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R esearch
(Published online: 04-03-2015)
1.
Pock forming ability of fowl pox virus isolated from layer chicken
and its adaptation in chicken embryo fibroblast cell culture
-
Varsha Rani Gilhare, S. D. Hirpurkar, Ashish Kumar, Surendra Kumar
Naik and Tarini Sahu
Veterinary World, 8(3): 245-250
doi:
10.14202/vetworld.2015.245-250
Varsha
Rani Gilhare:
Department of Veterinary Microbiology, College of Veterinary
Science and Animal Husbandry, Anjora, Durg, Chhattisgarh, India;
docvarsha20@gmail.com
S. D.
Hirpurkar:
Department of Veterinary Microbiology, College of Veterinary
Science and Animal Husbandry, Anjora, Durg, Chhattisgarh, India;
smpuhir@yahoo.com
Ashish
Kumar:
Department of Veterinary Microbiology, College of Veterinary
Science and Animal Husbandry, Anjora, Durg, Chhattisgarh, India;
ashishvetdoc@gmail.com
Surendra Kumar Naik:
Department of Animal Nutrition, College of Veterinary Science and
Animal Husbandry, Anjora, Durg, Chhattisgarh, India;
drsurendranaikdurg@gmail.com
Tarini
Sahu: Department of Animal Nutrition, College of Veterinary
Science and Animal Husbandry, Anjora, Durg, Chhattisgarh, India;
drtarinisahu@gmail.com
Received: 09-10-2014, Revised: 13-01-2015, Accepted: 20-01-2015,
Published online: 04-03-2015
Corresponding author:
Varsha Rani Gilhare, e-mail: docvarsha20@gmail.com
Citation:
Gilhare VR,
Hirpurkar SD, Kumar A, Naik SK, Sahu T (2015) Pock forming ability
of fowl pox virus isolated from layer chicken and its adaptation
in chicken embryo fibroblast cell culture, Veterinary World 8(3):245-250.
Abstract
Aim:
The objective of the present
study was to examine pock forming ability of field strain and
vaccine strain of fowl pox virus (FPV) in chorioallantoic membrane
(CAM) of embryonated chicken eggs and its adaptation in chicken
embryo fibroblast (CEF) cell culture.
Materials and Methods: Dry
scabs were collected from 25 affected birds in glycerin-saline and
preserved at 4°C until processed. Virus was isolated in 10-day-old
embryonated chicken eggs by dropped CAM method. The identity of
the virus is confirmed by clinical findings of affected birds,
pock morphology and histopathology of infected CAM. In addition
one field isolate and vaccine strain of FPV was adapted to CEF
cell culture. CEF cell culture was prepared from 9-day-old
embryonated chicken eggs.
Result:
Clinical symptoms observed in affected birds include pox lesion on
comb, wattle, eyelids and legs, no internal lesions were observed.
All field isolates produced similar findings in CAM. Pocks
produced by field isolates ranged from 3 mm to 5 mm at the third
passage while initial passages edematous thickening and necrosis
of CAM was observed. Pocks formed by lyophilized strain were
ranges from 0.5 mm to 2.5 mm in diameter scattered all over the
membrane at the first passage. Intra-cytoplasmic inclusion bodies
are found on histopathology of CAM. At third passage level, the
CEF inoculated with FPV showed characteristic cytopathic effect (CPE)
included aggregation of cells, syncytia and plaque formation.
Conclusion:
FPV field isolates and vaccine strain produced distinct pock
lesions on CAMs. Infected CAM showed intracytoplasmic inclusion
bodies. The CEF inoculated with FPV field isolate as well as a
vaccine strain showed characteristic CPE at third passage level.
Keywords:
chicken embryo fibroblast cell culture, chorioallantoic membrane,
embryonated egg, fowl pox virus.
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