| 
              
              
              Open Access  
 
              
              
              
              Research 
              
              
(Published 
				online: 
				16-02-2016)  
              10. 
				
				
				Utilization of carrageenan, citric acid 
				and cinnamon oil as an edible coating of chicken fillets to 
				prolong its shelf life under refrigeration conditions -
				
				
				Anshul Kumar Khare, Robinson J. J. Abraham, V. Appa Rao and R. 
				Narendra Babu 
              
              Veterinary World, 9(2): 166-175   
              
   
                
                
doi: 
              
				
				10.14202/vetworld.2016.166-175 
                
				  
				
				Anshul Kumar Khare: 
				
				Department of Livestock Products Technology (Meat Science), 
				Madras Veterinary College, Tamil Nadu Veterinary and 
				
				Animal Sciences University, Chennai - 600 007, Tamil Nadu, 
				India; akksagar@gmail.com 
				
				Robinson J. J. Abraham: 
				
				Department of Livestock Products Technology (Meat Science), 
				Madras Veterinary College, Tamil Nadu Veterinary and Animal 
				Sciences University, Chennai - 600 007, Tamil Nadu, India; 
				robinson@tanuvas.org.in 
				
				V. Appa Rao: 
				
				Department of Livestock Products Technology (Meat Science), 
				Madras Veterinary College, Tamil Nadu Veterinary and Animal 
				Sciences University, Chennai - 600 007, Tamil Nadu, India; 
				varao1966@yahoo.com 
				
				R. Narendra Babu: 
				
				Department of Livestock Products Technology (Meat Science), 
				Madras Veterinary College, Tamil Nadu Veterinary and Animal 
				Sciences University, Chennai - 600 007, Tamil Nadu, India; nbabu@tanuvas.org.in   
				
				Received: 01-08-2015, Revised: 26-12-2015, Accepted: 31-12-2015, 
				Published online: 16-02-2016 
				  
				
              	
              	Corresponding author:Anshul Kumar Khare, e-mail: akksagar@gmail.com 
 
              Citation: 
				
				Khare AK, Abraham RJJ, Rao VA, Babu RN (2016) Utilization of 
				carrageenan, citric acid, and cinnamon oil as an edible coating 
				of chicken fillets to prolong its shelf life under refrigeration 
				conditions, 
				
				Veterinary World 9(2): 
				166-175. 
 
              
				Abstract 
 
				
				
				Aim: 
				
				The present study was conducted to determine efficacy of edible 
				coating of carrageenan and cinnamon oil to enhance the shelf 
				life of chicken meat stored under refrigeration conditions. 
				
				
				Materials and Methods: 
				
				Chicken breast was coated with carrageenan and cinnamon oil by 
				three methods of application 
				
				viz., 
				spraying brushing and dipping. The coated meat was evaluated for 
				drip loss, pH, thiobarbituric acid number (TBA), tyrosine value 
				(TV)extract release volume (ERV), Warner-Bratzler shear force 
				value (WBSFV), instrumental color, microbiological, and sensory 
				qualities as per standard procedures. 
				
				
				Results: 
				
				There was a significant difference observed for physicochemical 
				parameters (pH, TBA, TV, ERV, drip loss and WBSFV) and 
				microbiological analysis between storage periods in all the 
				samples and between the control and treatments throughout the 
				storage period but samples did not differed significantly for 
				hunter color scores. However, there was no significant 
				difference among three methods of application throughout the 
				storage period though dipping had a lower rate of increase. A 
				progressive decline in mean sensory scores was recorded along 
				with the increase in storage time. 
				
				
				Conclusion: 
				
				The carrageenan and cinnamon edible coating was found to be a 
				good alternative to enhance the shelf life of chicken meat under 
				refrigeration conditions. It was also observed from study that 
				dipping method of the application had comparatively higher shelf 
				life than other methods of application. 
				
				Keywords: 
				
				carrageenan, chicken breast/fillets, cinnamon oil, edible 
				coating, spraying/brushing/dipping. 
 
              References 
 
				
					| 1. FAO. (2012) Available from: http://www.fao.org/docrep/015/i2490e/i2490e00.htm 
					Accesed on 19-11-15. |  
					|  |  
					| 2. Available from: http://www.apeda.gov.in/apedawebsite/six_head_product/animal.htm. 
					Accesed on 25-2-2015. |  
					|  |  
					| 3. Kandeepan, G., Anjaneyulu, A.S.R., Kondaiah, N. and 
					Mendiratta, S.K. (2010) Quality of buffalo meat Keema at 
					refrigerator temperature. Afr. J. Food Sci.,4(6): 410-417. |  
					|  |  
					| 4. Falguera, V., Quintero, J.P., Jimenez, A., Munoz, J.A. 
					and Ibarz, A. (2011) Edible films and coatings: Structures, 
					active functions, trends and their use. Trends Food Sci. 
					Technol., 22: 292-303. http://dx.doi.org/10.1016/j.tifs.2011.02.004
 |  
					|  |  
					| 5. Dhanapal, A., Sasikala, P., Rajamani, L., Kavitha, V., 
					Yazhini, G. and Banu, M.S. (2012) Edible films from 
					polysaccharides. Food Sci. Q. Manag., 3: 9-17. |  
					|  |  
					| 6. Sánchez-González, L., Cháfer, M., Chiralt, A. and 
					González-Martínez, C. (2010) Physical properties of edible 
					chitosan films containing bergamot essential oil and their 
					inhibitory action on Penicillium italicum. Carbohyd. Polym., 
					82: 277-283. http://dx.doi.org/10.1016/j.carbpol.2010.04.047
 |  
					|  |  
					| 7. Codex Alimentarius Commission. (2010) GSFA Online: Food 
					Additive Details, Carrageenan http://www.codexalimentarius.net/gsfaonline/additives/details.html?id=49 
					Accesed on 25-2-2015. |  
					|  |  
					| 8. Janjarasskul, T. and Krochta,J.M. (2010) Edible packaging 
					materials. Annu. Rev. Food Sci. Technol.,1: 415-148. http://dx.doi.org/10.1146/annurev.food.080708.100836
 PMid:22129343
 |  
					|  |  
					| 9. Prajapati, V.D., Maheriya, P.M., Jani, G.K. and Solanki, 
					H.K. (2014) Carrageenan: A natural seaweed polysaccharide 
					and its applications. Carbohyd. Polym., 105: 97-112. http://dx.doi.org/10.1016/j.carbpol.2014.01.067
 PMid:24708958
 |  
					|  |  
					| 10. Li, Y.Q., Kong, D.X. and Wu, H. (2013) Analysis and 
					evaluation of essential oil components of cinnamon barks 
					using GC-MS and FTIR spectroscopy. Ind. Crop Prod., 41: 
					269-278. http://dx.doi.org/10.1016/j.indcrop.2012.04.056
 |  
					|  |  
					| 11. China Pharmacopeia Commission. (2010) Pharmacopoeia of 
					the People's Republic of China. Chinese Medical Science and 
					Technology Press, Beijing, China. p163-127. |  
					|  |  
					| 12. Tzortzakis, N.G. (2009) Impact of cinnamon 
					oil-enrichment on microbial spoilage of fresh produce. 
					Innov. Food Sci. Emerg., 10: 97-102. http://dx.doi.org/10.1016/j.ifset.2008.09.002
 |  
					|  |  
					| 13. Du, H. and Li, H. (2008) Antioxidant effect of cassia 
					essential oil on deep-fried beef during the frying process. 
					Meat Sci., 78: 461-468. http://dx.doi.org/10.1016/j.meatsci.2007.07.015
 PMid:22062466
 |  
					|  |  
					| 14. Ouattara, B., Simard, R.E., Holley, R.A., Piette, G.J.P. 
					and Begin, A. (1997) Antibacterial activity of selected 
					fatty acids and essential oils against six meat spoilage 
					organisms. Int. J. Food Microbiol., 37(2-3): 155-162. http://dx.doi.org/10.1016/S0168-1605(97)00070-6
 |  
					|  |  
					| 15. Shan, B., Cai, Y., Brooks, J.D. and Corke, H. (2007) 
					Antibacterial properties and major bioactive components of 
					cinnamon stick (Cinnamomum burmannii): Activity against 
					foodborne pathogenic bacteria. J. Agric. Food Chem., 55(14): 
					5484-5490. http://dx.doi.org/10.1021/jf070424d
 PMid:17567030
 |  
					|  |  
					| 16. Sánchez-González, L., Vargas, M., Gonzalez Martinez, C., 
					Chiralt, A. and Cháfer, M. (2011) Use of essential oils in 
					bioactive edible coatings. CRC Food Eng., 3: 1-16. http://dx.doi.org/10.1007/s12393-010-9031-3
 |  
					|  |  
					| 17. USDA-FSIS. (2010) Safe and suitable ingredients used in 
					the production of meat andpoultryproducts.Directive7120.1, 
					Rev. 2. Available from: 
					http://www.fsis.usda.gov/OPPDE/rdad/FSISDirectives/7120.1Rev2.pdf. 
					Accessed on 11-01-2015. |  
					|  |  
					| 18. Trout, E.S., Hunt, M.C., Johson, D.E, Clans, J.R., 
					Castner, C.L. and Kroff, D.H. (1992) Characteristics of low 
					fat ground beef containing texture modifying ingredients. J. 
					Food Sci., 57(1): 19-24. http://dx.doi.org/10.1111/j.1365-2621.1992.tb05415.x
 |  
					|  |  
					| 19. Grau, R. and Hamm, R. (1953) Water. In: Price, J.F. and 
					Scheweigert, B.S., editors. The Science of Meat and Meat 
					Products. 2nd ed. W.H. Freemen and Company, San Francisco. 
					p186-189. |  
					|  |  
					| 20. Grau, R. and Hamm, R. (1957) In biochemistry of meat 
					hydration. Adv. Food Res., 10: 356-359. |  
					|  |  
					| 21. Pearson, D. (1968) Application of chemical methods for 
					the assessment of beef quality methods related to protein 
					breakdown. J. Sci. Food Agric., 19(7): 366-369. http://dx.doi.org/10.1002/jsfa.2740190703
 |  
					|  |  
					| 22. Somers, C., Tarrant, O.V. and Sherington, J. (1985) 
					Evaluation of some objective methods of measuring pork 
					quality. Meat Sci., 15: 63-76. http://dx.doi.org/10.1016/0309-1740(85)90047-6
 |  
					|  |  
					| 23. Strange, E.D., Benedict, R.C., Smith, J.L. and Swift, 
					C.E. (1977) Evaluation of rapid tests for monitoring the 
					alterations in meat quality during storage. J. Food Prot., 
					40: 843-847. |  
					|  |  
					| 24. Khare, A.K., Biswas, A.K. and Sahoo, J. (2014) 
					Comparison study of chitosan, EDTA, eugenol and peppermint 
					oil for antioxidant and antimicrobial potentials in chicken 
					noodles and their effect on color and oxidative stability at 
					ambient temperature storage. Food Sci. Technol., 55: 
					286-293. |  
					|  |  
					| 25. APHA. (1984) Compendium of Methods for Microbiological 
					Examination of Foods. 2nd ed. American Public Health 
					Association,Washington, DC. |  
					|  |  
					| 26. Keeton, J.T. (1983) Effect of fat and sodium chloride 
					and phosphate levels on the chemical and sensory properties 
					of pork patties. J. Food Sci., 48(3): 878-881. http://dx.doi.org/10.1111/j.1365-2621.1983.tb14921.x
 |  
					|  |  
					| 27. Snedecor, G.W. and Cochran, W.G. (1994) Statistical 
					Methods. 8th ed. The Iowa State University Press Ames, Iowa, 
					USA. |  
					|  |  
					| 28. Sinhamahapatra, M., Biswas, S., Das, A.K. and 
					Bhattacharyya, D. (2004) Comparative study of different 
					surface decontaminants on chicken quality. Br. Poultr. Sci., 
					45(5): 624-630. http://dx.doi.org/10.1080/00071660400006552
 PMid:15623215
 |  
					|  |  
					| 29. Petrou, S., Tsiraki, M., Giatrakou, V. and Savvaidis, 
					I.N. (2012) Chitosan dipping or oregano oil treatments, 
					singly or combined on modified atmosphere packaged chicken 
					breast meat. Int. J. Food Microbiol., 156: 264-271. http://dx.doi.org/10.1016/j.ijfoodmicro.2012.04.002
 PMid:22534355
 |  
					|  |  
					| 30. Kandeepan, G. and Biswas, S. (2007) Effect of low 
					temperature preservation on quality and shelf-life of 
					buffalo meat. Am. J. Food Technol., 2(3): 126-135. http://dx.doi.org/10.3923/ajft.2007.126.135
 |  
					|  |  
					| 31. James, M.J. (1966) Response of the extract-release 
					volume and water-holding capacity phenomena to 
					microbiologically spoiled beef and aged beef. Appl. 
					Microbiol., 14(4): 492-496. |  
					|  |  
					| 32. Ayadi, M.A., Kechaou, A., Makni, I. and Attia, H. (2009) 
					Influence of carrageenan additionon turkey meat sausages 
					properties. J. Food Eng., 93(3): 278-283. http://dx.doi.org/10.1016/j.jfoodeng.2009.01.033
 |  
					|  |  
					| 33. Gault, N.F.S. (1985) The relationship between water 
					holding capacity and cook meat tenderness in some beef 
					muscles as influenced by acidic conditions below the 
					ultimate pH. Meat Sci., 15(1): 15-30. http://dx.doi.org/10.1016/0309-1740(85)90071-3
 |  
					|  |  
					| 34. Rao, M. and Gault, N.F.S. (1989) The influence of 
					fibre-type composition and associated biochemical 
					characteristics on the acid buffering capacities of several 
					beef muscles. Meat Sci., 26: 5-18. http://dx.doi.org/10.1016/0309-1740(89)90053-3
 |  
					|  |  
					| 35. Gheisari, H.R. (2011) Correlation between acid, TBA, 
					peroxide and iodine values, catalase and glutathione 
					peroxidase activities of chicken, cattle and camel meat 
					during refrigerated storage. Vet. World, 4(4): 153-157. http://dx.doi.org/10.5455/vetworld.2011.153-157
 |  
					|  |  
					| 36. Wu, Y., Rhim, J.W., Weller, C.L., Hamouz, F., Cuppett, 
					S. and Schnepf, M. (2000) Moisture loss and lipid oxidation 
					for precooked beef patties store in edible coatings and 
					films. J. Food Sci., 65: 300-304. http://dx.doi.org/10.1111/j.1365-2621.2000.tb15997.x
 |  
					|  |  
					| 37. Pikul, J., Leszcynski, D.E., Niewiarowicz, A. and 
					Kummerow, F.A. (1984) Lipid oxidation in chicken breast and 
					leg meat after sequential treatments of frozen storage, 
					cooking, refrigerated storage and reheating. J. Food 
					Technol., 19: 575-580. http://dx.doi.org/10.1111/j.1365-2621.1984.tb01874.x
 |  
					|  |  
					| 38. Sheikh Dalia, M.E. (2014) Efficiency of using Arabic gum 
					and plantago seeds mucilage as edible coating for chicken 
					boneless breast. Food Sci. Q. Manag., 32: 28-33. |  
					|  |  
					| 39. Qiu, X., Chen, S., Liu, G. and Yang, Q. (2014) Quality 
					enhancement in the Japanese sea bass (Lateolabrax japonicas) 
					fillets stored at 4°C by chitosan coating incorporated with 
					citric acid or licorice extract. Food Chem., 162: 156-160. http://dx.doi.org/10.1016/j.foodchem.2014.04.037
 PMid:24874371
 |  
					|  |  
					| 40. Kamel, S.M. (2014) Utilization of cactus dear peels 
					mucilage as an edible coating of chicken meat to prolong its 
					shelf life. Food Sci. Q. Manag., 28: 71-77. |  
					|  |  
					| 41. Dainty, R.H., Shaw, B.G., Boer, K.A. and Scheps, E.S.J. 
					(1975) Protein changes caused by bacterial growth on beef. 
					J. Appl. Bacteriol., 39(1): 73-81. http://dx.doi.org/10.1111/j.1365-2672.1975.tb00547.x
 |  
					|  |  
					| 42. Biswas, S., Chakraborty, A., Patra, G. and Dhargupta, A. 
					(2011) Quality and acceptability of duck patties stored at 
					ambient and refrigeration temperature. Int. J. Livest. 
					Prod., 1(1): 1-6. |  
					|  |  
					| 43. Kester, J.J. and Fennema, O. (1986) Edible films and 
					coatings: A review. Food Technol., 40: 47-59. |  
					|  |  
					| 44. Pearce, J.A. and Lavers, C.G. (1949). Frozen storage of 
					poultry. V. Effects of some processing factors on quality. 
					Can. J. Res., 27: 253-265. http://dx.doi.org/10.1139/cjr49f-026
 |  
					|  |  
					| 45. Lesiak, M.T., Olson, D.G., Lesiak, C.A. and Ahn, D.U. 
					(1996) Effects of postmortem temperature and time in 
					water-holding capacity of hot-boned turkey breast and thigh 
					muscle. Meat Sci., 43(1): 51-60. http://dx.doi.org/10.1016/0309-1740(95)00062-3
 |  
					|  |  
					| 46. Lee, Y.S., Owens, C.M. and Meullenet, J.F. (2009) 
					Changes in tenderness, color, and water holding capacity of 
					broiler breast meat during post deboning aging. J. Food 
					Sci., 74(8): 449-454. http://dx.doi.org/10.1111/j.1750-3841.2009.01332.x
 PMid:19799666
 |  
					|  |  
					| 47. Komoltri, P. and Pakdeechanuan, P. (2012) Effects of 
					marinating ingredients on physicochemical, microstructural 
					and sensory properties of Golek chicken. Int. Food Res. J., 
					19(4): 1449-1455. |  
					|  |  
					| 48. Ke, S., Huang, Y., Decker, E.A. and Hultin, H.O. (2009) 
					Impact of citric acid on the tenderness, microstructure and 
					oxidative stability of beef muscle. Meat Sci., 82: 113-118. http://dx.doi.org/10.1016/j.meatsci.2008.12.010
 PMid:20416613
 |  
					|  |  
					| 49. Machado de Melo,A.A., Geraldine, R.M., Araujo Silveira, 
					M.F., Torres, M.C.L., Rezende, C.S.M., Fernandes, T.H. and 
					Oliveira, A.N. (2012) Microbiological quality and other 
					characteristics of refrigerated chicken meat in contact with 
					cellulose acetate-based film incorporated with rosemary 
					essential oil. Braz. J. Microbiol., 43(4): 1419-1427. http://dx.doi.org/10.1590/S1517-83822012000400025
 |  
					|  |  
					| 50. Tyburcy, A. and Kozyra, D. (2010) Effects of composite 
					surface coating and pre-drying on the properties of Kabanosy 
					dry sausage. Meat Sci., 86(2): 405-410. http://dx.doi.org/10.1016/j.meatsci.2010.05.025
 PMid:20566245
 |  
					|  |  
					| 51. Chouliara, E., Badeka, A., Savvaidis, I. and Kontominas, 
					M.G. (2008) Combined effect of irradiation and modified 
					atmosphere packaging on shelf life extension of chicken 
					breast meat: Microbiological, chemical and sensory changes. 
					Eur. Food Res. Technol., 226(4): 877-888. http://dx.doi.org/10.1007/s00217-007-0610-3
 |  
					|  |  
					| 52. Keokamnerd, T., Acton, J.C., Han, I.Y. and Dawson, P.L. 
					(2008) Effect of commercial rosemary oleoresin preparations 
					on ground chicken thigh meat quality packaged in a 
					high-oxygen atmosphere. Poultr. Sci., 87: 170-179. http://dx.doi.org/10.3382/ps.2007-00066
 PMid:18079467
 |  
					|  |  
					| 53. Rodríguez-Calleja, J.M., Cruz-Romero, M.C., O'Sallivan, 
					M.G., García-López, M.L. and Kelly, J.P. (2012) 
					High-pressure-based hurdle strategy to extend the shelf-life 
					of fresh chicken fillets. Food Control., 25: 516-524. http://dx.doi.org/10.1016/j.foodcont.2011.11.014
 |  
					|  |  
					| 54. Lu, F., Liu, D.H., Ye, X.Q., Wei, Y.X. and Liu, F. 
					(2010) Alginate calcium coating incorporating nisin and EDTA 
					maintains the quality of fresh northern snakehead (Channa 
					argus) fillets stored at 4°C. J. Sci. Food Agric., 89: 
					848-854. http://dx.doi.org/10.1002/jsfa.3523
 |  
					|  |  
					| 55. Giatrakou, V., Ntzimani, A., Zwietering, M. and 
					Savvaidis, I.N. (2010) Combined chitosan thyme treatments 
					with modified atmosphere packaging on a Greek ready-to-cook 
					(RTC) poultry product. J. Food Prot., 73: 663-669. PMid:20377954
 |  
					|  |  
					| 56. ICMSF (International Commission on Microbiological 
					Specifications for Foods). (1986) Microorganisms in Foods. 
					2. Sampling for Microbiological Analysis: Principles and 
					Scientific Applications. 2nd ed. University of Toronto 
					Press, Toronto. |  
					|  |  
					| 57. Shan, B.E., Yoshida, Y., Sugiura, T. and Yamashita, U. 
					(1999) Stimulating activity of Chinese medicinal herbs on 
					human lymphocytes in vitro. Int. J. Immunopharmacol., 21: 
					149-159. http://dx.doi.org/10.1016/S0192-0561(98)00074-5
 |  
					|  |  
					| 58. Ojagh,S.M., Rezaei, M., Razavi, S.H. and Hosseini, 
					S.M.H. (2010) Effect of chitosan coatings enriched with 
					cinnamon oil on the quality of refrigerated rainbow trout. 
					Food Chem., 120: 1193-1198. http://dx.doi.org/10.1016/j.foodchem.2009.10.006
 |  
					|  |  
					| 59. Seol, K.H., Lim, D.G., Jang, A., Jo, C. and Lee, M. 
					(2009) Antimicrobial effect of κ-carrageenan-based edible 
					film containing ovotransferrin in fresh chicken breast 
					stored at 5°C. Meat Sci., 83(3): 479-483. http://dx.doi.org/10.1016/j.meatsci.2009.06.029
 PMid:20416673
 |  
					|  |  
					| 60. Shojaee-Aliabadi, S., Hosseini, H., Mohammadifar, M.A., 
					Mohammadi, A., Ghasemlou, M. and Ojagh, S.M. (2013) 
					Characterization of antioxidant antimicrobial j-carrageenan 
					films containing Satureja hortensis essential oil. Int. J. 
					Biol. Macromol., 52(1): 116-124. http://dx.doi.org/10.1016/j.ijbiomac.2012.08.026
 PMid:22959956
 |  
					|  |  
					| 61. Olaimat, A.N., Fang, Y. and Holley, R.A. (2014) 
					Inhibition of campylobacter jejuni on fresh chicken breasts 
					by κ-carrageenan/chitosan-based coatings containing allyl 
					isothiocyanate or deodorized oriental mustard extract. Int. 
					J. Food Microbiol., 187:77-82. http://dx.doi.org/10.1016/j.ijfoodmicro.2014.07.003
 PMid:25058687
 |  
					|  |  
					| 62. Cierach, M., Modzelewska-Kapituła,M. and Szaciło, K. 
					(2009) The influence of carrageenan on the properties of 
					low-fat frankfurters. Meat Sci., 82(3): 295-299. http://dx.doi.org/10.1016/j.meatsci.2009.01.025
 PMid:20416731
 |  
					|  |  
					| 63. Baston, O. and Barna, O. (2010) Raw Chicken Leg and 
					breast sensory evaluation. Ann. Food Sci. Technol., 11(1): 
					50-53. |  
					|  |  
					| 64. Mexis, S.F., Chouliara, E. and Kontominas, M.G. (2012) 
					Shelf life extension of ground meat using an oxygen absorber 
					and a citrus extract. Food Sci. Technol., 49: 21-27. http://dx.doi.org/10.1016/j.lwt.2012.04.012
 |  
					|  |  
					| 65. Del Rio, E., Muriente, R., Prieto, M., Alosnso-Calleja, 
					C. and Capite. R. (2007) Effectiveness of trisodium 
					phosphate, acidified sodium chlorite, citric acid and 
					peroxyacids against pathogenic bacteria on poultry during 
					refrigerated storage. J. Food Prot.,70(9): 2063-2071. PMid:17900083
 |  |