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R esearch
(Published online:
22-11-2015)
9.
Staining of Platyhelminthes by herbal
dyes: An eco-friendly technique for the taxonomist -
Niranjan Kumar, Jadav Mehul, Bhupamani Das and J. B. Solanki
Veterinary World, 8(11): 1321-1325
doi:
10.14202/vetworld.2015.1321-1325
Niranjan Kumar:
Department of Parasitology, Vanbandhu Veterinary College,
Navsari Agricultural University, Navsari - 396 450, Gujarat,
India;
niruvet@gmail.com
Jadav Mehul:
Department of Parasitology, Vanbandhu Veterinary College,
Navsari Agricultural University, Navsari - 396 450, Gujarat,
India;
jadavvety@gmail.com
Bhupamani Das:
Department of Parasitology, Vanbandhu Veterinary College,
Navsari Agricultural University, Navsari - 396 450, Gujarat,
India;
bhupa67@gmail.com
J. B. Solanki:
Department of Parasitology, Vanbandhu Veterinary College,
Navsari Agricultural University, Navsari - 396 450, Gujarat,
India;
jbsolankivet@gmail.com
Received: 30-05-2015, Revised: 30-09-2015, Accepted: 10-10-2015,
Published online: 22-11-2015
Corresponding author:
Niranjan Kumar, e-mail: niruvet@gmail.com
Citation:
Kumar N, Mehul J, Das B, Solanki JB (2015) Staining of
Platyhelminthes by herbal dyes: An eco-friendly technique for
the taxonomist,
Veterinary World 8(11):
1321-1325.
Abstract
Aim:
An environment compatible technique to stain Platyhelminthes,
Fasciola gigantica,
Gastrothylax crumenifer,
Taenia solium,
and
Moniezia expansa
using aqueous and alcoholic extract of sugar beet (Beta
vulgaris),
China rose (Hibiscus
rosasinensis),
and red rose (Rosa
hybrida)
were described to minimized the deleterious effects of the
synthetic dyes.
Materials and Methods:
Aqueous/ethanolic extracts of roses were extracted from the
flowers while red beet was extracted from the roots.
Results:
Stained helminthes acquired a comparable level of pigmentation
with the distinction of their internal structure in these
natural dyes. The flukes (liver and rumen) internal structure,
oral and ventral/posterior sucker, cirrus sac, gravid uterus,
testes, ovary, and vitallaria were appeared pink color in
aqueous and alcoholic extract of either China or red rose and
yellow to brown color in sugar beet stain. The interior of the
proglottid of
T. solium
and
M. expansa
took yellow to brown color with good contrast in sugar beet
stain and of pink to pink-red in China and red rose stain.
Conclusion:
The extract of roses (red rose followed by China rose) followed
by red beet possess the potential to replace the conventional
stains in the taxonomic study of Platyhelminthes parasites.
Keywords:
herbal dyes, Platyhelminthes, rose, sugar beet.
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