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R esearch
(Published online:
11-12-2015)
3. Effect of cholesterol supplementation on cryosurvival
of goat spermatozoa
- Sunita Behera, Hiron M. Harshan, K. Lekshmi Bhai and K.
N. Aravinda Ghosh
Veterinary World, 8(12): 1386-1391
doi:
10.14202/vetworld.2015.1386-1391
Sunita
Behera:
Department of Veterinary Gynaecology and Obstetrics, Veterinary
College, Bengaluru, Karnataka, India;
sunita5656@gmail.com
Hiron
M. Harshan:
Department of Animal Reproduction Gynaecology and Obstetrics,
College of Veterinary and Animal Sciences, Kerala Veterinary and
Animal Sciences University, Pookode, Kerala, India;
hiron@rediffmail.com
K.
Lekshmi Bhai:
Department of Animal Reproduction Gynaecology and Obstetrics,
College of Veterinary and Animal Sciences, Kerala Veterinary and
Animal Sciences University, Pookode, Kerala, India;
bhai04vet@gmail.com
K. N.
Aravinda Ghosh: Department of Animal Reproduction,
Gynaecology and Obstetrics,
College of Veterinary and Animal Sciences, Kerala
Veterinary and Animal Sciences University, Mannuthy, Kerala,
India;
ghosh@kvasu.ac.in
Received: 07-06-2015, Revised: 24-10-2015, Accepted: 31-10-2015,
Published: 11-12-2015
Corresponding author:
Sunita Behera, e-mail: sunita5656@gmail.com
Citation:
Behera S, Harshan HM, Bhai KL, Ghosh KNA (2015) Effect
of cholesterol supplementation on cryosurvival of goat
spermatozoa,
Veterinary World 8(12):
1386-1391.
Abstract
Aim:
Sperm membrane cholesterol
influences cryodamage during cryopreservation. The present study
was carried out to evaluate the effect of varying cholesterol
levels in Tris based extenders on the freezability of sexually
healthy Malabari buck semen.
Materials and Methods:
A total of 48 ejaculates
from two adults healthy sexually healthy Malabari bucks were
utilized for the study. The collected and pooled ejaculates were
divided into four groups with Group I serving as Control - I,
Group II and III were treated with 1 mg and 2 mg of
cholesterol-loaded-cyclodextrin (CLC)/120 × 106
spermatozoa,
respectively, and Group IV treated with 1 mg methyl-β-cyclodextrin
(MβCD) served as Control - II. Manual freezing was carried out
to cryopreserve the treated and control spermatozoa.
Results:
Treatment of semen samples with
CLC resulted in improved maintenance of sperm motility at
pre-freeze and post-thaw stages of cryopreservation without
affecting hypo-osmotic swelling response. Treatment of semen
with 1 mg of CLC/120 × 106
spermatozoa was observed
to be better than treatment with 2 mg of CLC/120 × 106
spermatozoa. In general,
MβCD treatment was found to result in significantly lower sperm
characteristics than those of Control - I and CLC treatment at
pre-feeze and post-thaw stages and when incubated up to 4 h.
Conclusion:
Cholesterol treatment of
sexually healthy Malabari buck semen was found to hold promise
for improving cryopreser-vability of spermatozoa.
Keywords:
buck, cholesterol, cryopreservation, functional membrane
integrity, motility, spermatozoa.
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