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R esearch
(Published online:
28-06-2015)
19.
Effect of preputial washing on bacterial
load and preservability of semen in Murrah buffalo bulls -
G. S. Meena, V. S. Raina, A. K. Gupta, T. K. Mohanty, M.
Bhakat, M. Abdullah and R. Bishist
Veterinary World, 8(6): 798-803
doi:
10.14202/vetworld.2015.798-803
G. S.
Meena:
Krishi Vigyan Kendra, Bundi-323001, Rajasthan, India;
ghanshyam774@gmail.com
V. S.
Raina:
Artificial Breeding Research Centre, ICAR-National Dairy Research
Institute, Karnal - 132 001, Haryana, India; vsrainandri@gmail.com
A. K.
Gupta:
Artificial Breeding Research Centre, ICAR-National Dairy Research
Institute, Karnal - 132 001, Haryana, India; guptaak2009@gmail.com
T. K.
Mohanty:
Artificial Breeding Research Centre, ICAR-National Dairy Research
Institute, Karnal - 132 001, Haryana, India; mohanty.tushar@gmail.com
M.
Bhakat:
Artificial Breeding Research Centre, ICAR-National Dairy Research
Institute, Karnal - 132 001, Haryana, India;
bhakat.mukesh@gmail.com
M.
Abdullah:
Artificial Breeding Research Centre, ICAR-National Dairy Research
Institute, Karnal - 132 001, Haryana, India; mabhatndri@gmail.com
R.
Bishist: Dr. Yashwant Singh Parmar University of Horticulture
& Forestry, Nauni, Solan-173230, Himachal Pradesh, India;
rohit.ndri@gmail.com
Received: 26-12-2015, Revised: 21-05-2015, Accepted: 29-05-2015,
Published online: 28-06-2015
Corresponding author:
M. Bhakat, e-mail: bhakat.mukesh@gmail.com
Citation:
Meena GS, Raina VS,
Gupta AK, Mohanty TK, Bhakat M, Abdullah M, Bishist R (2015)
Effect of preputial washing on bacterial load and preservability
of semen in Murrah buffalo bulls, Veterinary World 8(6):
798-803.
Abstract
Aim:
To study the effect of preputial washing on bacterial load,
preservability and semen quality in Murrah buffalo bulls
Materials and Methods: A total of 36 collections of three
Murrah buffalo bulls maintained at Artificial Breeding Research
Centre, ICAR-National Dairy Research Institute, Karnal, were
collected at weekly intervals from each bull without preputial
washing and latter ejaculates from same bull with preputial
washing by infusing normal saline (0.85%), KMnO 4
(0.02%) and savlon (2.0%) to first, second and
third bull, respectively. The microbial load and semen quality
were evaluated during different hours of storage at refrigerated
temperature (0, 24 and 48 h) and after thrawing of cryopreserved
(at −196°C)
semen.
Results: The results of preservation of semen at refrigerated
temperature showed that bacterial load was markedly lower in
ejaculates of bulls subjected to preputial washing. Semen
preserved at refrigerator temperature and cryopreserved, the
effect of washing solution was significant for individual motility
(IM), non-eosiniphilic count, hypo-osmotic swelling reactivity
(HOST), total plate count (TPC) and acrosome integrity. KMnO 4
was found to be the best in lowering bacterial
load, sperm abnormalities and in improving semen quality such as
motility, non-eosinophilic count, HOST and acrosome integrity even
up to 48 h of preservation and cryopreserved semen. Effect of
duration of preservation and stage of cryopreservation was also
significant for IM, non-eosiniphilic count, HOST, sperm
abnormalities and acrosome integrity.
Conclusion: Overall the results suggested
that preputial washing with KMnO4 solution
improved the semen quality and reduced microbial load of Murrah
buffalo bull’s semen preserved at refrigerated temperature and
cryopreservation.
Keywords: preputial washing, bacterial load,
semen quality, preservability, Murrah buffalo bull.
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