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R esearch
(Published online:
24-11-2015)
12.
Biochemical studies in experimentally
Escherichia coli
infected broiler chicken supplemented with neem (Azadirachta
indica)
leaf extract -
Vikash Sharma, K. K. Jakhar, Vikas Nehra and Sarvan Kumar
Veterinary World, 8(11): 1340-1345
doi:
10.14202/vetworld.2015.1340-1345
Vikash Sharma:
Department of Veterinary Pathology, Lala Lajpat Rai University
of Veterinary & Animal Sciences, Hisar, Haryana, India;
sharmavikashjind@gmail.com
K. K. Jakhar:
Department of Veterinary Pathology, Lala Lajpat Rai University
of Veterinary & Animal Sciences, Hisar, Haryana, India;
hod.vpp@luvas.edu.in
Vikas Nehra:
Department of Veterinary Pathology, Lala Lajpat Rai University
of Veterinary & Animal Sciences, Hisar, Haryana, India;
drvikasnehra@gmail.com
Sarvan Kumar:
Department of Veterinary Pathology, Lala Lajpat Rai University
of Veterinary & Animal Sciences, Hisar, Haryana, India;
drsrvn38@gmail.com
Received: 24-06-2015, Revised: 10-10-2015, Accepted: 17-10-2015,
Published online: 24-11-2015
Corresponding author:
Vikash Sharma, e-mail: sharmavikashjind@gmail.com
Citation:
Sharma V, Jakhar KK, Nehra V, Kumar S (2015) Biochemical studies
in experimentally
Escherichia coli
infected broiler chicken supplemented with neem (Azadira
chta
indica)
leaf extract,
Veterinary World 8(11):
1340-1345.
Abstract
Aim:
An experimental study was conducted on 192-day-old broiler
chicks for evaluating the effect of 10% neem leaf extract (NLE)
supplementationon biochemical parameters in chickens
experimentally infected with
Escherichia coli
O78 at 107 CFU/0.5 ml at 7 days of age.
Materials and Methods:
The 192-day-old broiler chicks were procured. These chicks were
divided into two groups (A and B) containing 96 birds each on
the 1st day. Diet of all the chicks of Group A was supplemented
with 10%NLE in water, whereas chicks of Group B were given feed
and water devoid of NLE supplementation throughout the
experiment. After rearing for 1 week, chicks of both the groups
(A and B) were again divided into two subgroups (Group A into A1
and A2 and Group B into B1 and B2) of 54 and 42 birds,
respectively. At the age of 7 days all the chicks of groups A1
and B1 were injected with
E. coli
O78 at 107 CFU/0.5 ml intraperitoneally. Blood samples were
collected from six chicks from each group at day 0, 2, 4, 7, 14,
21, 28 days post-infection and serum was separated for
biochemical studies.
Results:
There was a significant increase in serum alanine transaminase
(ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH)
activities, globulin concentration and a decrease in total
protein (TP), albumin concentrations, and alkaline phosphatase
(ALP) activity in both the infected groups. However, the changes
in biochemical values, i.e., ALT, AST, LDH, ALP, TP, albumin,
and globulin wereof lower magnitude in NLE supplemented group
suggesting hepatoprotective and cardioprotective effect of NLE.
Conclusions:
Fromthe present study, it is reasonable to conclude that
significant increase in the value of ALT, AST, LDH, globulin,
and significant decrease in the value of ALP, TP, and albumin
was of lower magnitude in supplemented infected group (A1) as
compared to non-supplemented infected group (B1) suggesting
hepatoprotective and cardioprotective effect of NLE.
Keywords:
biochemical studies, chicken, experimentally
Escherichia coli
infected broiler, neem leaf extract.
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