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R esearch
(Published online:
06-06-2015)
1.
Isolation, identification, and characterization of Listeria
spp. from various animal origin foods -
Deepti N. Nayak, C. V.
Savalia, I. H. Kalyani, Rajeev Kumar and D. P. Kshirsagar
Veterinary World, 8(6): 695-701
doi:
10.14202/vetworld.2015.695-701
Deepti
N. Nayak:
Department of Veterinary Public Health and Epidemiology, Vanbandhu
College of Veterinary Science and Animal
Husbandry,
Navsari Agricultural University, Navsari - 396 450, Gujarat,
India; drdeeptin@rediffmail.com
C. V.
Savalia:
Department of Veterinary Public Health and Epidemiology, Vanbandhu
College of Veterinary Science and Animal
Husbandry,
Navsari Agricultural University, Navsari - 396 450, Gujarat,
India; cv_vet@rediffmail.com
I. H.
Kalyani:
Department of Veterinary Microbiology, Vanbandhu College of
Veterinary Science and Animal Husbandry, Navsari Agricultural
University, Navsari - 396 450, Gujarat, India;
ihkvet@gmail.com
Rajeev
Kumar: Department
of Veterinary Public Health and Epidemiology, Vanbandhu College of
Veterinary Science and Animal
Husbandry,
Navsari Agricultural University, Navsari - 396 450, Gujarat,
India; rajeevkumar@nau.in
D. P.
Kshirsagar: Department of Veterinary Public Health and
Epidemiology, Vanbandhu College of Veterinary Science and Animal
Husbandry,
Navsari Agricultural University, Navsari - 396 450, Gujarat,
India;
drdpk04v@gmail.com
Received: 21-11-2014, Revised: 15-04-2015, Accepted:
25-04-2015, Published online: 06-06-2015
Corresponding author:
Deepti N. Nayak,
e-mail: drdeeptin@rediffmail.com
Citation:
Nayak DN, Savalia CV,
Kalyani IH, Kumar R, Kshirsagar DP (2015) Isolation,
identification, and characterization of Listeria spp. from
various animal origin foods, Veterinary World 8(6):
695-701.
Abstract
Aim:
The present study was
undertaken with the prime objective of isolating and identifying
Listeria
spp. from various foods of animal origin
sold at retail market outlets in the city of Navsari, Gujarat.
Materials and Methods: Total
200 samples comprising of milk, milk products, meat, and fish (50
each) collected aseptically from local market which were subjected
first to pre-enrichment in half strength Fraser broth followed by
enrichment in full strength Fraser broth and subsequent plating on
PALCAM agar. The growth with the typical colony characteristics
were further identified up to species level on the basis of their
morphological and biochemical characteristics. Cultures identified
as Listeria monocytogenes
were further subjected to
in vitro
pathogenicity tests and detection of different virulence
associated genes viz. actA,
hlyA,
and iap
using polymerase chain reaction.
Results: Of the total 200 food
samples of animal origin; 18 (9%) were found positive for
Listeria spp.
which were identified as
Listeria seeligeri
(6, 33.3%),
Listeria innocua (5, 27.7%),
Listeria welshimeri (4,
22.2%), and L. monocytogenes
(3, 16.6%). The highest prevalence was
observed in milk samples (8). Species wise, 6 isolates of
L. seeligeri
which included two each from cow milk,
buffalo milk, and meat samples; 5 L.
innocua isolates included four
recovered from fish and one from meat sample; 4 L.
welshimeri comprised of two
isolates from ice cream and one each from buffalo milk and meat
sample; and 3 isolates of L.
monocytogenes recovered from
milk (1 cow and 2 buffalo milk). All 3 L.
monocytogenes isolates
screened for the presence of virulence genes
viz.
actA,
hlyA, and
iap
using the specific primers revealed the presence of all the genes
suggesting the possibility of danger of foodborne listeriosis
among raw milk consumers.
Conclusion: Listeria
spp.
was isolated from 9% (18/200) of the animal origin food samples
viz.;
milk, milk products, meat, and fish with the highest prevalence in
the milk samples. L. monocytogenes
was isolated from 3 milk samples
only. L. seeligeri
was the predominant species isolated
followed by L. innocua, L. welshimeri,
and L. monocytogenes in this
study. L. monocytogenes
were found to carry virulence genes like
actA,
hly A,
and iap
genes suggesting the pathogenic
potential of these isolates.
Keywords:
animal origin foods, Listeria
monocytogenes,
Listeria
spp., polymerase chain reaction, virulence genes.
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