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R esearch
(Published online:
18-03-2015)
17.
Antibacterial efficacy of ethyl
acetate fraction of Psidium guajava leaf aqueous extract on
experimental Escherichia coli (O78)
infection in
chickens
- Y. A. Geidam, A. G.
Ambali, P. A. Onyeyili, M. B. Tijjani, H. I. Gambo and I. A.
Gulani
Veterinary World, 8(3): 358-362
doi:
10.14202/vetworld.2015.358-362
Y. A.
Geidam:
Department of Veterinary Medicine, Faculty of Veterinary Medicine,
University of Maiduguri, P.M.B. 1069, Maiduguri, Borno State,
Nigeria;
A. G.
Ambali:
Department of Veterinary Medicine, Faculty of Veterinary Medicine,
University of Ilorin, Kwara State, Nigeria; aambali076@yahoo.com
P. A.
Onyeyili:
Department of Veterinary Physiology, Pharmacology and
Biochemistry, College of Veterinary Medicine, Federal University
of Agriculture, Makurdi, Benue State, Nigeria;
onyeyili@gmail.com
M. B.
Tijjani:
Department of Veterinary Physiology, Pharmacology and
Biochemistry, Faculty of Veterinary Medicine, University of
Maiduguri, P.M.B. 1069, Maiduguri, Borno State, Nigeria;
bashvet@yahoo.com
H. I.
Gambo:
Department of Veterinary Pathology, Faculty of Veterinary
Medicine, University of Maiduguri, P.M.B. 1069, Maiduguri, Borno
State, Nigeria;
hgambo24@yahoo.com
I. A.
Gulani: Department of Veterinary Medicine, Faculty of
Veterinary Medicine, University of Maiduguri, P.M.B. 1069,
Maiduguri, Borno State, Nigeria;
gulaniisa@yahoo.com
Received:
28-11-2014, Revised: 10-02-2015, Accepted: 16-02-2015, Published
online: 18-03-2015
Corresponding author:
Y.A. Geidam, email: ygeidam@yahoo.com
Citation:
Geidam YA, Ambali AG,
Onyeyili PA, Tijjani MB, Gambo HI, Gulani IA (2015) Antibacterial
efficacy of ethyl acetate fraction of Psidium guajava leaf aqueous
extract on experimental Escherichia coli (O78) infection in
chickens, Veterinary World, 8(3): 358-362.
Abstract
Aim:
This study was desingned to examine the efficacy of ethyl
acetate fraction of aqueous extracted Psidium guajava
leaves on chicks experimentally-infected with diarrheagenic strain
of Escherichia coli O78.
Methods: A total of 60 ISA brown male chicks were randomly
divided into 6 Groups of ten chicks each in separate cages. Group
A was not infected and not treated. Groups B, C and D were
infected and treated with extracts at a dose of 25, 50 and 100
mg/kg respectively for 10 days. Group E was infected and treated
with oxytetracycline while Group F was infected, but left
untreated. Chicks from all groups were closely monitored for
clinical signs, body weight change and fecal bacterial shedding
load during the course of the experiment.
Results: Diarrhea, vents pasted with feces, drop in feed
intake accompanied by slow weight gain and decreased activity was
observed in infected untreated groups. Groups treated with graded
doses of the extract experienced a dose-dependent decreased in
severity of the clinical signs shown compared to the infected
untreated group. Bacterial shedding load was found to be lower in
groups treated with the extract and oxytetracycline than those
without intervention.
Conclusion: Ethyl acetate soluble fraction of leaf extract of
Psidium guajava effectively controlled diarrhea and
decreased the severity of other clinical signs caused by
experimental E. coli infections in chicks.
Keywords: antibacteria, chickens
Escherichia coli, efficacy, Psidium guajava
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