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R esearch
(Published
online:
17-12-2015)
6. Detection of genes mediating beta-lactamase
production in isolates of enterobacteria recovered from wild
pets in Saudi Arabia -
Sabry A. Hassan and Mohammed Y. Shobrak
Veterinary World, 8(12): 1400-1404
doi:
10.14202/vetworld.2015.1400-1404
Sabry
A. Hassan:
Department of Biology, Faculty of Science, Taif University 888,
Taif, Saudi Arabia; Department of Microbiology, Faculty of
Veterinary Medicine, South Valley University 83523, Qena, Egypt;
sabryh@lycos.com
Mohamed Y. Shobrak:
Department of Biology, Faculty of Science, Taif University 888,
Taif, Saudi Arabia;
mshobrak@gmail.com
Received: 24-08-2015, Revised: 19-10-2015, Accepted: 25-10-2015,
Published online: 17-12-2015
Corresponding author:
Sabry A. Hassan, e-mail: sabryh@lycos.com
Citation: Hassan SA, Shobrak MY (2015) Detection of genes
mediating beta-lactamase production in isolates of
enterobacteria recovered from wild pets in Saudi Arabia,
Veterinary World 8(12): 1400-1404.
Abstract
Aim:
To determine the genetic basis and types of beta-lactamase
encountered among enterobacterial isolates of wild pets from the
animal exhibit.
Materials and Methods: A total of 17 beta-lactamase-producing
enterobacteria recovered from fecal samples of wild pet animals
were analyzed for a selected beta-lactamase gene by polymerase
chain reaction.
Results: Molecular analysis identified one or more β-lactamase-encoding
genes in 14 enterobacterial isolates as a single or gene
combination. The most frequent extended-spectrum β-lactamases
types were TEM and CTX-M, and the
most common AmpC enzymes were CMY-2 and DHA types.
Conclusions: The study is the first in Saudi Arabia, have
established the presence of β-lactamase-encoding genes in the
fecal isolates of wild pets.
Keywords: animal exhibit, extended-spectrum β-lactamases/AmpC
beta-lactamase, fecal samples, polymerase chain reaction, Saudi
Arabia.
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