| 
              
              
              Open Access  
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
 
 
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, 
distribution and reproduction in any medium, provided the work is properly 
cited. 
 
              
              
              Research 
              
(Published online: 
              16-03-2015) 
              
              12.
              
              Variations of motility and survival with 
              storage time at 4°C of epididymal spermatozoa Ouled-Djellal breed 
              rams in Eastern Algeria - B. Safsaf, S. Belkadi, L. 
              Belkacem, B. Mamache and M. Tlidjane 
              
              Veterinary World, 8(3): 326-329   
              
   
                
                
doi: 
              10.14202/vetworld.2015.326-329   B. 
              Safsaf: 
              Department of Veterinary Science, Laboratory ESPA, Veterinary and 
              Agricultural Sciences Institute, Hadj Lakhdar University, Batna - 
              05000, Algeria;
              
              
              saf2_b@yahoo.fr S. 
              Belkadi: 
              Department of Veterinary Science, Laboratory ESPA, Veterinary and 
              Agricultural Sciences Institute, Hadj Lakhdar University, Batna - 
              05000, Algeria;
              
              
              souhilabelkadi@gmail.com L. 
              Belkacem: 
              Department of Veterinary Science, Laboratory ESPA, Veterinary and 
              Agricultural Sciences Institute, Hadj Lakhdar University, Batna - 
              05000, Algeria;
              
              
              lbelkacem@yahoo.fr B. 
              Mamache: 
              Department of Veterinary Science, Laboratory ESPA, Veterinary and 
              Agricultural Sciences Institute, Hadj Lakhdar University, Batna - 
              05000, Algeria;
              
              
              mamachebakir@yahoo.fr M. 
              Tlidjane: Department of Veterinary Science, Laboratory ESPA, 
              Veterinary and Agricultural Sciences Institute, Hadj Lakhdar 
              University, Batna - 05000, Algeria;
              
              ayathir@yahoo.fr   
              Received: 14-11-2014, Revised: 28-01-2015, Accepted: 05-02-2015, 
              Published online: 16-03-2015   
              
              
              Corresponding author: 
              
              B. Safsaf, e-mail: saf2_b@yahoo.fr 
 
              Citation:Safsaf B, Belkadi S, 
              Belkacem L, Mamache B, Tlidjane M (2015) Variations of motility 
              and survival with storage time at 4°C of epididymal spermatozoa 
              Ouled-Djellal breed rams in Eastern Algeria, Veterinary World
              8(3):326-329. 
 
              Abstract 
 Aim:
              The aim of this study was to evaluate some reproduction 
              performances in Ouled-Djellal rams. 
              Materials and Methods: This study involved genital organs 
              removed after slaughter from 54 rams at the municipal 
              slaughterhouse of Batna (East Algeria). 
              Results: The measurements of survival and mobility of 
              epididymal sperm followed at 0, 24, 48 and 72 h after collection, 
              revealed significant (p<0.05) to highly significant differences 
              (p>0.001) according to time. Thus, concerning the sperm motility 
              the values were 91.00±2.40%, 89.20±2.40%, 77.00±6.20% and 
              62.60±1.20% at 0, 24, 48 and 72 h, respectively. Indeed, in live 
              sperm, the viability rates were 82.15±1.48%, 77.67±1.74%, 
              66.56±1.95% and 52.30±1.46% at 0, 24, 48 and 72 h, respectively. 
              Conclusion: This study revealed that epididymal spermatozoa 
              stored at 04°C for 72 h kept their mobility and vitality at nearly 
              a half of their the original parameters. 
              Keywords: breed ouled-djellal, epididymal 
              sperm, rams, storage at 4°C 
 
              References 
 
                
                  | 1. MADR (Ministry of Agriculture and Rural Development- 
                  Algeria). Algeria Report (1). Current situation of breeding 
                  camels in arid and semi-arid areas. In: International Workshop 
                  on: The impact of climate change on livestock and sustainable 
                  rangeland management in arid and semi-arid areas of North 
                  Africa. Ouargla 21-24 November 2011. |  
                  |  |  
                  | 2. Mukasa-Mugerwa, E. and Ezaz, Z. (1992) Relationship of 
                  testicular growth and size to age, body weight and onset of 
                  puberty in Menzram lambs. Theriogenology, 38: 979-988. http://dx.doi.org/10.1016/0093-691X(92)90172-N
 |  
                  |  |  
                  | 3. Ehling, C., Rath, D., Struckmann, C., Frenzel, A., 
                  Schindler, L. and Niemann, H. (2006) Utilization of 
                  frozen-thawed epididymal ram semen to preserve genetic 
                  diversity in Scrapie susceptible sheep breeds. Theriogenology, 
                  66, 2160-2164. http://dx.doi.org/10.1016/j.theriogenology.2006.07.003
 PMid:16905182
 |  
                  |  |  
                  | 4. Kaabi, M., Paz, P., Alvarez, M., Anel, E., Boixo, JC., 
                  Rouissi, H., Herraez, P. and Anel, L. (2003) Effect of 
                  epididymis handling conditions on the quality of ram 
                  spermatozoa recovered post-mortem. Theriogenology, 60(7): 
                  1249-1259. http://dx.doi.org/10.1016/S0093-691X(03)00139-0
 |  
                  |  |  
                  | 5. Martinez-Pastor, F., Diaz-Corujo, AR., Anel, E., Herraez, 
                  P., Anel, L. and de Paz, P. (2005) Post mortem time and season 
                  alter subpopulation characteristics of Iberian red deer 
                  epididymal sperm. Theriogenology, 64: 958-974. http://dx.doi.org/10.1016/j.theriogenology.2005.01.003
 PMid:16054499
 |  
                  |  |  
                  | 6. Álvarez, M., Tamayo-Canul, J., Martínez-Rodrígueza, C., 
                  López-Uruena, E., Gomes-Alves, S., Anel, L., Martínez-Pastor, 
                  F. and de Paz, P. (2012) Specificity of the extender used for 
                  freezing ram sperm depends of the spermatozoa source 
                  (ejaculate, electroejaculate or epididymis). Anim. Reprod. 
                  Sci., 132(3-4): 145-154. http://dx.doi.org/10.1016/j.anireprosci.2012.05.006
 PMid:22673394
 |  
                  |  |  
                  | 7. Braun, J., Sakai, M., Hochi, S. and Oguri, N. (1994) 
                  Preservation of ejaculated and epididymal stallion spermatozoa 
                  by cooling and freezing. Theriogenology, 41(4): 809-818. http://dx.doi.org/10.1016/0093-691X(94)90497-7
 |  
                  |  |  
                  | 8. Songsasen, N., Tong, J. and Leibo, S. (1998) Birth of live 
                  mice derived by in vitro fertilization with spermatozoa 
                  retrieved up to twenty-four hours after death. J. Exp. Zool., 
                  280(2): 189-196. http://dx.doi.org/10.1002/(SICI)1097-010X(19980201)280:2<189::AID-JEZ10>3.0.CO;2-H
 |  
                  |  |  
                  | 9. Lambrechts, H., van Niekerk, F.E., Coetzer, W.A., Cloete, 
                  S.W.P. and van der Horst, G. (1999) The effect of 
                  cryopreservation on the survivability, viability and motility 
                  of epididymal African buffalo (Svnceruscaffer) spermatozoa. 
                  Theriogenology, 52: 1241-1249. http://dx.doi.org/10.1016/S0093-691X(99)00201-0
 |  
                  |  |  
                  | 10. Nichi, M., Rijsselare, T., Van Soom, A., De Clercq, 
                  J.B.P., Goovaerts, I.G.F., Barnabe, V.H. and Bols, P.E.J. 
                  (2007) Effect of bull epididymis storage conditions on 
                  cryopreserved epididymal sperm in vitro fertility and lipid 
                  peroxidation status. 33rdAnnual International Embryo Transfer 
                  Society. 7-9 Jan., Kyoto, Japan. Reprod. Fertil. Dev., 19: 
                  126. http://dx.doi.org/10.1071/RDv19n1Ab15
 |  
                  |  |  
                  | 11. Anel, L., Alvarez, M., Anel, E., Martinez-Pastor, F., 
                  Martinez, F., Chamorro, C. and de Paz, P. (2011) Evaluation of 
                  three different extenders for use in emergency salvaging of 
                  epididymal spermatozoa from a Cantabric brown bear. Reprod. 
                  Domest. Anim., 46: 85-90. http://dx.doi.org/10.1111/j.1439-0531.2010.01646.x
 PMid:20546173
 |  
                  |  |  
                  | 12. Blash, S., Melican, D. and Gavin, W. (2000) 
                  Cryopreservation of epididymal sperm obtainedat necropsy from 
                  goats. Theriogenology, 54: 899-905. http://dx.doi.org/10.1016/S0093-691X(00)00400-3
 |  
                  |  |  
                  | 13. Kundu, C.N., Chakraborty, J., Dutta, P., Bhattacharyya, 
                  D., Ghosh, A. and Majumder, G.C. (2000) Development of a 
                  simple sperm cryopreservation model using a chemically defined 
                  medium and goat cauda epididymal spermatozoa. Cryobiology, 40: 
                  117-125. http://dx.doi.org/10.1006/cryo.2000.2230
 PMid:10788311
 |  
                  |  |  
                  | 14. Tamayo-Canul, J., Alvareza, M., López-Uruena, E., Nicolas, 
                  M., Martinez-Pastora, F., Anel, E., Anel, L. and de Paz, P. 
                  (2011) Undiluted or extended storage of ram epididymal 
                  spermatozoa as alternatives to refrigerating the whole 
                  epididymes. Anim. Reprod. Sci., 126: 76-82. http://dx.doi.org/10.1016/j.anireprosci.2011.04.011
 PMid:21601384
 |  
                  |  |  
                  | 15. Fernández-Santos, M.R., Soler, A.J., Ramón, M., 
                  Ros-Santaella, J.L., Maroto-Morales, A., García-Álvarez, O., 
                  Bisbal, A., Garde, J.J., Coloma, M.A. and Santiago-Moreno J. 
                  (2011) Effect of post-mortem time on post-thaw characteristics 
                  of Spanish ibex (Capra pyrenaica) spermatozoa. Anim. Reprod. 
                  Sci., 129(1-2): 56-66. http://dx.doi.org/10.1016/j.anireprosci.2011.09.011
 PMid:22014412
 |  
                  |  |  
                  | 16. Guérin, Y., Locatelli, Y., Comizolli, P., Mauget, R., 
                  Mermillod, P., Legendre, X., Gatti, J.L. and Dacheux, J.L. 
                  (2003) Conservation et utilisation du sperme épididymaire 
                  d'ovins et de cervidés en insémination artificielle et 
                  fécondation in vitro. Les Actes du BRG, 4: 173-183. |  
                  |  |  
                  | 17. Hishinuma, M. and Sekine, J. (2003) Evaluation of membrane 
                  integrity of canine epididymal spermatozoa by short 
                  hypoosmotic swelling test with ultrapure water. J. Vet. Med. 
                  Sci., 65: 817-820. http://dx.doi.org/10.1292/jvms.65.817
 PMid:12939512
 |  
                  |  |  
                  | 18. Martinez-Pastor, F., Guerra, C., Kaabi, M., Diaz, A.R., 
                  Anel, E., Herraez, P., de Paz, P. and Anel, L. (2005) Decay of 
                  sperm obtained from epididymes of wild ruminants depending on 
                  postmortem time. Theriogenology, 63(1): 24-40. http://dx.doi.org/10.1016/j.theriogenology.2004.03.003
 PMid:15589271
 |  
                  |  |  
                  | 19. Varisli, O., Uguz, C., Agca, C. and Agca, Y. (2009) 
                  Motility and acrosomal integrity comparisons between 
                  electro-ejaculated and epididymal ram sperm after exposure to 
                  a range of anisosmotic solutions, cryoprotective agents and 
                  low temperatures. Anim. Reprod. Sci., 110: 256-268. http://dx.doi.org/10.1016/j.anireprosci.2008.01.012
 PMid:18294786
 |  
                  |  |  
                  | 20. Barth, A. and Oko, R. (1989) Abnormal Morphology of Bovine 
                  Spermatozoa. 1st ed. Iowa State University Press, Ames, Iowa. |  
                  |  |  
                  | 21. Eddy, E.M. (2006) Spermatozoon. In: Neill, J.D. and 
                  editor. Knobil and Neill's Physiology of Reproduction. 3rd ed. 
                  Elsevier, St Louis, Missouri. p3-55. |  
                  |  |  
                  | 22. Robaire, B., Hinton, B.T. and Orgebin-Crist, MC. (2006) 
                  The epididymis. In: Neill, J.D. and editor. Knobil and Neill's 
                  Physiology of Reproduction. 3rd ed. Elsevier, St Louis, 
                  Missouri. p1071-1148. |  
                  |  |  
                  | 23. Martins, C.F., Driessen, K., Melo Costa, P., 
                  Carvalho-Neto, J.O., de Sousa, R.V., Rumpf, R. and Dode, M.N. 
                  (2009) Recovery, cryopreservation and fertilization potential 
                  of bovine spermatozoa obtained from epididymides stored at 5 C 
                  by different periods of time. Anim. Reprod. Sci., 116(1-2): 
                  50-57. http://dx.doi.org/10.1016/j.anireprosci.2008.12.018
 PMid:19181463
 |  
                  |  |  
                  | 24. Lone, F.A., Islam, R., Khan, M.Z. and SofiK.A. (2011) 
                  Effect of transportation temperature on the quality of cauda 
                  epididymal spermatozoa of ram. Anim. Reprod. Sci., 123(1-2): 
                  54-59. http://dx.doi.org/10.1016/j.anireprosci.2010.10.012
 PMid:21131148
 |  
                  |  |  
                  | 25. Mir, S.S., Lone, F.A., Khan, M.Z., Malik, A.A., Islam R. 
                  and Sofi K.A. (2012) Effect of cold storage period on the 
                  quality of ram cauda epididymal spermatozoa recovered 
                  postmortem. Turk. J. Vet. Anim. Sci., 36(6): 683-687. |  
                  |  |  
                  | 26. Rath, D. and Niemann, H. (1997) In vitro fertilization of 
                  porcine oocytes with fresh and frozen-thawed ejaculated or 
                  frozenthawed epididymal semen obtained from identical boars. 
                  Theriogenology, 47(4): 785-793. http://dx.doi.org/10.1016/S0093-691X(97)00034-4
 |  
                  |  |  
                  | 27. Rickard, J.P., Pini, T., Soleilhavoup, C., Cognié, J., 
                  Bathgate, R., Lynch, GW., Evans, G., Maxwell, W.M., Druart, X. 
                  and de Graaf S. (2014) Seminal plasma aids the survival and 
                  cervical transit of epididymal ram spermatozoa. Reproduction, 
                  148(5): 469-478. http://dx.doi.org/10.1530/REP-14-0285
 PMid:25118301
 |  
                  |  |  
                  | 28. Hishinuma, M., Suzuki, K. and Sekine, J. (2003) Recovery 
                  and cryopreservation of sika deer (Cervusnippon) spermatozoa 
                  from epididymides stored at 48C. Theriogenology, 59(3-4): 
                  813-820. http://dx.doi.org/10.1016/S0093-691X(02)01154-8
 |  |