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R esearch
(Published online:
12-04-2015)
8.
Prevalence of antibiotic resistant Staphylococcus aureus
from raw milk samples collected from the local vendors in the
region of Tirupathi, India
-
Sudhanthirakodi Sudhanthiramani, Chinta Siva Swetha and Sukumar
Bharathy
Veterinary World, 8(4): 478-481
doi:
10.14202/vetworld.2015.478-481
Sudhanthirakodi
Sudhanthiramani: Department of
Veterinary Public Health and Epidemiology, College of Veterinary
Science, Sri Venkateshwara Veterinary University Campus, Tirupati,
Andhra Pradesh - 517 502, India;
sudhanthiramvet@gmail.com
Chinta Siva Swetha:
Department of Veterinary Public
Health and Epidemiology, College of Veterinary Science, Sri
Venkateshwara Veterinary University Campus, Tirupati, Andhra
Pradesh - 517 502, India;
drswetha.vet@gmail.com
Sukumar Bharathy:
Department of Veterinary Public
Health and Epidemiology, College of Veterinary Science, Sri
Venkateshwara Veterinary University Campus, Tirupati, Andhra
Pradesh - 517 502, India;
bharathy.vet@gmail.com
Received: 29-01-2015, Revised: 25-02-2015, Accepted: 05-03-2015,
Published online: 12-04-2015
Corresponding author:
Sudhanthirakodi Sudhanthiramani, e-mail: sudhanthiramvet@gmail.com
Citation:
Sudhanthiramani S, Swetha CS, Bharathy S (2015) Prevalence of
antibiotic-resistant Staphylococcus aureus from raw milk
samples collected from the local vendors in the region of
Tirupathi, India, Veterinary World 8(4): 478-481.
Abstract
Aim: The study was carried
out with the aim to identify the suitability of the milk for
consumer use with special reference to Staphylococcus aureus
from milk samples collected from various local vendors and
determine the antibiotic susceptibility pattern of those positive
isolates.
Materials and Methods: A total of 110 milk samples were
collected from the local milk vendors in and around Tirupathi
region of India. All the samples were enriched with buffered
peptone water in 9:1 ratio and the then inoculated on baird parker
agar medium with added 2% egg yolk tellurite emulsion as selective
medium for S.aureus and confirmed with mannitol salt agar,
Gram’s staining and biochemical tests. The typical cultural
characters with coagulase-positive samples were taken as positive
samples the positive samples were tested for antibiotic
susceptibility with 10 different antibiotics by employing disc
diffusion method.
Results: Prevalence of coagulase-positive S. aureus was
39.09% (43/110) from the milk samples. The antibiotic
susceptibility test of positive isolates showed high resistant
toward penicillin G 37/43 (86.04%) and ampicillin 32/43 (74.42%),
and also showed resistant to methicillin 6/43 (13.95%),
cephalothin 6/43 (13.95%), tetracycline 6/43 (13.95%),
ciprofloxacin 4/43 (9.30%), enrofloxacin 3/43 (6.97%), cefoxitin
2/43 (4.65%), gentamicin 2/43 (4.65%), and co-trimoxazole 2/43
(4.65%). Many individual isolates showed resistant against two or
more antibiotics in our study.
Conclusion: The above study results show that the milk samples
collected from local vendor having S. aureus, which can
induce disease condition as well as antibiotic resistant to the
humans particularly young children and old age peoples by means of
consumption of raw milk and its products. This is the public
health issue, which needs to be solved by educating the local
vendors regarding health problems related to unhygienic milk
supply and make the awareness among the consumers about this
hazards and preventive measures.
Keywords: antibiotic resistant, local milk vendor, milk,
Staphylococcus aureus.
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