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R esearch
(Published online:
26-07-2015)
16.
Prevalence, molecular characterization of
Staphylococcus aureus isolated from cheese and in vitro
antibacterial activity of silver nanoparticles against such
strains -
Karima G. Abdel Hameed
and Mona A. El-Zamkan
Veterinary World, 8(7): 908-912
doi:
10.14202/vetworld.2015.908-912
Karima
G. Abdel Hameed:
Department of Food Hygiene and Control, Faculty of Veterinary
Medicine, South Valley University, Qena, Egypt;
karima_galal2004@yahoo.com
Mona A.
El-Zamkan: Department of Food Hygiene and Control, Faculty of
Veterinary Medicine, South Valley University, Qena, Egypt;
Moon_zam@outlook.sa
Received:
08-05-2015, Revised: 18-06-2015, Accepted: 26-06-2015, Published
online: 26-07-2015
Corresponding author:
Karima G. Abdel Hameed, e-mail: karima_galal2004@yahoo.com
Citation:
Abdel Hameed KG, El-Zamkan
MA (2015) Prevalence, molecular characterization of
Staphylococcus aureus isolated from cheese and in vitro
antibacterial activity of silver nanoparticles against such
strains, Veterinary World 8(7):908-912.
Abstract
Aim:
The aim was to investigate cheese samples for the prevalence
of Staphylococcus aureus, evaluate multiplex polymerase
chain reaction (PCR) methods for S. aureus identification,
as well as to determine the antibacterial activity of silver
nanoparticles against such strains.
Materials and Methods: Total of 100 random locally
manufactured cheese samples were collected from Qena dairy
markets, Egypt, and examined conventionally for the prevalence of
S. aureus then, confirmation of these isolates were done
using multiplex PCR. The antibacterial activity of silver
nanoparticles against such isolates was also checked.
Results: Lower prevalence of S. aureus in Damietta
cheese (54%) than in Kareish cheese (62%) was recorded. As well
lower frequency distribution for both S. aureus (36%) and
CNS (8%) was also reported for Damietta cheese. Using of multiplex
PCR method for S. aureus identification have been confirmed
all 58 S. aureus stains that were identified conventionally
by detection of two PCR products on agarose gel: The 791 bp and
the 638 bp. The correlation coefficient between conventional and
multiplex PCR method was 0.91 and was significant at p ≤0.001.
Regarding antibacterial activity of silver nanoparticles using
disk diffusion method on Baird Parker agar it was found that
inhibition zone of silver nanoparticles against S. aureus,
was 19.2±0.91 mm and it was higher than that produced by
gentamicin (400 units/ml) 15.2±0.89 mm.
Conclusions: The present study illustrated the higher
prevalence of S. aureus in cheese samples that may
constitute a public health hazard to consumers. According to the
results, it can be concluded that silver nanoparticles can be used
as an effective antibacterial against S. aureus. Thereby,
there is a need for an appropriate study for using silver
nanoparticles in cleaning and disinfection of equipment and in
food packaging.
Keywords: antibacterial activity, multiplex
polymerase chain reaction, Staphylococcus aureus, silver
nanoparticles.
References
1. EL-Kholy, A.M., EL-Shinawy, S.H., Meshref, A.M. and Korany,
A.M. (2014) Microbiological quality of domiati cheese and the
influence of probiotics on the behavior of Staphylococcus
aureus and Eescherichia coli O157: H7 in domiati cheese. J.
Food Safety, 34(4): 396-406.
http://dx.doi.org/10.1111/jfs.12157 |
|
|
|
2. Mohammed, A.S. (2010) Sanitary evaluation of some dairy
products in qena markets, M.V.Sc. Thesis, Faculty of
Veterinary Medicine South Valley University. |
|
|
3. Yan, X., Wang, B., Tao, X., Hu, Q., Cui, Z., Zhang, J.,
Lin, Y., You, Y., Shi, X. and Hajo G. (2012) Characterization
of Staphylococcus aureus strains associated with food
poisoning in shenzhen, China. Appl. Environ. Microb., 78(18):
6637-6642.
http://dx.doi.org/10.1128/AEM.01165-12
PMid:22798367 PMCid:PMC3426705 |
|
|
4. William, J.M., Jon, S.B., Karen, B., Noroyono, W., Neelum,
O. and Mark, S.S. (2001) Multiplex PCR protocol for the
diagnosis of Staphylococcal infection. J. Clin. Microbiol.,
39(9): 3332-3338.
http://dx.doi.org/10.1128/JCM.39.9.3332-3338.2001
PMCid:PMC88340 |
|
|
5. Yeo, S.Y., Lee, H.J. and Jeong, S.H. (2003) Preparation of
nanocomposite fibers for permanent antibacterial effect. J.
Mater. Sci., 38: 2143-2147.
http://dx.doi.org/10.1023/A:1023767828656 |
|
|
6. Azlin-Hasim, S., Cruz-Romero, M.C., Ghoshal, T., Morris,
M.A., Cummins, E. and Kerry, J.P. (2014) Application of silver
nanodots for potential use in antimicrobial packaging
applications. Innov. Food Sci. Emerg., 27: 136-143.
http://dx.doi.org/10.1016/j.ifset.2014.10.012 |
|
|
|
7. Finegold, S.H. and Martin, W.J. (1982) Diagnostic
Microbiology, Bailly and Scott. 6th ed. Mosby Co., St., Louis,
Toronto, London. |
|
|
|
8. Bennett, R.W. and Lancette, G.A. (1995) Staphyloccus
aureus. In: Food and Drug Administration Bacteriological
Analytical Manual. 8th ed., Ch. 12. AOAC International,
Gaithersburg, MD. p12.01-12.05. |
|
|
|
9. Hameid, A.K.G., Sender, G., Prusak, B., Ryniewicz, Z.
(2004) Multiplex PCR protocol for the diagnosis of
Staphylococcus aureus udder infections of Polish Black and
White cows. Anim. Sci. Pap. Rep., 22(4): 679-685. |
|
|
10. Lee, K.J., Park, S.H., Govarthanan, M., Hwang, P., Seo,
Y.S., Cho, M., Lee, W.H., Lee, J.Y., Kannan, S.K. and Oh, B.T.
(2013) Synthesis of silver nanoparticles broad using cow milk
and their antifungal activity against phytopathogens. Mater.
Lett., 105: 128-131.
http://dx.doi.org/10.1016/j.matlet.2013.04.076 |
|
|
|
11. Clinical and Laboratory Standards Institute (CLSI). (2002)
Performance Standards for Antimicrobial Disk and Dilution
Susceptibility Tests for Bacterial Isolated from Animals;
Approved Standard, M31-A2. 4th ed. CLSI, Wayne, PA. |
|
|
12. Cheruiyot, K.R., Olila, D. and Katerega, J. (2009)
In-vitro antibacterial activity of selected medicinal plants
from Longisa region of Bomet District, Kenya. Afr. Health
Sci., 9: S42-S46.
PMid:20589160 PMCid:PMC2890996 |
|
|
|
13. Frazier, W.C. and Westhoff, D.C. (1988) Food Microbiology.
4th ed. Mc-Graw Hill Publ. Co. Ltd., New York. |
|
|
|
14. Saad, A.M. (2010) Study on microorganisms of public health
hazards in locally manufactured cheese at Aswan city. M.V.Sc.
Thesis, Faculty of Veterinary Medicine South Valley
University. |
|
|
15. Jay, A.M. (1996) Modern Food Microbiology. 5th ed. Chapman
and Hall Inc., New York, USA.
http://dx.doi.org/10.1007/978-1-4615-7473-6 |
|
|
16. Mcdevitt, D., Francois, P., Vaudaux, P. and Foster, T.J.
(1994) Molecular characterisation of the clumping factor
(fibrinogen receptor) of Staphylococcus aureus. Mol.
Microbiol., 11(2): 237-248.
http://dx.doi.org/10.1111/j.1365-2958.1994.tb00304.x |
|
|
|
17. Smeltzer, M.S., Gillaspy, A.F., Pratt, F.L., Thames, M.D.
and Landolo, J.J. (1997) Prevalence and chromosomal map
location of Staphylococcus aureus adhesion genes. J. Genet.,
196: 249-259. |
|
|
18. Stutz, K., Stephan, R. and Tasara, T. (2011) SpA, ClfA,
and FnbA genetic variations lead to Staphaurex test-negative
phenotypes in bovine mastitis Staphylococcus aureus isolates.
J Clin Microbiol., 49(2): 638-646.
http://dx.doi.org/10.1128/JCM.01148-10
PMid:21147952 PMCid:PMC3043514 |
|
|
19. Kelvin, K., Vincent, C., Jasper, F., Amy, C., Sandy, C.,
Flora, H., Shirly, O., Susanna, K., Kwok, Y. and Patrick, Y.
(2011) Molecular characterization of a catalase - Negative
Staphylococcus aureus subsp. aureus strain collected from a
patient with mitral valve endocarditis and pericarditis
revealed a novel nonsense mutation in the kat Agene. J. Clin.
Microbiol., 49(9): 3398-3402.
http://dx.doi.org/10.1128/JCM.00849-11
PMid:21715584 PMCid:PMC3165592 |
|
|
20. Da Silva, E.R., Sigueira, A.P., Martins, J.C.D., Ferreria,
W.P.B. and Silva, N. (2005) Hemolysin production by
Staphylococcus aureus species isolated from mastitic goat milk
in Brazilian dairy herds. Small Rumin. Res., 56: 271-275.
http://dx.doi.org/10.1016/j.smallrumres.2004.04.011 |
|
|
21. Shrivastava, S., Bera, T., Roy, A., Singh, G.,
Ramachandrarao, P. and Dash, D. (2007) Characterization of
enhanced antibacterial effects of novel silver nanoparticles.
Nanotechnology, 18: 225103, 1-6.
http://dx.doi.org/10.1088/0957-4484/18/22/225103 |
|
|
|
22. Hameed, A.K.G. and El-Malt, M.L. (2009) Public health
hazard of Staphylococcus aureus isolated from raw milk and ice
cream in Qena governorate. Assiut Vet. Med. J., 55(121):
191-200. |
|
|
|
23. Dehkordi, S.H., Fatemeh, H. and Azizollah, E.K. (2011) An
in vitro evaluation of antibacterial effect of silver
nanoparticles on Staphylococcus aureus isolated from bovine
subclinical mastitis, Afr. J. Biotechnol., 10(52):
10795-10797. |
|
|
|
24. Kazemi, J., Malahat, A., Habib, D., Saei, M. and Adib, H.
(2014) Antibacterial effect of silver nanoparticles along with
protein synthesis-inhibiting antibiotics on Staphylococcus
aureus isolated from cattle mastitis. Biol. J. Micro., 8:
15-22. |
|
|
25. Seil, J.T. and Webster, T.J. (2012) Antimicrobial
applications of nanotechnology: Methods and literature. Int.
J. Nanomed., 7: 2767-2781.
PMid:22745541 PMCid:PMC3383293 |
|
|
26. Appendini, P. and Hotchkiss, J.H. (2002) Review of
antimicrobial food packaging. Innov. Food Sci. Emerg., 3:
113-126.
http://dx.doi.org/10.1016/S1466-8564(02)00012-7 |
|
|
27. Zarei, M., Jamnejad, A. and Khajehali, E. (2014)
Antibacterial effect of silver nanoparticles against four
foodborne pathogens. Jundishapur J. Microbiol., 7(1): 8720.
http://dx.doi.org/10.5812/jjm.8720 |
|
