Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
R esearch
(Published online:
28-11-2015)
17.
Comparison of molecular and
microscopic technique for detection of Theileria annulata
from the field cases of cattle
-
H. C. Chauhan, B. K. Patel, A. G. Bhagat, M. V. Patel, S. I.
Patel, S. H. Raval, H. H. Panchasara, M. D. Shrimali, A. C.
Patel
and B. S. Chandel
Veterinary World, 8(11): 1370-1374
doi:
10.14202/vetworld.2015.1370-1374
H. C. Chauhan:
Division of Animal
Biotechnology, College of Veterinary Science & Animal Husbandry,
Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar - 385 506, Gujarat, India;
hcchauhan1972@gmail.com
B. K. Patel:
Division of
Animal Biotechnology, College of Veterinary Science & Animal
Husbandry, Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar - 385 506, Gujarat, India;
bharatpatel1063@yahoo.com
A. G. Bhagat:
Division of
Veterinary Microbiology, College of Veterinary Science & Animal
Husbandry, Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar - 385 506, Gujarat, India;
abidalibhagat@gmail.com
M. V. Patel:
Division of
Veterinary Microbiology, College of Veterinary Science & Animal
Husbandry, Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar - 385 506, Gujarat, India;
patelmv960@gmail.com
S. I. Patel:
Division of
Veterinary Microbiology, College of Veterinary Science & Animal
Husbandry, Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar - 385 506, Gujarat, India;
dr.sanjivpatel8790@gmail.com
S. H. Raval:
Division of
Veterinary Pathology, College of Veterinary Science & Animal
House, Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar - 385 506, Gujarat, India;
samirraval81@gmail.com
H. H. Panchasara:
Teaching
Veterinary Clinical Complex (College Clinics), College of
Veterinary Science & Animal House, Sardarkrushinagar Dantiwada
Agricultural University, Sardarkrushinagar - 385 506, Gujarat,
India;
harshadlrs@yahoo.co.in
M. D. Shrimali:
Division of
Animal Biotechnology, College of Veterinary Science & Animal
Husbandry, Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar - 385 506, Gujarat, India;
shrimali.vets@gmail.com
A. C. Patel :
Division of
Veterinary Microbiology, College of Veterinary Science & Animal
Husbandry, Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar - 385 506, Gujarat, India;
viroarun@gmail.com
B. S. Chandel:
Division of
Animal Biotechnology, College of Veterinary Science & Animal
Husbandry, Sardarkrushinagar Dantiwada Agricultural University,
Sardarkrushinagar - 385 506, Gujarat, India;
bschandel13@gmail.com
Received: 23-06-2015, Revised: 08-10-2015, Accepted: 19-10-2015,
Published online: 28-11-2015
Corresponding author:
A. C. Patel, e-mail: viroarun@gmail.com
Citation:
Chauhan HC, Patel BK, Bhagat AG, Patel MV, Patel SI, Raval SH,
Panchasara HH, Shrimali MD, Patel AC, Chandel BS (2015)
Comparison of molecular and microscopic technique for detection
of Theileria annulata from the field cases of cattle,
Veterinary World 8(11): 1370-1374.
Abstract
Aim:
Tropical
theileriosis is fatal hemoprotozoal disease of dairy animals
caused by Theileria annulata. The aim of the present
study was to detect the T. annulata and comparison of
results of molecular and microscopic techniques.
Materials and
Methods:
A total of 52
blood samples were collected from the cattle suspected for
theileriosis across the Banaskantha district. All the samples
were screened for theileriosis using Giemsa’s staining technique
and polymerase chain reaction (PCR).
Results:
Total of 17
(32.69%) and 24 (46.15%) samples were found positive for
theileriosis by microscopic examination and PCR test,
respectively. It revealed that the study area is endemic for
theileriosis, and the microscopic technique has 70.83%
sensitivity and 100% specificity with respect to PCR technique.
Conclusion:
It may
be concluded from the present study that the PCR is
comparatively sensitive technique than microscopic examination
and may be recommended to use in the field for screening of
theileriosis in the study area, where a high prevalence of
diseases have been reported due to intensive dairy farming.
Keywords:
Giemsa staining, polymerase chain reaction, theileriosis.
References
1. Omer, O.H., El-Malik, K.H., Mahmoud, O.M., Haroun, E.M.,
Hawas, A., Sweeney, D. and Magzoub, M. (2002) Hematological
profiles in pure bred cattle naturally infected with
Theileria annulata in Saudi Arabia. Vet. Parasitol.,
107(1-2): 161-168.
http://dx.doi.org/10.1016/S0304-4017(02)00094-8 |
|
|
2. El-Deeb, W.M. and Younis, E.E. (2009) Clinical and
biochemical studies on Theileria annulata in Egyptian
buffaloes (Bubalus bubalis) with particular orientation to
oxidative stress and ketosis relationship. Vet. Parasitol.,
164(2-4): 301-305.
http://dx.doi.org/10.1016/j.vetpar.2009.06.002
PMid:19559534 |
|
|
3. Uilenberg, G. (1981) Theileria species of domestic
livestock. In: Irvin, A.D., Cunningham, M.P. and Young, A.S.,
editors. Advances in the Control of Theileriosis. Martinus
Nijhoff, The Hague. p4-37.
http://dx.doi.org/10.1007/978-94-009-8346-5_2 |
|
|
|
4. Available from: http://www.nddb.org/AnnualReports/nddb-annual-report-2011-2012.pdf
. Accessed on 30-04-2015. |
|
|
|
5. Available from: http://www.dahd.nic.in/dahd/WriteReadData/Livestock.2012.pdf.
Accessed on 24-04-2015. |
|
|
|
6. Taylor, M.A., Coop, R.L. and Wall, R.L. (2007) Veterinary
Parasitology. 3rd ed. Blackwell Publishing, Oxford. |
|
|
7. Nourollahi-Fard, S.R., Khalili, M. and Ghalekhani, N.
(2012) Detection of Theileria annulata in blood samples of
native cattle by PCR and smear method in Southeast of Iran.
J. Parasitol. Dis., 39(2): 249-252.
http://dx.doi.org/10.1007/s12639-013-0333-2
PMid:26064010 |
|
|
8. Aktas, M., Altay, K. and Dumanli, N. (2006) A molecular
survey of bovine Theileria parasites among apparently
healthy cattle and with a note on the distribution of ticks
in eastern Turkey. Vet. Parasitol., 138(3-4): 179-185.
http://dx.doi.org/10.1016/j.vetpar.2006.01.052
PMid:16510248 |
|
|
9. OIE. (2014) Manual of Diagnostic Tests and Vaccines for
Terrestrial Animals. 7th ed., Vol. 1-2. Office International
Des Epizooties, Paris.
PMCid:PMC4318122 |
|
|
10. Pipano, E. and Cahana, M. (1969) Fluorescent antibody
test for the serodiagnosis of Theileria annulata. J.
Parasitol., 55(4): 765.
http://dx.doi.org/10.2307/3277214 |
|
|
11. Burridge, M.J., Brown, C.G.D. and Kimber, C.D. (1974)
Theileria annulata: Cross reactions between a cell culture
schizont antigen and antigens of East African Theileria
species in the indirect fluorescent antibody test. Exp.
Parasitol., 35: 374-380.
http://dx.doi.org/10.1016/0014-4894(74)90043-5 |
|
|
12. Friedhoff, K. and Bose, R. (1994) Recent developments in
diagnostics of some tick-borne diseases. In: Uilenberg, G.,
Permin, A. and Hansen, J.W., editors. Use of Applicable
Biotechnological Methods for Diagnosing Haemoparasites.
Proceedings of the Expert Consultation, Merida, Mexico, 4-6
October 1993. Food and Agriculture Organisation of the
United Nations (FAO), Rome, Italy. p46-57.
PMCid:PMC523719 |
|
|
13. Altay, K., Aydin, M.F., Dumanli, N. and Aktas, M. (2008)
Molecular detection of Theileria and Babesia infections in
cattle. Vet. Parasitol., 158(4): 295-301.
http://dx.doi.org/10.1016/j.vetpar.2008.09.025
PMid:19008048 |
|
|
14. Kundave, V.R., Patel, A.K., Patel, P.V., Hasnani, J.J.
and Joshi, C.G. (2014) Qualitative and quantitative
assessment of Theileria annulata in cattle and buffaloes
polymerase chain reaction. Trop. Biomed., 31(4): 728-735.
PMid:25776598 |
|
|
15. Noaman, V. (2014) Comparison of molecular and
microscopic technique for detection of Theileria spp. in
carrier cattle. J. Parasit. Dis., 38(1): 64-67. Doi:
10.1007/s12639-012-0196-y.
http://dx.doi.org/10.1007/s12639-012-0196-y |
|
|
16. Dumanli, N., Aktas, M., Cetinkaya, B., Cakmak, A.,
Koroglu, E., Saki, C.E., Erdogmus, Z., Nalbantoglu, S.,
Ongor, H., Simsek, S., Karahan, M. and Altay, K. (2005)
Prevalence and distribution of tropical theileriosis in
eastern Turkey. Vet. Parasitol., 127(1): 9-15.
http://dx.doi.org/10.1016/j.vetpar.2004.08.006
PMid:15619369 |
|
|
17. Azizi, H., Shiran, B., Farzaneh, D.A., Salehi, F. and
Taghadosi, C. (2008) Detection of Theileria annulata by PCR
and its comparison with smear method in native carrier cows.
Biotechnology, 79(3): 574-577.
http://dx.doi.org/10.3923/biotech.2008.574.577 |
|
|
18. Nayel, M,, El-Dakhly, K,M., Aboulaila, M., Elsify, A.,
Hassan, H., Ibrahim, E., Salama, A. and Yanai, T. (2012) The
use of different diagnostic tools for Babesia and Theileria
parasites in cattle in Menoufia, Egypt. Parasitol. Res.,
111(3):1019-1024. Doi: 10.1007/s00436-012-2926-6.
http://dx.doi.org/10.1007/s00436-012-2926-6 |
|
|
|
19. Hoghooghi-Rad, N., Ghaemi, P. and Shayan, P. (2011)
Detection of native carrier cattle infected with Theileria
annulata by seminested PCR and smear method in Golestan
province of Iran. World Appl. Sci. J., 12(3): 317-323. |
|
|
20. Kohli, S., Atheya, U.K. and Thapliyal, A. (2014)
Prevalence of theileriosis in cross-bred cattle: Its
detection through blood smear examination and polymerase
chain reaction in Dehradun district, Uttarakhand, India.
Vet. World, 7(3): 168-171.
http://dx.doi.org/10.14202/vetworld.2014.168-171 |
|
|
21. Shayan, P. and Rahbari, S. (2005) Simultaneous
differentiation between Theileria sp. and Babesia sp. on
stained blood smear using PCR. Parasitol. Res., 97(4):
281-286.
http://dx.doi.org/10.1007/s00436-005-1434-3
PMid:16007464 |
|
|
22. Roy, K.C., Ray, D., Bansal, G.C. and Singh, R.K. (2000)
Detection of Theileria annulata carrier cattle by PCR.
Indian J. Exp. Biol., 38: 283-284.
PMid:10927875 |
|
