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(Published online: 21-03-2015)
18.
Somatic cell count and alkaline phosphatase
activity in milk for evaluation of mastitis in buffalo -
M. P. Patil, A. S. Nagvekar, S. D. Ingole, S. V. Bharucha and
V. T. Palve
Veterinary World, 8(3): 363-366
doi:
10.14202/vetworld.2015.363-366
M. P.
Patil:
Department of Veterinary Physiology, College of Animal Husbandry,
Sharadanagar, Baramati, Pune, Maharashtra, India;
patilmegha21@gmail.com
A. S.
Nagvekar:
Department of Veterinary Physiology, Bombay Veterinary College,
Maharashtra Animal and Fishery Sciences University, Parel, Mumbai,
Maharashtra, India;
Anagha.nagvekar@gmail.com
S. D.
Ingole:
Department of Veterinary Physiology, Bombay Veterinary College,
Maharashtra Animal and Fishery Sciences University, Parel, Mumbai,
Maharashtra, India;
ingoleshailesh@gmail.com
S. V.
Bharucha:
Department of Veterinary Physiology, Bombay Veterinary College,
Maharashtra Animal and Fishery Sciences University, Parel, Mumbai,
Maharashtra, India;
Simin1972@gmail.com
V. T.
Palve:
Department of Veterinary Physiology, Bombay Veterinary College,
Maharashtra Animal and Fishery Sciences University, Parel, Mumbai,
Maharashtra, India;
drvijaypalve88@gmail.com
Received:
04-10-2014, Revised: 24-01-2014, Accepted: 02-02-2015, Published
online: 21-03-2015
Corresponding author:
M. P. Patil, e-mail: patilmegha21@gmail.com
Citation:
Patil MP, Nagvekar AS,
Ingole SD, Bharucha SV, Palve VT (2015) Somatic cell count and
alkaline phosphatase activity in milk for evaluation of mastitis
in buffalo, Veterinary World 8(3);363-366.
Abstract
Background and Aim: Mastitis is a serious disease of dairy
animals causing great economic losses due to a reduction in milk
yield as well as lowering its nutritive value. The application of
somatic cell count (SCC) and alkaline phosphatase activity in the
milk for diagnosis of mastitis in buffalo is not well documented.
Therefore, the present study was conducted to observe the SCC and
alkaline phosphatase activity for evaluation of mastitis in
buffalo.
Materials and Methods: Milk samples of forty apparently
healthy lactating buffaloes were selected and categorized into
five different groups viz. normal buffaloes, buffaloes with
subclinical mastitis with CMT positive milk samples (+1 Grade),
(+2 Grade), (+3 Grade), and buffaloes with clinical mastitis with
8 animals in each group. The milk samples were analyzed for SCC
and alkaline phosphatase activity.
Results: The levels of SCC (×10 5
cells/ml) and alkaline phosphatase (U/L) in different groups were
viz. normal (3.21±0.179, 16.48±1.432), subclinical mastitis
with CMT positive milk samples with +1 Grade (4.21±0.138,
28.11±1.013), with +2 Grade (6.34±0.183, 34.50±1.034), with +3
Grade (7.96±0.213, 37.73±0.737) and buffaloes with clinical
mastitis (10.21±0.220, 42.37±0.907) respectively, indicating an
increasing trend in the values and the difference observed among
various group was statistically significant.
Conclusion: In conclusion, the results of the present study
indicate that the concentration of milk SCC and alkaline
phosphatase activity was higher in the milk of buffaloes with
mastitis than in the milk of normal buffaloes.
Keywords: alkaline phosphatase, buffalo
milk, mastitis, somatic cell count.
References
1. Sharma, N. (2007) Alternative approach to control
intramammary infection in dairy cows- Review. Asian J. Anim.
Vet. Adv., 2(2): 50-62.
http://dx.doi.org/10.3923/ajava.2007.50.62 |
|
|
|
2. Kitchen, B.J. (1981) Review of the progress of dairy
science: Bovine mastitis: Milk compositional changes and
related diagnostic tests. J Dairy Sci., 64: 167-188. |
|
|
3. Sharma, N., Singh, N.K. and Bhadwal. M.S. (2011)
Relationship of somatic cell count and mastitis: An overview.
Asian Aust. J. Anim. Sci., 24(3): 429-438.
http://dx.doi.org/10.5713/ajas.2011.10233 |
|
|
|
4. Miller, R.H. and Paape, M.J. (1985) Relationship between
milk somatic cell count and milk yield. In: Proceedings Annual
Meeting National Mastitis Council. p60 cited in Sharma et al.,
(2011). |
|
|
5. Harmon, R.J (1994) Physiology of mastitis and factors
affecting somatic cell counts. J. Dairy Sci., 77: 2103-2112.
http://dx.doi.org/10.3168/jds.S0022-0302(94)77153-8 |
|
|
6. Batavani, R.A., Mortaz, E., Falahian, K. and Dawoodi, M.A.
(2003) Study on frequency, etiology and some enzymatic
activities of subclinical ovine mastitis in Uremia. Iran.
Small Rumin. Res., 50: 45-50.
http://dx.doi.org/10.1016/S0921-4488(03)00122-6 |
|
|
7. Babaei, H.I., Sharifan, M., Mansouri-Najand, L., Molaei, M.
and Kheradmand, M. (2007) Assessment of lactate dehydrogenase,
alkaline phosphatase and aspartate aminotransferase activities
in cow's milk as an indicator of subclinical mastitis. Vet.
Res. Commun., 31(4): 419-425.
http://dx.doi.org/10.1007/s11259-007-3539-x
PMid:17268916 |
|
|
|
8. Ibrahim A.M., AL-Humiany, A.A., Mansoor, A.M.A. and Zaki,
H.M. (2011) Epidemiology and microbiological studies on
mastitis in she-camels. Int. J. Microbiol. Res., 2: 18-27. |
|
|
|
9. Schalm, O.W., Carroll, E.J. and Jain, N.C. editors. (1971)
Bovine Mastatis. Lea and Febiger, Philladelphia, PA. |
|
|
|
10. Snedecor, W. and Cochran, W.G. (1998) Statistical Methods.
8th ed. Oxford and IBH Publishing Company, New Delhi. |
|
|
|
11. Silva, I.D. and Silva, K.F.S.T. (1994) Total and
differential cell counts in buffalo milk. Buffalo J. 2:
133-137. |
|
|
12. Dhakal, I.P. (2006) Normal somatic cell count and
subclinical mastitis in Murrah buffaloes. J. Vet. Med. Sci.,
53: 81-6.
http://dx.doi.org/10.1111/j.1439-0450.2006.00918.x |
|
|
13. Moroni, P., Sqoifo, R., Pisoni, G., Bronzo, V.,
Castiqlioni, B. and Boettcher, P.J. (2006) Relationship
between somatic cell count and intramammary infection in
buffaloes. J Dairy Sci., 89(3): 998-1003.
http://dx.doi.org/10.3168/jds.S0022-0302(06)72165-8 |
|
|
|
14. Sharif, A., Ahmed, T., Bilali, M.Q., Yousuf, A. and
Muhammad, G. (2007) Effect of Severity of sub-clinical
mastitis on somatic cell count and lactose contents of buffalo
milk. Pak. Vet. J., 27(3): 142-144. |
|
|
|
15. Kavitha, K.L., Rajesh, K., Suresh, K., Satheesh, K. and
Syama Sunder, N. (2009) Buffalo mastitis- Risk factors.
Buffalo Bull., 28: 3. |
|
|
|
16. Nandi, S. (2010) Enzymatic alteration in buffalo milk
related to udder health status. M. V. Sc Thesis Submitted to
Maharashtra Animal and Fishery Sciences, Nagpur (M.S). |
|
|
|
17. Hussain R, Javed, M.T. and Khan, A. (2012) Changes in some
biochemical parameters and somatic cell counts in the milk of
buffalo and cattle suffering from mastitis. Pak. Vet. J.,
32(3): 418-421. |
|
|
|
18. Cullen, G.A. (1968) Cell counts throughout lactation. Vet.
Rec., 83: 125-128. |
|
|
19. Dohoo, I.R. and Meek, A.H. (1982) Somatic cell counts in
bovine milk. Can. Vet. J., 23: 119-125.
PMid:17422127 PMCid:PMC1790153 |
|
|
|
20. Poutrel, B. and Rainard, P. (1982) Predicting the
probability of quarter infection (by major pathogen) from
somatic cell concentration. Am. J. Vet. Res., 43(7): 1296-1299
cited in Ghodake (2006) M.V.Sc Thesis Submitted to MAFSU,
Nagpur. |
|
|
|
21. Emanuelson, U., Olsson, T., Mattila, T., Astrom, O. and
Holmberg, O. (1988) Effect of parity and stage of lactation on
adenosine triphosphate, somatic cell count and antitrypsin
content in cow's milk. Dairy. Sci., Abstr. 50: 2618 cited in
Managuli, (2011) M. V. Sc thesis submitted to MAFSU, Nagpur. |
|
|
|
22. Nguyen, T.T. (2007) Dairy production, milk quality and
milk somatic cell count on smallholder farms in Hochiminh
city, Vietnam. M.Sc Thesis, Swedish University of Agricultural
Sciences Cited in Managuli (2011) M. V. Sc Thesis Submitted to
MAFSU, Nagpur. |
|
|
23. Barlett, P.C., Miller, G.Y., Anderson, C R. and Kirk, J.H.
(1990) Milk production and somatic cell count in Michigan
dairy herds. J. Dairy Sci., 73(10): 2794-2800.
http://dx.doi.org/10.3168/jds.S0022-0302(90)78966-7 |
|
|
24. Hallen, S.C., Waller, P. and Emanuelson, U. (2008)
Therapeutic effects of systemic or intramammary antimicrobial
treatment of bovine subclinical mastitis during lactation.
Vet. J., 175(1): 108-117.
http://dx.doi.org/10.1016/j.tvjl.2006.12.005
PMid:17320434 |
|
|
|
25. Argherie, D. (2008) The emphasize of some enzymatic
reactions in somatic cells from normal and abnormal milk.
Lucrari Stiintifice Med. Vet. Timisoara, 2008;12:504-509. |
|
|
|
