Open Access
Research
(Published
online: 16-08-2016)
11.
Evaluation of complementary
diagnostic tools for bovine tuberculosis detection in dairy
herds from India -
Mukesh Kumar Thakur,
Dharmender Kumar Sinha
and Bhoj Raj Singh
Veterinary World, 9(8): 862-868
doi:
10.14202/vetworld.2016.862-868
Mukesh Kumar
Thakur :
School of Public
Health & Zoonoses, Guru Angad Dev Veterinary & Animal Sciences
University, Ludhiana, Punjab, India;
vetsamu@gmail.com
Dharmender Kumar
Sinha :
Division of
Epidemiology, Indian Veterinary Research Institute, Izatnagar,
Bareilly, Uttar Pradesh, India;
sinhaivri@rediffmail.com
Bhoj Raj Singh :
Division of
Epidemiology, Indian Veterinary Research Institute, Izatnagar,
Bareilly, Uttar Pradesh, India; brs1762@gmail.com
Received: 29-04-2016, Accepted: 08-07-2016, Published online:
16-08-2016
Corresponding author:
Mukesh
Kumar Thakur, e-mail: vetsamu@gmail.com
Citation:
Thakur MK, Sinha DK, Singh BR (2016) Evaluation of complementary
diagnostic tools for bovine tuberculosis detection in dairy
herds from India, Veterinary World, 9(8): 862-868.
Abstract
Aim:
A
cross-sectional study was undertaken to know the herd prevalence
and evaluate the single intradermal tuberculin testing (SITT),
culture isolation, and polymerase chain reaction (PCR) analysis
for the diagnosis of bovine tuberculosis (TB).
Materials and
Methods:
A total of 541
cows of three dairy farms of Bareilly and Mukteshwar were
screened by SITT followed by collection of pre-scapular lymph
node (PSLN) aspirates (71), milk (54), and blood (71) samples
from reactor animals. These clinical samples were processed for
culture isolation and direct PCR-based identification and
species differentiation.
Results:
Out
of 541 cows screened by SITT, 71 (13.12%) animals were found
positive. Mycobacteria were isolated from 3 (4.22%) PSLN
aspirate but not from any cultured milk and blood samples. 28
(39.43%) PSLN aspirate and 5 (9.25%) milk samples were positive
for Mycobacterium TB (MTB) complex (MTC) by PCR
amplification for the IS6110 insertion sequence; however,
blood samples were found negative. For species differentiation,
multiplex-PCR using 12.7 kb primers was conducted. Out of 28
PSLN aspirate, Mycobacterium bovis was detected in 18
(64.28%) and MTB in 8 (28.57%), whereas 2 aspirate samples
(7.14%) were positive for both the species. All the five milk
positive samples were positive for M. bovis.
Conclusion:
Direct detection of bovine TB by a molecular-based method in
dairy animals after preliminary screening was appeared to be
more sensitive and specific compared to the conventional method
(i.e., culture isolation). Its application in form of serial
testing methodology for the routine diagnosis and thereafter,
culling of infected stock may be suggested for the control
programs in dairy herds. The PSLN aspirate was found to be the
most suitable specimen for culture isolation and PCR-based
detection of Mycobacterium spp. among live infected
animals.
Keywords:
bovine tuberculosis, culture isolation, Mycobacterium
tuberculosis complex, polymerase chain reaction, single
intradermal tuberculin testing.
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