| 
              
              
              Open Access  
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
 
 
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, 
distribution and reproduction in any medium, provided the work is properly 
cited. 
 
              
              
              Research 
              
              
(Published online: 
              21-06-2015) 
              
              
              14.  
              
              Emerging status of anaplasmosis in cattle in Hisar - 
              Tarun Kumar, Neelesh Sindhu, Gaurav Charaya, Ankit Kumar, Parmod 
              Kumar, Gauri Chandratere, Divya Agnihotri and Rajesh Khurana 
              
              Veterinary World, 8(6): 768-771   
              
   
                
                
doi: 
              10.14202/vetworld.2015.768-771   
              Tarun Kumar: 
              Teaching Veterinary Clinical Complex, Lala Lajpat Rai University 
              of Veterinary & Animal Sciences, Hisar - 125 004, 
              Haryana, India, tarunvet@gmail.com 
              Neelesh Sindhu: 
              Teaching Veterinary Clinical Complex, Lala Lajpat Rai University 
              of Veterinary & Animal Sciences, Hisar - 125 004, 
              Haryana, India, drneeleshsindhu@yahoo.com 
              Gaurav Charaya: 
              Department of Veterinary Medicine, Lala Lajpat Rai University of 
              Veterinary & Animal Sciences, Hisar - 
              125 004, Haryana, India, gcharaya9@gmail.com 
              Ankit Kumar: 
              Department of Veterinary Medicine, Lala Lajpat Rai University of 
              Veterinary & Animal Sciences, Hisar - 
              125 004, Haryana, India; ankitk813@gmail.com 
              Parmod Kumar: 
              Department of Veterinary Medicine, Lala Lajpat Rai University of 
              Veterinary & Animal Sciences, Hisar - 
              125 004, Haryana, India; pdhuran09@gmail.com 
              Gauri Chandratere: 
              Department of 
              Veterinary Pathology, Lala Lajpat Rai University of Veterinary & 
              Animal 
              Sciences, Hisar - 125 004, Haryana, India; drgaurichandratre@gmail.com 
              Divya Agnihotri: 
              Teaching Veterinary Clinical Complex, Lala Lajpat Rai University 
              of Veterinary & Animal Sciences, Hisar - 125 004, 
              Haryana, India; dr_divya_agnihotri@yahoo.co.in and 
              Rajesh Khurana; 
              Teaching Veterinary Clinical Complex, Lala Lajpat Rai University 
              of Veterinary & Animal Sciences, Hisar - 125 004, 
              Haryana, India; khurana.rajesh846@gmail.com   
              Received: 04-02-2015, Revised: 14-05-2015, Accepted: 21-05-2015, 
              Published online: 21-06-2015   
              
              
              Corresponding author: 
              Tarun Kumar, e-mail: 
              tarunvet@gmail.com 
 
              Citation:Kumar T, Sindhu N, 
              Charaya G, Kumar A, Kumar P, Chandratere G, Agnihotri D, Khurana R 
              (2015) Emerging status of anaplasmosis in cattle in Hisar, 
              Veterinary World 8(6):768-771. 
 
              Abstract 
 Aim:
              Anaplasma marginale is a rickettsial pathogen 
              responsible for progressive anemia in ruminants leading to huge 
              economic losses. The objectives of this cross-sectional study were 
              to determine the prevalence of anaplasmosis and therapeutic 
              evaluation of traditional line of treatment. 
              Materials and Methods: A total of 168 cattle presented to 
              Teaching Veterinary Clinical Complex, Lala Lajpat Rai University 
              of Veterinary & Animal Sciences, Hisar during the period of 3 
              months (July-September, 2014) with history of fever, anorexia, 
              reduced milk yield and tick infestation were analyzed for 
              prevalence of hemoprotozoan diseases using classical giemsa 
              stained thin blood smear parasitological method. 
              Results: Out of these 168 animals, 7 (4.17%) were found to be 
              suffering from anaplasmosis on the basis of presence of dense, 
              rounded, intra-erythrocytic bodies situated on or near the margin 
              of the erythrocytes. Overall prevalence of theileriosis and 
              babesiosis were found to be 42.9% and 1.8%, respectively. Level of 
              parasitemia was noticed to be 1.2%, 0.8% and 0.9% in babesiosis, 
              theileriosis, and anaplasmosis, respectively. The most marked and 
              common clinical signs reported in all the cases were severe anemia 
              (hemoglobin=3-6 g/dl) and history of fever, followed by normal 
              body temperature. Following treatment with oxytetracycline 
              parenterally along with supportive therapy out of seven cases six 
              got recovered without any side-effects. 
              Conclusion: The current study indicates the emerging status of 
              anaplasmosis in this part of the country as during the past few 
              years there are very few reports showing the prevalence of 
              clinical cases of anaplasmosis. Treatment with oxytetracycline 
              yielded excellent result showing recovery in most of the clinical 
              cases. 
              Keywords: anaplasmosis, giemsa staining, 
              hemoprotozoan, oxytetracycline. 
 
              References 
 
                
                  | 1. Rajput, Z.I., Hu S.H., Arijo, A.G., Habib, M. and Khalid, 
                  M.J. (2005) Comparative study of Anaplasma parasites in tick 
                  carrying buffaloes and cattle. J. Zhejiang. Univ. Sci. 6: 
                  1057-1062. http://dx.doi.org/10.1631/jzus.2005.B1057
 PMid:16252338 PMCid:PMC1390651
 |  
                  |  |  
                  | 2. Marchette, N. and Stiller, D. (1982) The anaplasmataceae, 
                  Bartonellaceae, and Rochalimaea quintana. In: Marchette, N.J., 
                  editor. Ecological Relationships and Evolution in the 
                  Rickettsiae. CRC Press, Boca Raton, Florida. p98-106. |  
                  |  |  
                  | 3. Kocan, K.M., De La Fuente, J., Blouin, E.F. and Garica-Garcia, 
                  J.C. (2004) Anaplasma marginale (Rickettsiales: 
                  Anaplasmataceae): Recent advances in defining host-pathogen 
                  adaptations of a tick-borne rickettsia. Parasitology., 129: 
                  285-300. http://dx.doi.org/10.1017/s0031182003004700
 |  
                  |  |  
                  | 4. Jongejan, F. and Uilenberg, G. (2004) The global importance 
                  of ticks. Parasitology., 129: S3-S14. http://dx.doi.org/10.1017/S0031182004005967
 PMid:15938502
 |  
                  |  |  
                  | 5. Aubry, P. and Geale, D.W. (2011) A review of bovine 
                  anaplasmosis. Transbound. Emerg. Dis., 58(1): 1-30. http://dx.doi.org/10.1111/j.1865-1682.2010.01173.x
 PMid:21040509
 |  
                  |  |  
                  | 6. Richey, E.J. and Palmer, G.H. (1990) Bovine anaplasmosis. 
                  Compend. Contin. Educ. Pract. Vet., 12: 1661-1668. |  
                  |  |  
                  | 7. Radostitis, O.M., Gay, C.C., Blood, D.C. and Hinchliff, 
                  K.W. (2000) In: Veterinary Medicine. A Text Book of the 
                  Diseases of Cattle, Sheep, Goats and Horses. 9th ed. WB 
                  Saunders, London, New York, Philadelephia. p1261-1265. |  
                  |  |  
                  | 8. OIE. (2008) Terrestrial Manual, Bovine Anaplasmosis. OIE, 
                  Paris, France. |  
                  |  |  
                  | 9. Rodríguez, S.D., Ortiz, M.A., Ocampo, R.J. and Murguía, 
                  C.A. (2009) Molecular epi-demiology of bovine anaplasmosis 
                  with a particular focus in Mexico. Infect. Genet. Evol., 9: 
                  1092-1101. http://dx.doi.org/10.1016/j.meegid.2009.09.007
 PMid:19786123
 |  
                  |  |  
                  | 10. Futse, J.E., Ueti, M.W., Knowles, D.P. and Palmer, G.H. 
                  (2003) Transmission of Anaplasma marginale by Boophilus 
                  microplus: Retention of vector competence Boophilus microplus: 
                  Retention of vector competence. J. Clin. Mic., 41: 3829-3834. http://dx.doi.org/10.1128/JCM.41.8.3829-3834.2003
 PMCid:PMC179812
 |  
                  |  |  
                  | 11. Khan, M.Q., Zahoor, A., Jahangir, M. and Mirza, M.A. 
                  (2004) Prevalence of blood parasites in cattle and buffaloes. 
                  Pak. Vet. J., 24: 193-195. |  
                  |  |  
                  | 12. Atif, F.A., Khan, S., Iqbal, H.J. and Roheen, T. (2012) 
                  Prevalence of tick-borne diseases in Punjab, Pakistan and 
                  hematological profile of Anaplasma marginale infection in 
                  indigenous and crossbred cattle. Pak. J. Sci., 64: 11-15. |  
                  |  |  
                  | 13. Sajid, M.S., Siddique, R.M., Khan, S.A., Iqbal Z. and 
                  Khan, M.N. (2014) Prevalence and risk factors of anaplasmosis 
                  in cattle and buffalo populations of district Khanewal, 
                  Punjab, Pakistan. Glob. Vet., 12(1): 146-153. |  
                  |  |  
                  | 14. Ashuma ., Sharma, A., Singla, L.D., Kaur, P., Bal, M.S., 
                  Batth, B.K. and Juyal, P.D. (2013) Prevalence and 
                  haemato-biochemical profile of anaplasma marginale infection 
                  in dairy animals of Punjab (India). Asian Pac. J. Trop. Med., 
                  6: 139-144. |  
                  |  |  
                  | 15. Prasanna, P.K. and Sangwan, A.K. (2010) Comparative 
                  prevalence of subclinical bovine anaplasmosis under different 
                  cattle management systems in Haryana. Haryana Vet., 49: 1-5. |  |