Open Access
Research
(Published
online:
16-02-2016)
11.
Isolation, antibiogram and pathogenicity of
Salmonella
spp. recovered from slaughtered food
animals in Nagpur region of Central India -
D. G. Kalambhe, N. N. Zade, S. P. Chaudhari, S. V. Shinde, W.
Khan and A. R. Patil
Veterinary World, 9(2): 176-181
doi:
10.14202/vetworld.2016.176-181
D. G. Kalambhe:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; drdeepalikalambhe@gmail.com
N. N. Zade:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; n_zade@rediffmail.com
S. P. Chaudhari:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; sandeep_vph@gmail.com
S. V. Shinde:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; shilpi_shri5@rediffmail.com
W. Khan:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; waqar_khan73@rediffmail.com
A. R. Patil:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; drarchanavph@gmail.com
Received: 11-08-2015, Revised: 24-12-2015, Accepted: 31-12-2015,
Published online: 16-02-2016
Corresponding author:
D. G. Kalambhe, e-mail: drdeepalikalambhe@gmail.com
Citation:
Kalambhe DG, Zade NN, Chaudhari SP, Shinde SV, Khan W, Patil AR
(2016) Isolation, antibiogram and pathogenicity of
Salmonella
spp. recovered from slaughtered food animals in Nagpur region of
Central India,
Veterinary World 9(2):
176-181.
Abstract
Aim:
To determine the prevalence, antibiogram and pathogenicity of
Salmonella
spp. in the common food animals slaughtered for consumption
purpose at government approved slaughter houses located in and
around Nagpur region during a period of 2010-2012.
Materials and Methods:
A total of 400 samples comprising 50 each of blood and meat from
each slaughtered male cattle, buffaloes, pigs and goats were
collected. Isolation was done by pre-enrichment in buffered
peptone water and enrichment in Rappaport-Vassiliadis broth with
subsequent selective plating onto xylose lysine deoxycholate
agar. Presumptive
Salmonella
colonies were biochemically confirmed and analyzed for
pathogenicity by hemolysin production and Congo red dye binding
assay (CRDA). An antibiotic sensitivity test was performed to
assess the antibiotic resistance pattern of the isolates.
Results:
A total of 10 isolates of
Salmonella
spp. from meat (3 from cattle, 1 from buffaloes and 6 from pigs)
with an overall prevalence of 5% among food animals was
recorded. No isolation was reported from any blood samples.
Pathogenicity assays revealed 100% and 80% positivity for CRDA
and hemolytic activity, respectively. Antimicrobial sensitivity
test showed multi-drug resistance. The overall resistance of 50%
was noted for trimethoprim followed by ampicillin (20%). A
maximum sensitivity (80%) was reported to gentamycin followed by
40% each to ampicillin and trimethoprim, 30% to amikacin and 10%
to kanamycin.
Conclusion:
The presence of multidrug resistant and potentially pathogenic
Salmonella
spp. in slaughtered food animals in Nagpur region can be a
matter of concern for public health.
Keywords:
antibiogram, Congo red binding assay, food animals, hemolysis,
Salmonella
Typhimurium.
References
|
1. Pegues, D.A., Oh, M.E. and Miller, S.I. (2005) Salmonella
species, including Salmonella Typhi. In: Mandel, G.L.,
Bennett, J.E., Dolin, R., editors. Mandell, Douglas and
Bennett's Principles and Practice of Infectious Diseases.
6th ed. Elsevier Churchill Livingstone, New York.
p2636-2654. |
|
|
2. Baker, M.G., Thorns, C., Lopez, L.D., Garrett, N.K. and
Nikol, C.M. (2007) A recurring Salmonellosis epidemic in New
Zealand linked to contact with sheep. Epidemiol. Infect.,
135: 76-83.
http://dx.doi.org/10.1017/S0950268806006534
PMid:16740191 PMCid:PMC2870545 |
|
|
|
3. World Health Organization. (2006) 6th International
Conference on Typhoid Fever and other Salmonellosis, Geneva. |
|
|
|
4. Nyeleti, C., Molla, B., Hilderbandt, G. and Kleer, J.
(2000) The prevalence and distribution of Salmonella in
slaughter cattle, slaughterhouse personnel and minced beef
in Addis Ababa, Ethiopia. Bull. Anim. Health Prod. Afr., 48:
19-24. |
|
|
|
5. Molla, B., Alemayehmu, D. and Salah, W. (1997-2002)
Sources and distribution of Salmonella serotypes isolated
from food animals, slaughterhouse personnel and retail meat
products in Ethiopia, 2003. Ethiop. J. Health Dev., 17:
63-70. |
|
|
|
6. D'Aoust, J.Y. (1989) Salmonella. In: Doyle, M.P., editor.
Foodborne Bacterial Pathogens. Marcel Dekker Inc., New York. |
|
|
7. Nair, A., Balasaravanan, T., Malik, S.V.S., Mohan, V.,
Kumar, M., Vergis, J. and Deepak, B.R. (2015) Isolation and
identification of Salmonella from diarrheagenic infants and
young animals, sewage waste and fresh vegetables. Vet.
World, 8(5): 669-673.
http://dx.doi.org/10.14202/vetworld.2015.669-673 |
|
|
8. Maharjan, M., Joshi, V., Joshi, D.D. and Manandhar, P.
(2006) Trends in the study of disease agents. Ann N Y Acad.
Sci., 1081: 249-256.
http://dx.doi.org/10.1196/annals.1373.031
PMid:17135520 |
|
|
|
9. Kshirsagar, D.P., Singh, S., Brahmbhatt, M.N. and Nayak,
J.B. (2014) Isolation and molecular characterization of
virulence-associated genes of Salmonella from buffalo meat
samples in Western region of India. Israel J. Vet. Med.,
69(4): 228-233. |
|
|
10. Boonmar, S., Markvichitr, K., Chaunchom, S., Chanda, C.
and Bangtrakulnonth, A. (2008) Salmonella prevalence in
slaughtered buffaloes and pigs and antimicrobial
susceptibility of isolates in Vientiane, Lao People's
Democratic Republic. J. Vet. Med. Sci., 70: 1345-1348.
http://dx.doi.org/10.1292/jvms.70.1345
PMid:19122403 |
|
|
|
11. Khan, J.A., Rathore, R.S., Khan, S. and Ahmad, I.
(2013). Prevalence, characterization and detection of
Salmonella spp. from various meat sources. Adv. Anim. Vet.
Sci., 1(1S): 4-8. |
|
|
|
12. Arslan, S. and Ayla, E. (2010) Occurrence and
antimicrobial resistance profile of Salmonella species in
retail meat products. J. Food Prot., 73(9): 10-63. |
|
|
|
13. Rathod, K.S., Ambadkar, R.K., Zanjad, P.N., Deshmukh,
V.V. and Raziuddin, M. (2004) Microbiological quality of
chevon sold in Parbhani City. J. V.P.H., 2: 19-22. |
|
|
|
14. Makwana, P.P., Nayak, J.B., Brahmbhatt, M.N. and
Chaudhary, J.H. (2015) Detection of Salmonella spp. from
chevon, mutton and its environment in retail meat shops in
Anand city (Gujarat), India, Vet. World, 8(3): 388-392. |
|
|
15. Shashidhar, R., Srivastava, I. and Bandekar, J.R. (2011)
Quantification of Salmonella in food samples from India
using the MINI-MSRV MPN and modified MINI-MSRV MPN methods.
J. Food Sci., 76(8): M564-M567.
http://dx.doi.org/10.1111/j.1750-3841.2011.02366.x |
|
|
|
16. Arvind, K., Khajuria, P. and Kaur, M. (2011) Tracking of
pathogenic and spoilage microbes in mutton and chevon
processing in retail markets of Jammu. Indian J. Small
Rumin., 17(1): 74-77. |
|
|
|
17. Wassie, M.A. (2004) A cross-sectional study on
Salmonella in apparently healthy slaughtered sheep and goats
at Addis Ababa and Modjo abattoirs, Ethiopia. Master thesis,
Addis Ababa University, Faculty of Veterinary Medicine,
Debre Zeit, Ethiopia. |
|
|
18. Chandra, M., Singh, B.R., Shankar, H., Agarwal, M.,
Agarwal, R.K., Sharma, G. and Babu, N. (2007) Prevalence of
Salmonella antibodies among goats slaughtered for chevon in
Bareilly (northern India). Prev. Vet. Med., 80: 1-8.
http://dx.doi.org/10.1016/j.prevetmed.2007.01.008
PMid:17336413 |
|
|
|
19. Moon, A.H. (2011) Studies on: Prevalence and antibiogram
of Salmonella species of polluted meat origin. Asiatic J.
Biotechnol. Resour., 2(04): 447-453. |
|
|
20. Kumar, T., Rajora, V.S. and Arora, N. (2013) Prevalence
of Salmonella in pigs and broilers in the Tarai region of
Uttarakhand, India. Indian J. Med. Microbiol., 32(1):
99-101.
http://dx.doi.org/10.4103/0255-0857.124356
PMid:24399408 |
|
|
|
21. Yemisi, A., Alli Oyebode, T., Margaret, A. and Justina,
J. (2011) Prevalence of Arcobacter, Escherichia coli,
Staphylococcus aureus and Salmonella species in retail raw
chicken, pork, beef and goat meat in Osogbo, Nigeria. Sierra
Leone J. Biomed. Res., 3(1): 8-12. |
|
|
|
22. Ejeta, G., Molla, B., Alemayehu, D. and Muckle, A.
(2004) Salmonella serotypes isolated from minced meat beef,
mutton and pork in Addis Ababa, Ethiopia. Rev. Med. Vet.,
155(11): 547-551. |
|
|
23. Letellier, A., Messier, S. and Quessy, S. (1999)
Prevalence of Salmonella spp. and Yersinia enterocolitica in
finishing Swine at Canadian abattoirs. J. Food Prot., 62(1):
22-25.
PMid:9921823 |
|
|
24. Andreshel, J.K., Sorensen, R. and Glensbjerg, M. (1991)
Aspects of the epidemiology of Yersinia enterocolitica: A
review. Int. J. Food Microbiol., 13(3): 231-237.
http://dx.doi.org/10.1016/0168-1605(91)90007-C |
|
|
|
25. Kumara, P., Rao, J., Haribabuc, Y. and Manjunath, D.
(2014) Microbiological quality of meat collected from
municipal slaughter houses and retail meat shops from
Hyderabad Karnataka region, India. APCBEE Proc., 8: 364-369. |
|
|
|
26. El-Taib, K.A. (2011) Studies on virulence characters of
Salmonella Typhimurium isolated from animal and human. Rep.
Opin., 3(7): 37-43. |
|
|
|
27. Agrawal, M., Chandra, M., Sharma, G. and Singh, B.R.
(2005) A study on virulence markers of Indian strain of
Salmonella Enterica subspecies enterica serovar Paratyphi B
from foods of animal origin. J. Food Sci. Technol., 42:
66-79. |
|
|
28. Tiwari, R.P., Deol, K., Rishi, P. and Grewal, J.S.
(2002) Factors affecting haemolysin production and Congo red
binding in Salmonella Enterica serovar Typhimurium DT 98. J.
Med. Microbiol., 51: 50.
http://dx.doi.org/10.1099/0022-1317-51-6-503
PMid:12018658 |
|
|
|
29. Zade, N.N. and Karpe, A.G. (2010) Final scheme report on
characterization of plasmid DNA of the bacteria from foods
of animal origin. A Report Submitted to Indian Council of
Agriculture Research, New Delhi. p11. |
|
|
30. Singh, B.R., Agarwal, M., Chandra, M., Verma, M.,
Sharma, G., Verma, J.C. and Singh, V.P. (2010) Plasmid
profile and drug resistance pattern of zoonotic Salmonella
isolates from Indian buffaloes. J. Infect. Dev. Ctries.,
4(8): 477-483.
http://dx.doi.org/10.3855/jidc.713 |
|
