Open Access
Research
(Published
online: 15-12-2016)
14.
Molecular characterization of field
infectious bursal disease virus isolates from Nigeria -
Ijeoma O. Nwagbo, Ismaila Shittu, Chika I. Nwosuh, George O.
Ezeifeka, Frederick J. C. Odibo, Linda O. Michel and Daral J.
Jackwood
Veterinary World, 9(12): 1420-1428
doi:
10.14202/vetworld.2016.1420-1428
Ijeoma O. Nwagbo:
Department of Virology, Viral Research Division, National
Veterinary Research Institute, Vom, Plateau State, Nigeria;
Department of Applied Microbiology and Brewing, Faculty of
Biosciences, Nnamdi Azikiwe University Awka, Anambra State,
Nigeria; Department of Veterinary Preventive Medicine, Food
Animal Health Research Program, Ohio Agricultural Research and
Development Center, The Ohio State University, 1680 Madison
Avenue, Wooster, OH 44691, USA; aijay02@yahoo.com
Ismaila Shittu:
Department of Virology, Viral Research Division, National
Veterinary Research Institute, Vom, Plateau State, Nigeria;
ismaila.shittu@gmail.com
Chika I. Nwosuh:
Department of Virology, Viral Research Division, National
Veterinary Research Institute, Vom, Plateau State, Nigeria;
chikanwosuh@nvri.gov.ng
George O. Ezeifeka:
Department of Veterinary Microbiology and Parasitology, College of
Veterinary Medicine, Micheal Okpara University of Agriculture
Umudike, Abia State, Nigeria; ezeifekag@yahoo.com
Frederick J. C. Odibo:
Department of Applied Microbiology and Brewing, Faculty of
Biosciences, Nnamdi Azikiwe University Awka, Anambra State,
Nigeria; profodibo@yahoo.com
Linda O. Michel:
Department of Veterinary Preventive Medicine, Food Animal Health
Research Program, Ohio Agricultural Research and Development
Center, The Ohio State University, 1680 Madison Avenue, Wooster,
OH 44691, USA; michel.37@osu.edu
Daral J. Jackwood:
Department of Veterinary Preventive Medicine, Food Animal Health
Research Program, Ohio Agricultural Research and Development
Center, The Ohio State University, 1680 Madison Avenue, Wooster,
OH 44691, USA; Jackwood.2@osu.edu
Received: 19-05-2016, Accepted: 15-11-2016, Published online:
15-12-2016
Corresponding author:
Ijeoma O. Nwagbo, e-mail: aijay02@yahoo.com
Citation:
Nwagbo IO, Shittu I, Nwosuh CI, Ezeifeka GO, Odibo FJC, Michel LO,
Jackwood DJ (2016) Molecular characterization of field infectious
bursal disease virus isolates from Nigeria,
Veterinary World, 9(12):
1420-1428.
Abstract
Aim:
To characterize field isolates of infectious bursal disease virus
(IBDV) from outbreaks in nine states in Nigeria through reverse
transcription polymerase chain reaction (RT-PCR) and sequence
analysis of portions of the VP2 and VP1 genes and to determine the
presence or absence of reassortant viruses.
Materials and Methods:
A
total of 377 bursa samples were collected from 201 suspected IBD
outbreaks during 2009 to 2014 from nine states in Nigeria. Samples
were subjected to RT-PCR using VP2 and VP1 gene specific primers,
and the resulting PCR products were sequenced.
Results:
A
total of 143 samples were positive for IBDV by RT-PCR. These
assays amplified a 743 bp fragment from nt 701 to 1444 in the IBDV
VP2 hypervariable region (hvVP2) of segment A and a 722 bp
fragment from nt 168 to 889 in the VP1 gene of segment B. RT-PCR
products were sequenced, aligned and compared with reference IBDV
sequences obtained from GenBank. All but one hvVP2 sequence showed
similarity to very virulent IBDV (vvIBDV) reference strains, yet
only 3 of the VP1 67 VP1 sequences showed similarity to the VP1
gene of vvIBDV. Phylogenetic analysis revealed a new lineage of
Nigerian reassortant IBDV strains.
Conclusion:
Phylogenetic analysis of partial sequences of genome segment A and
B of IBDV in Nigeria confirmed the existence of vvIBDV in Nigeria.
In addition, we noted the existence of reassortant IBDV strains
with novel triplet amino acid motifs at positions 145, 146 and 147
in the reassorted Nigerian IBDV.
Keywords:
infectious bursal disease virus, Nigeria, novel, reassortant, very
virulent.
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