Open Access
Research
(Published
online: 28-04-2016)
14.
In vitro
larvicidal effects of ethanolic extract of Curcuma longa
Linn. on Haemonchus larval stage -
Norisal Binti Nasai,
Yusuf Abba,
Faez Firdaus Jesse Abdullah,
Murugaiyah Marimuthu,
Abdulnasir Tijjani, Muhammad Abubakar Sadiq,
Konto Mohammed,
Eric Lim Teik Chung
and Mohammed Ariff Bin Omar
Veterinary World, 9(4): 417-420
doi:
10.14202/vetworld.2016.417-420
Norisal Binti Nasai :
Department of
Veterinary Clinical Studies, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor,
Malaysia; usuba5050@live.com
Yusuf Abba :
Department of
Veterinary Pathology and Microbiology, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor,
Malaysia; yabbavet@gmail.com
Faez Firdaus Jesse Abdullah:
Department of
Veterinary Clinical Studies, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor,
Malaysia; Research Centre for Ruminant Disease, Faculty of
Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM
Serdang, Selangor, Malaysia; jesseariasamy@gmail.com
Murugaiyah Marimuthu:
Department of
Veterinary Clinical Studies, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor,
Malaysia; Department of Veterinary Pathology and Microbiology,
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400
UPM Serdang, Selangor, Malaysia; researchofficer2002@yahoo.com
Abdulnasir Tijjani :
Department of
Veterinary Pathology and Microbiology, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor,
Malaysia; nasirvet69@gmail.com
Muhammad Abubakar Sadiq :
Department of
Veterinary Pathology and Microbiology, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor,
Malaysia; masadiqvet@gmail.com
Konto Mohammed :
Department of
Veterinary Clinical Studies, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor,
Malaysia; kontomohammed@gmail.com
Eric Lim Teik Chung :
Department of
Veterinary Clinical Studies, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor,
Malaysia; ericlim88@gmail.com
Mohammed Ariff Bin Omar:
Department of
Veterinary Clinical Studies, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor,
Malaysia; Research Centre for Ruminant Disease, Faculty of
Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM
Serdang, Selangor, Malaysia; mo_ariff@upm.edu.my
Received: 20-10-2015, Accepted: 16-03-2016, Published online:
28-04-2016
Corresponding author:
Faez Firdaus Jesse Abdullah, e-mail: jesseariasamy@gmail.com
Citation:
Nasai NB, Abba Y, Abdullah FFJ, Marimuthu M, Tijjani A, Sadiq
MA, Mohammed K, Chung ELT, Omar MAB (2016) In vitro
larvicidal effects of ethanolic extract of Curcuma longa
Linn. on Haemonchus larval stage, Veterinary World, 9(4):
417-420.
Abstract
Aim:
Gastrointestinal helminthosis is a global problem in small
ruminant production. Most parasites have developed resistance to
commonly available anthelminthic compounds, and there is
currently an increasing need for new compounds with more
efficacies. This study evaluated the in vitro effects of
ethanolic extract of Curcuma longa (EECL) as a biological
nematicide against third stage Haemonchus larvae (L3)
isolated from sheep.
Materials and
Methods:
Haemonchus
L3
were cultured and harvested from the feces of naturally infected
sheep. EECL was prepared and three concentrations; 50, 100, and
200 mg/mL were tested for their efficacies on Haemonchus
L3. Levamisole at concentration 1.5 and 3 mg/mL were used as
positive controls.
Results:
EECL
showed anthelmintic activity in a dose-dependent manner with 78%
worm mortality within 24 h of exposure at the highest dose rate
of 200 mg/mL. There was a 100% worm mortality rate after 2 h of
levamisole (3 mg/mL) admisntration. However, there was a
comparable larvicidal effect between when levamisole (1.5 mg/mL)
and EECL (200 mg) were administered.
Conclusion:
The
study shows that EECL does exhibit good anthelmintic properties
at 200 mg/mL which is comparable with levamisole at 1.5 mg/mL.
Keywords:
Curcuma longa,
ethanolic extract, Haemonchus, larvae 3, levamisole,
strongyle.
References
|
1. Ullah, S., Khan, M.N., Sajid, M.S. and Muhammad, G.
(2013) Comparative anthelmintic efficacy of Curcuma longa,
Citrullus colocynthis and Peganum harmala. Glob. Vet.,
11(5): 560-567. |
|
|
|
2. Akram, M., Shahab-Uddin, A.A., Usmanghani, K., Hannan,
A., Mohiuddin, E. and Asif, M. (2010) Curcuma longa and
Curcumin: A review article. Rom. J. Plant Biol., 55(2):
65-70. |
|
|
|
3. Velayudhan, K.C., Dikshi, N. and Nizar, M.A. (2012)
Ethnobotany of turmeric (Curcuma longa L.). Indian J. Tradit.
Knowl., 11(4): 607-614. |
|
|
4. Shahiduzzaman, M. and Daugschies, A. (2011) Curcumin: A
natural herb extract with antiparasitic properties. In:
Nature Helps. Springer, Berlin Heidelberg. p141-152.
http://dx.doi.org/10.1007/978-3-642-19382-8_6 |
|
|
|
5. Vidya, J., Kale, R.D. and Nair, P. (2012) Earthworm
muscle cells as models to study anthelminitic properties of
plant extracts. Int. J. Res. Pharm. Biomed. Sci., 3(2):
489-496. |
|
|
6. Magalhães, L.G., Machado, C.B., Morais, E.R., De
Carvalho, M.É.B., Soares, C.S., Da Silva, S.H. and
Rodrigues, V. (2009) In vitro schistosomicidal activity of
curcumin against Schistosoma mansoni adult worms. Parasitol.
Res., 104(5): 1197-1201.
http://dx.doi.org/10.1007/s00436-008-1311-y
PMid:19096877 |
|
|
7. Bazh, E.K. and El-Bahy, N.M. (2013) In vitro and in vivo
screening of anthelmintic activity of ginger and curcumin on
Ascaridia galli. Parasitol. Res., 112(11): 3679-3686.
http://dx.doi.org/10.1007/s00436-013-3541-x
PMid:24046262 |
|
|
8. Salama, S.M., Abdulla, M.A., AlRashdi, A.S., Ismail, S.,
Alkiyumi, S.S. and Golbabapour, S. (2013) Hepatoprotective
effect of ethanolic extract of Curcuma longa on
thioacetamide induced liver cirrhosis in rats. BMC Compl.
Altern. Med., 13(1): 56.
http://dx.doi.org/10.1186/1472-6882-13-56 |
|
|
|
9. Hansen, J.W. and Perry, B.D. (1994) Techniques for
parasite assays and identification in faecal samples. The
Epidemiology, Diagnosis and Control of Helminth Parasites of
Ruminants. International Laboratory for Research on Animal
Diseases, Nairobi, Kenya. |
|
|
|
10. Singh, R., Mehta, A. and Shukla, K. (2011) Anthelmintic
activity of rhizome extracts of Curcuma longa and Zingiber
officinale (Zingiberaceae). Int. J. Pharm. Pharm. Sci.,
3(2): 236-237. |
|
|
|
11. Kulkarni, S.J., Maske, K.N., Budre, M.P. and Mahajan,
R.P. (2012) Extraction and purification of curcuminoids from
turmeric (Curcuma longa L.). Int. J. Pharmacol. Pharm.
Technol., 1(2): 29-34. |
|
|
12. Morais, E.R., Oliveira, K.C., Magalhães, L.G., Moreira,
É.B., Verjovski-Almeida, S. and Rodrigues, V. (2013) Effects
of curcumin on the parasite Schistosoma mansoni: A
transcriptomic approach. Mol. Biochem. Parasitol., 187(2):
91-97.
http://dx.doi.org/10.1016/j.molbiopara.2012.11.006
PMid:23276630 |
|
|
13. Mohammed, K., Abba, Y., Ramli, N. S. B., Marimuthu, M.,
Omar, M. A., Abdullah, F. F. J., Sadiq, M.A., Tijjani, A.,
Chung, E.L.T. and Lila, M. A. M. (2016). The use of FAMACHA
in estimation of gastrointestinal nematodes and total worm
burden in Damara and Barbados Blackbelly cross sheep. Trop.
Anim. Health Prod., 1-8
http://dx.doi.org/10.1007/s11250-016-1049-y |
|
|
14. Martin, R.J. (1997) Review: Modes of action of
anthelmintic drugs. Vet. J., 154: 11-34.
http://dx.doi.org/10.1016/S1090-0233(05)80005-X |
|
|
15. Martin, R.J. and Robertson, A.P. (2007) Mode of action
of levamisole and pyrantel, anthelmintic resistance, E153
and Q57. Parasitology, 134: 1093-1104.
http://dx.doi.org/10.1017/S0031182007000029
PMid:17608969 |
|
