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Research
(Published
online: 30-01-2015)
23.
Virulence genes detection of Salmonella
serovars isolated from pork and slaughterhouse environment in
Ahmedabad, Gujarat -
J. H. Chaudhary, J. B.
Nayak, M. N. Brahmbhatt and P. P. Makwana
Veterinary World, 8(1): 121-124
doi:
10.14202/vetworld.2015.121-124
J. H.
Chaudhary:
Department of Veterinary Public Health, College of Veterinary
Science and Animal Husbandry, AAU, Anand - 388 001, Gujarat,
India;
jitvph007@gmail.com
J. B.
Nayak:
Department of Veterinary Public Health, College of Veterinary
Science and Animal Husbandry, AAU, Anand - 388 001, Gujarat,
India;
jbn_anand@yahoo.com
M. N.
Brahmbhatt:
Department of Veterinary Public Health, College of Veterinary
Science and Animal Husbandry, AAU, Anand - 388 001, Gujarat,
India;
mnbrahmbhatt2003@yahoo.com
P. P.
Makwana: Department of Veterinary Public Health, College of
Veterinary Science and Animal Husbandry, AAU, Anand - 388 001,
Gujarat, India;
paresh.makwana1989@gmail.com
Received:
05-11-2014, Revised: 18-12-2014, Accepted: 18-12-2014, Published
online: 30-01-2014
Corresponding author:
J. H. Chaudhary, e-mail: jitvph007@gmail.com
Abstract
Aim:
The aim was to detect virulence gene associated with the
Salmonella serovars isolated from pork and Slaughterhouse
environment.
Materials and Methods: Salmonella isolates (n=37) used
in this study were isolated from 270 pork and slaughter house
environmental samples collected from the Ahmedabad Municipal
Corporation Slaughter House, Ahmedabad, Gujarat, India.
Salmonella serovars were isolated and identified as per BAM
USFDA method and serotyped at National Salmonella and
Escherichia Centre, Central Research Institute, Kasauli
(Himachal Pradesh, India). Polymerase chain reaction technique was
used for detection of five genes, namely invA, spvR,
spvC, fimA and stn among different serovars
of Salmonella.
Results: Out of a total of 270 samples, 37 (13.70%)
Salmonella were isolated with two serovars, namely Enteritidis
and Typhimurium. All Salmonella serovars produced 284 bp
invA gene, 84 bp fimA and 260 bp amplicon for
enterotoxin (stn) gene whereas 30 isolates possessed 310 bp
spvR gene, but no isolate possessed spvC gene.
Conclusion: Presence of invA, fimA and stn
gene in all isolates shows that they are the specific targets
for Salmonella identification and are capable of producing
gastroenteric illness to humans, whereas 20 Typhimurium serovars
and 10 Enteritidis serovars can able to produce systemic
infection.
Keywords: pork, Salmonella, slaughterhouse environment,
virulence genes
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