Open Access
Research
(Published
online:
31-01-2016)
17.
Anthelmintic activity of
Indigofera tinctoria
against gastrointestinal nematodes of
sheep -
Ambalathaduvar Meenakshisundaram, Tirunelveli Jayagopal
Harikrishnan and Thavasi Anna
Veterinary World, 9(1): 101-106
doi:
10.14202/vetworld.2016.101-106
Ambalathaduvar Meenakshisundaram:
Department of Veterinary Parasitology, Veterinary College and
Research Institute,
Tirunelveli - 627 358, Tamil Nadu, India; fish1092@rediffmail.com
Tirunelveli Jayagopal Harikrishnan:
Registrar, Tamil Nadu Veterinary and Animal Sciences University,
Chennai - 600051, Tamil Nadu, India; tjkrish@gmail.com
Thavasi Anna:
Department of Veterinary Parasitology, Veterinary College and
Research Institute, Tirunelveli - 627 358, Tamil Nadu, India;
drtanna@rediffmail.com
Received: 15-08-2015, Revised: 08-12-2015, Accepted: 18-12-2015,
Published online: 31-01-2016
Corresponding author:
Ambalathaduvar
Meenakshisundaram, e-mail: fish1092@rediffmail.com
Citation:
Meenakshisundaram A, Harikrishnan TJ, Anna T (2016),
Anthelmintic activity of
Indigofera tinctoria
against gastrointestinal nematodes of sheep,
Veterinary World
9(1): 101-106.
Abstract
Aim:
Gastrointestinal (GI) nematodes are considered as a major
constraint for successful sheep production. Control of these
parasites heavily relies on the use of chemical anthelmintics.
Over the past decades, the development of anthelmintic
resistance to various groups of anthelmintics and problem of
drug residues in animal products has awakened interest in
medicinal plants as an alternative source of anthelmintics.
Hence, this study was undertaken to evaluate the anthelmintic
efficacy of
Indigofera tinctoria
by scientifically validated
in vitro
and
in vivo
tests approved by the World Association for the Advancement of
Veterinary Parasitology.
Materials and Methods:
In vitro
assays such as egg hatch assay for ovicidal and larval migration
inhibition and larval development assay for larvicidal
properties were used to investigate
in vitro
effect of extracts on strongyle egg and larvae, respectively.
Fecal egg count reduction test was conducted
in vivo
to evaluate the therapeutic efficacy of the extracts
administered orally at dose rates of 125, 250, 500 mg/kg to
sheep naturally infected with mixed GI nematodes.
Results:
Ethanolic extract of
I. tinctoria
demonstrated significant (p<0.01) inhibition on egg hatching at
concentrations of 40 mg/ml and 80 mg/ml. In
in vivo
assay, the ethanolic extract of
I. tinctoria
reduced the fecal egg count ranging between 30.82% and 47.78% at
various doses (125, 250 and 500 mg/kg). Although there was a
slight variation, all the hematological parameters were within
the normal range reported for sheep. Except for alanine
transaminase, the overall mean of all the serum biochemical
profile was within the normal range for sheep.
Conclusion:
Based on the results obtained by
in vitro
and
in vivo
assay, the ethanolic extract of
I. tinctoria
possesses anthelmintic activity and could replace the chemical
anthelmintics used presently.
Keywords:
anthelmintic, evaluation, gastrointestinalnematodes,
Indigofera tinctoria,
sheep.
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