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              Open Access  
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cited. 
 
              
              
              Research 
              
              
(Published online: 
              
              
              29-04-2015) 
              
              23.
              
              Effect 
              of varying levels of formaldehyde treatment of mustard oil cake on 
              rumen fermentation, digestibility in wheat straw based total mixed 
              diets 
              in 
              vitro 
              - 
              
              Mahima, Vinod Kumar, S. K. Tomar, Debashis Roy and Muneendra Kumar 
              
              Veterinary World, 8(4): 551-555   
              
   
                
                
doi: 
              10.14202/vetworld.2015.551-555 
                
              Mahima: 
              
              Department of Animal Nutrition, College of Veterinary Science and 
              Animal Husbandry, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu 
              Chikitsa Vigyan Vishwavidyalay Evum Go-Anusandhan Sansthan (DUVASU), 
              Mathura, Uttar Pradesh, India; 
              
              chahal_mahima@rediffmail.com 
              Vinod Kumar: 
              
              Department of Animal Nutrition, College of Veterinary Science and 
              Animal Husbandry, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu 
              Chikitsa Vigyan Vishwavidyalay Evum Go-Anusandhan Sansthan (DUVASU), 
              Mathura, Uttar Pradesh, India; 
              
              vinodsidhu@rediffmail.com 
              S. K. Tomar: 
              Dairy 
              Cattle Nutrition Division, National Dairy Research Institute, 
              Karnal, Haryana, India: 
              
              santoshktomar@gmail.com 
              Debashis Roy:
              
              
              Department of Animal Nutrition, College of Veterinary Science and 
              Animal Husbandry, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu 
              Chikitsa Vigyan Vishwavidyalay Evum Go-Anusandhan Sansthan (DUVASU), 
              Mathura, Uttar Pradesh, India; 
              
              debashish2k4@gmail.com 
              Muneendra Kumar: Department of Animal 
              Nutrition, College of Veterinary Science and Animal Husbandry, 
              Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan 
              Vishwavidyalay Evum Go-Anusandhan Sansthan (DUVASU), Mathura, 
              Uttar Pradesh, India; 
              
              muneedra82@gmail.com    Received: 
              28-11-2014, Revised: 11-03-2015, Accepted: 18-03-2015, Published 
              online: 29-04-2015    
              
              
              Corresponding author:Mahima, 
              e-mail: chahal_mahima@rediffmail.com 
 
              Citation:Mahima, Kumar V, Tomar SK, Roy D, Kumar M 
              (2015) Effect of varying levels of formaldehyde treatment of 
              mustard oil cake on rumen fermentation, digestibility in wheat 
              straw based total mixed diets in vitro, Veterinary World 
              8(4); 551-555. 
 
              Abstract 
 
              Aim: The aim of the current 
              study was to protect the protein in mustard cake by different 
              levels of formaldehyde treatment with a view to optimize the level 
              of formaldehyde.  
              Materials and Methods: Different levels of formaldehyde 
              treatment (0, 1, 1.5 and 2% of crude protein) containing 
              concentrate and roughages diet in 40:60 ratio were tested for 
              their effect on nutrients digestibility, in vitro ammonia 
              release, in vitro gas production and change 
              in protein fractions. Non-significant (p≤0.05) effect on pH, 
              microbial biomass, 
              partitioning factor, total gas production (TGP), TGP per g dry 
              matter and TGP per g digestible dry matter (ml/g) was observed in 
              almost all the treatments. 
              Results: Total volatile fatty acids at 2% formaldehyde 
              treatment level of mustard cake was lower (p<0.05) as compared to 
              other groups, while in vitro dry matter digestibility and
              in vitro organic matter digestibility were reported to be 
              low in 1% formaldehyde treated group.  
              Conclusion: On a holistic view, it could be considered that 
              formaldehyde treatment at 1.5% level was optimal for protection of 
              mustard oil cake protein.  
              Keywords: ammonia release, in vitro gas fermentation 
              technique, mustard cake, ruminal fluid, ruminal fermentation, 
              protected protein, protein fractions, volatile fatty acids.  
 
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