloes. 
              Materials and Methods: Postpartum buffaloes were selected in 
              three different groups (within 40-60 days of parturition at 
              estrus-Group-I, postpartum >90 days at anestrum-Group-II, and 
              postpartum cystic buffaloes in Group III). The animals selected 
              were examined for follicular wave dynamics by routine trans-rectal 
              ultrasonography and FF was collected by transvaginal 
              ultrasound-guided ovum pick up technique. All hematological and 
              biochemical parameters were analyzed by automatic analyzers while 
              hormonal profiles analyzed by commercially available ELISA kits. 
              Results: In the present investigation, estrum and anestrum 
              animal differ significantly in hemoglobin levels. Serum estradiol 
              differs significantly in estrus and anestrus while no significant 
              difference in progesterone concentration was noted among all three 
              stages. The results of our study suggest that significant higher 
              increase in total protein (TP), calcium and glucose values in 
              estrum while urea, aspartate aminotransferase, alanine 
              aminotransferase, lactate dehydrogenase significantly higher in 
              anestrum animals. 
              Conclusion: The conclusion of the present study is that TP and 
              albumin, calcium, urea, glucose affects oocyte development and 
              quality. 
              Keywords: biochemical, buffalo, follicular 
              fluid, hematology, ovum pick up. 
 
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