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              Open Access  
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cited. 
 
              
              
              Research 
              
              
(Published online: 
              09-04-2015) 
              
              4.
              
              Effect 
              of supplementation of lysine producing microbes vis-a-vis source 
              and level of dietary protein on performance and egg quality 
              characteristics of post-peak layers 
              - 
              G. U. 
              Manju, B. S. V. Reddy, Gideon Gloridoss, T. M. Prabhu, K. S. 
              Giridhar and N. Suma 
              
              Veterinary World, 8(4): 453-460   
              
   
                
                
doi: 
              10.14202/vetworld.2015.453-460     
              G. U. Manju: 
              Department of Animal Nutrition, Livestock 
              Research and Information Center, Karnataka Veterinary and 
              Fisheries Science University, Nagamangala, Mandya, Karnataka, 
              India; drmanjucric313@gmail.com 
              B. S. V. Reddy:
              Department of Animal Nutrition 
              Veterinary College, Karnataka Veterinary and Fisheries Science 
              University Hebbal, Bangalore, Karnataka, India; mansricha@gmail.com 
              Gideon Gloridoss:
              Department of Animal Nutrition, 
              Veterinary College, Karnataka Veterinary and Fisheries Science 
              University, Hebbal, Bangalore, India; gideongloridoss@gmail.com 
              T. M. Prabhu: 
              Department of Animal Nutrition, 
              Veterinary College, Karnataka Veterinary and Fisheries Science 
              University, Hebbal, Bangalore, India; prabhutmann@gmail.com 
              K. S. Giridhar:
              Department of Animal Nutrition, 
              Veterinary College, Karnataka Veterinary and Fisheries Science 
              University, Shivamogga, Karnataka, India; giridharksdr@gmail.com 
              N. Suma: 
              Department of Animal Nutrition, Veterinary College, Karnataka 
              Veterinary and Fisheries Science University, Hassan, Karnataka, 
              India; sumavet@gmail.com    Received: 
              04-11-2014, Revised: 01-03-2015, Accepted: 09-03-2015, Published 
              online: 09-04-2015    
              
              
              Corresponding author: 
              
              G. U. Manju, e-mail: 
              drmanjucric313@gmail.com 
 
              Citation:Manju GU, Reddy BSV, Gloridoss G, Prabhu TM, Giridhar KS, Suma N 
              (2015) Effect of supplementation of lysine producing microbes vis-a-vis 
              source and level of dietary protein on performance and egg quality 
              characteristics of post peak layers, 
              
              Veterinary World 8(4): 453-460. 
 
              Abstract 
 
              Aim: The aim was to study 
              the effect of supplementation of lysine producing microbes (LPM) 
              as an in vivo source of lysine on performance and egg 
              quality characters of post-peak layers.  
              Materials and Methods: BIS (1992) specified diets (except 
              crude protein [CP] and lysine) were prepared using either soybean 
              meal (SBM) or groundnut extractions (GNE) or sunflower extractions 
              (SFE) with 16 and 15% CP resulting in six control diets. Further, 
              each control diet was fortified with either synthetic lysine or 
              LPM to meet BIS (1992) specified lysine requirement resulting in 
              the set of 12 test diets. Each of the eighteen diets was offered 
              to quadruplets groups of 4 post-peak (52 weeks) commercial laying 
              hens in each. The trial lasted for 119 days.  Result:
              The results revealed that the feed consumption and body weight 
              changes and Roche yolk color and yolk index were significantly (p 
              ≤ 0.05) different among different treatments. However, egg 
              production, feed efficiency, egg weight, egg shape index, Haugh 
              unit score, albumen index and shell thickness, and net returns 
              remained non-significant (p ≤ 0.05) among different treatments. 
              Among main factors, protein level (16% and 15% CP) made a 
              significant (p ≤ 0.05) difference in egg production (79.6 and 
              75.1%) and feed efficiency (2.64 and 2.81 kg feed/kg egg mass, 
              respectively). Among protein source GNE- and SFE-based diet fed 
              groups showed significantly (p < 0.0%) higher feed consumption and 
              body weight gain than SBM based diets fed birds. Yolk color (7.0, 
              7.3 and 7.3, respectively) and yolk index (0.40, 0.38 and 0.43, 
              respectively) were significantly (p ≤ 0.05) different from the 
              protein sources. CP level and Protein source interaction effects 
              showed significant differences in albumen index and Haugh unit 
              score.  
              Conclusion: Optimum level of protein (16% CP) and GNE as a 
              source of protein tended to be superior in increasing the 
              performance and egg characteristics of post-peak layers and 
              supplementation of lysine in either synthetic or LPM form found to 
              be beneficial in optimizing their performance.  
              Keywords: egg characteristics, lysine, lysine producing 
              microbes, post peak layers, performance.  
 
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