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R esearch
(Published online:
06-05-2015)
4.
Influenza A plasma and serum virus antibody detection comparison
in dogs using blocking enzyme-linked immunosorbent assay
-
H. T.
Lin, C. H. Hsu, H. J. Tsai, C. H. Lin, P. Y. Lo, S. L. Wang and L.
C. Wang
Veterinary World, 8(5): 580-583
doi:
10.14202/vetworld.2015.580-583
H. T. Lin:
Institute of Veterinary Clinical Sciences, School of Veterinary
Medicine, National Taiwan University, 1 Sec. 4, Roosevelt Road,
Taipei 10617, Taiwan;
drephy0961897@gmail.com
C. H. Hsu:
Institute of Veterinary Clinical Sciences, School of Veterinary
Medicine, National Taiwan University, 1 Sec. 4, Roosevelt Road,
Taipei 10617, Taiwan;
b01609074@ntu.edu.tw
H. J. Tsai:
Institute of Veterinary Clinical Sciences, School of Veterinary
Medicine, National Taiwan University, 1 Sec. 4, Roosevelt Road,
Taipei 10617, Taiwan;
hjjtsai@ms52.url.com.tw
C. H. Lin:
Institute of Veterinary Clinical Sciences, School of Veterinary
Medicine, National Taiwan University, 1 Sec. 4, Roosevelt Road,
Taipei 10617, Taiwan;
sail@pie.com.tw
P. Y. Lo:
Institute of Veterinary Clinical Sciences, School of Veterinary
Medicine, National Taiwan University, 1 Sec. 4, Roosevelt Road,
Taipei 10617, Taiwan;
pylo13@gmail.com
S. L. Wang:
Institute of Veterinary Clinical Sciences, School of Veterinary
Medicine, National Taiwan University, 1 Sec. 4, Roosevelt Road,
Taipei 10617, Taiwan;
b88609024@ntu.edu.tw
L. C. Wang: Institute of Veterinary
Clinical Sciences, School of Veterinary Medicine, National Taiwan
University, 1 Sec. 4, Roosevelt Road, Taipei 10617, Taiwan;
lcwang@ntu.edu.tw
Received:
8-11-2014, Revised: 31-03-2015, Accepted: 07-04-2015, Published
online: 06-05-2015
Corresponding author:
L. C. Wang, e-mail:
lcwang@ntu.edu.tw
Citation:
Lin
HT, Hsu CH, Tsai HJ, Lin CH, Lo PY, Wang SL, Wang LC (2015)
Influenza A plasma and serum virus antibody detection comparison
in dogs using blocking enzyme-linked immunosorbent assay (bELISA),
Veterinary World 8(5):580-583.
Abstract
Background and Aim: The
influenza A virus (IAV) is an important zoonotic pathogen with
infections also reported in dogs. IAV infections can be detected
through the presence of antibodies using the enzyme-linked
immunosorbent assay (ELISA). Serum is the only standard sample
source; however, there is no information on the availability of
other sample sources for IAV antibody detection in dogs. Compared
with serum, plasma is more widely employed in most animal
hospitals. The object of this study is to investigate whether
plasma collected in ethylenediaminetetraacetic acid (EDTA) tubes (EDTA
plasma) or heparin tubes (heparin plasma) could be used in the
ELISA protocol instead of serum for IAV antibody detection in
dogs.
Materials and Methods: Totally, 82 matched EDTA plasma and
serum sample pairs and 79 matched heparin plasma and serum sample
pairs were employed using blocking enzyme-linked immunosorbent
assay (bELISA). The agreement and correlation between the plasma (EDTA
or heparin plasma) and serum were assessed using the agreement
index kappa (kD) calculation and Pearson correlation
coefficient, respectively.
Results: The agreement index kD of EDTA plasma and
serum was 1.0, and that of heparin plasma and serum was 0.85. The
Pearson correlation coefficient of EDTA plasma and serum was 0.87
(p<0.01), and that of heparin plasma and serum was 0.82 (p<0.01).
Conclusion: The results proved that plasma, especially EDTA
plasma, could be substituted for serum in the bELISA test. This
might greatly expand the clinical applicability of IAV antibody
detection in dogs.
Keywords: dog, enzyme-linked immunosorbent assay, influenza A
virus, plasma, serum.
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