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R esearch
(Published online:
11-12-2015)
4.
Evaluation of
antidiarrheal activity of ethanolic extract of
Holarrhena
antidysenterica
seeds in rats -
Dushyant
Kumar Sharma, Vinod Kumar Gupta, Surendra Kumar, Vivek Joshi,
Ravi Shankar Kumar Mandal,
A. G. Bhanu Prakash and Mamta Singh
Veterinary World, 8(12): 1392-1395
doi:
10.14202/vetworld.2015.1392-1395
Dushyant Kumar Sharma:
Division of Medicine, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh - 243 122, India;
dr.dushsharma@gmail.com
Vinod
Kumar Gupta:
Division of Medicine, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh - 243 122, India;
vinodgupta1288@gmail.com
Surendra Kumar:
Division of Medicine, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh - 243 122, India;
mailmesbansal@gmail.com
Vivek
Joshi:
Division of Medicine, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh - 243 122, India;
joshignet@gmail.com
Ravi
Shankar Kumar Mandal:
Division of Medicine, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh - 243 122, India;
dr.ravi0911@gmail.com
A. G. Bhanu Prakash:
Division of Medicine, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh - 243 122, India;
vetbhanu@gmail.com
Mamta
Singh: Division of Medicine, Indian Veterinary Research
Institute, Izatnagar, Bareilly, Uttar Pradesh - 243 122, India;
mamta2947@gmail.com
Received: 01-06-2015, Revised: 25-10-2015, Accepted: 31-10-2015,
Published online: 11-12-2015
Corresponding author:
Dushyant Kumar Sharma, e-mail: dr.dushsharma@gmail.com
Citation:
Sharma DK,
Gupta VK, Kumar S, Joshi V, Mandal RSK, Prakash AGB, Singh M
(2015) Evaluation of antidiarrheal activity of ethanolic extract
of
Holarrhena antidysenterica
seeds
in rats,
Veterinary World 8(12):
1392-1395.
Abstract
Aim:
The present study was conducted
to evaluate the antidiarrheal effect of ethanolic extract of
Holarrhena
antidysenterica
(Family - Apocynaceae) seeds against induced diarrhea in Wistar
albino rats.
Materials and Methods:
The extract was evaluated
for castor oil and
Escherichia coli
induced diarrhea. Extract was given at 100, 200, 400 mg/kg body
wt. orally in both protocols. Standard antidiarrheal Loperamide
was used at 5 mg/kg body wt. orally in castor oil induced
protocol, while standard antibiotic Gentamicin at 8 mg/kg body
wt. intraperitoneally was used in
E. coli
induced diarrhea. In castor oil
induced protocol, the percentage inhibition of defecation was
calculated for each group, whereas in
E. coli
induced protocol, change in
fecal consistency, and body weight was recorded for each
individual rat for 3 days.
Results:
The severity of castor oil
induced diarrhea was reduced significantly (p<0.05) with
H. antidysenterica
seeds extract at 200 and 400
mg/kg body wt. which showed equivalent effectiveness like that
of Loperamide treated groups. Similarly in
E. coli
induced diarrhea protocol, the
mean change in body weight was significantly (p<0.05) higher in
positive control, whereas no significant variation was observed
in negative control, Gentamicin treated and
H. antidysenterica
treated group at 200 mg/kg and
400 mg/kg body wt., respectively.
Conclusion:
The study concluded that
ethanolic extract of
H. antidysenterica
seeds effectively controlled diarrhea and decreased the severity
of clinical signs of castor oil and
E. coli
induced diarrhea in Wistar rats.
Keywords:
antidiarrheal,
Escherichia
coli, Holarrhena antidysenterica.
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