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R esearch
(Published online:
21-04-2015)
17.
Utility of a rapid immunochromatographic
strip test in detecting canine parvovirus infection compared with
polymerase chain reaction - Sundaran S. Tinky, R.
Ambily, Sreeja R. Nair and Mangattumuruppel Mini
Veterinary World, 8(4): 523-526
doi:
10.14202/vetworld.2015.523-526
Sundaran S. Tinky:
Department of Veterinary Microbiology, College of Veterinary and
Animal Sciences, Kerala Veterinary and Animal Sciences
University, Mannuthy, Thrissur, Kerala, India; ss4tinky@gmail.com
R.
Ambily:
Department of Veterinary Microbiology, College of Veterinary and
Animal Sciences, Kerala Veterinary and Animal Sciences University,
Mannuthy, Thrissur, Kerala, India;
ambilysd@gmail.com
Sreeja R. Nair:
Department of Veterinary Microbiology, College of Veterinary and
Animal Sciences, Kerala Veterinary and Animal Sciences
University, Mannuthy, Thrissur, Kerala, India; drsreejarnair@gmail.com
Mangattumuruppel Mini: Department of Veterinary Microbiology,
College of Veterinary and Animal Sciences, Kerala Veterinary and
Animal Sciences University, Mannuthy, Thrissur, Kerala, India;
mini@kvasu.ac.in
Received: 18-12-2014, Revised: 19-03-2015, Accepted: 26-03-2015,
Published online: 21-04-2015
Corresponding author:
R. Ambily, e-mail: ambilysd@gmail.com
Citation:
Tinky SS, Ambily R, Nair SR, Mini M. (2015)
Utility of a rapid immunochromatographic strip test in detecting
canine parvovirus infection compared with polymerase chain
reaction, Veterinary World 8(4);
523-526.
Abstract
Aim:
The present study was undertaken to detect the presence of
canine parvovirus (CPV) in fecal samples of diarrheic dogs by
conventional polymerase chain reaction (PCR) and
immunochromatographic (IC) strip test and to compare the
diagnostic potential of these tests.
Materials and Methods: A total of 50 fecal samples collected
from diarrheic dogs suspected for CPV infection were subjected to
PCR using CPV-555 primer amplifying the gene coding for the VP1
protein. These samples were also tested by IC strip test using a
commercial rapid Ag test kit. The results were statistically
analyzed using McNemar test.
Results: A total of 22 samples (44%) were
detected as positive by PCR, which yielded a specific amplicon of
583 bp. In IC strip test, 18 (36%) samples were found to be
positive. The sensitivity of the test as compared to PCR was found
to be 72.22% and specificity was 92.86%. Positive predictive value
and negative predictive value of IC strip test was found to be
88.89% and 81.25%, respectively. Statistical analysis of the
results of PCR and IC assay using McNemar test revealed no
significant difference (p>0.05).
Conclusion: The IC strip test could be employed as a rapid
field level diagnostic tool for the diagnosis of canine parvoviral
diarrhea.
Keywords: canine parvoviral diarrhea,
immunochromatographic strip test, polymerase chain reaction.
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