Open Access
Research
(Published
online: 29-08-2016)
20.
A study on association of virulence determinants of verotoxic
Escherichia coli
isolated from cattle calves -
Singh Parul, Basanti Bist, Barkha Sharma, Udit Jain and Janardan
K. Yadav
Veterinary World, 9(8): 915-918
doi:
10.14202/vetworld.2016.915-918
Singh Parul:
State Veterinary Hospital, Dhanateja, Mathura, Uttar Pradesh,
India;
parulkaler@rediffmail.com
Basanti Bist:
Department of Veterinary Public
Health, College of Veterinary Sciences
and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Veterinary
Science University, Mathura, Uttar Pradesh, India; vasanti.bist@gmail.com
Barkha Sharma:
Department of Epidemiology and Preventive Veterinary Medicine,
College of Veterinary Sciences and Animal Husbandry, UP Pandit
Deen Dayal Upadhyaya Veterinary Science University, Mathura,
Uttar Pradesh, India; manubarkha@yahoo.com
Udit Jain:
Department of Veterinary Public
Health, College of Veterinary Sciences
and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Veterinary
Science University, Mathura, Uttar Pradesh, India; druditjain@hotmail.com
Janardan K. Yadav:
Department of Veterinary Public
Health, College of Veterinary Sciences
and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Veterinary
Science University, Mathura, Uttar Pradesh, India;
janardanyadav1987@gmail.com
Received: 06-05-2016, Accepted: 25-07-2016, Published online:
29-08-2016
Corresponding author:
Singh Parul, e-mail: parulkaler@rediffmail.com
Citation:
Parul S, Bist B, Sharma B, Jain U, Yadav JK (2016) A study on
association of virulence determinants of verotoxic
Escherichia coli
isolated from cattle calves,
Veterinary World, 9(8):
915-918.
Abstract
Aim:
The present study was conducted to find the association among
virulence determinants of verotoxic
Escherichia coli
(VTEC) isolated from cattle calf feces.
Materials and Methods:
A total of 216 cattle calf fecal samples were collected
aseptically and processed under required conditions for the
isolation of
E. coli.
The isolates were further subjected to multiplex polymerase
chain reaction (mPCR) for the detection of virulent genes. All
the VTEC isolates were serotyped at the Central Research
Institute, Kasauli, Himachal Pradesh. The VTEC isolates were
observed for the enterohemolysin production on washed sheep
blood agar (wSBA).
Results:
A total of 177 presumptive
E. coli
were isolated from 216 calf fecal samples revealing an overall
prevalence of
E. coli
to be 81.94%. A total of 32 (14.81%) isolates were detected as
VTEC through mPCR. The prevalence of verotoxin genes
vt1,
vt2,
and combination of
vt1+vt2
in the VTEC isolates was found to be 12 (37.5%), 14 (43.75%),
and 6 (18.75%), respectively. Other virulent genes
eaeA
and
hlyA
were found in 6 and 11 VTEC strains with prevalence values of
18.75% and 34.37%, respectively. A total of 13 different O
serogroups were revealed in serotyping of 32 VTEC isolates. Out
of 32 VTEC strains, only 26 (81.25%) were enterohemolytic on
wSBA as they produced the characteristic small, turbid zone of
hemolysis around the streaking line. Although enterohemolysin
production has been attributed to the presence of
hlyA
gene, only 11 of 26 enterohemolysin producing VTEC were found to
be harboring the
hlyA
gene (11/26) 42.03%.
Conclusion:
The present study concludes that there might be an association
between the presence of verotoxin genes and enterohemolysin
production in VTEC group of
E. coli.
Keywords:
enterohemolysin gene (hlyA),
enterohemolysin toxin, intimin gene (eaeA),
verotoxic
Escherichia coli,
verotoxin genes (vt1
and
vt2),
virulence determinants.
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