Open Access
Research
(Published
online:
31-01-2016)
18.
Comparison of rapid immunodiagnosis assay
kit with molecular and immunopathological approaches for
diagnosis of rabies in cattle -
Ajaz Ahmad and C. K. Singh
Veterinary World, 9(1): 107-112
doi:
10.14202/vetworld.2016.107-112
Ajaz Ahmad:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana - 141 004, Punjab, India; ajazpatho786@gmail.com
C. K. Singh:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana - 141 004, Punjab, India; rabiesck@gmail.com
Received: 09-10-2015, Revised: 20-12-2015, Accepted: 28-12-2015,
Published online: 31-01-2016
Corresponding author:
Ajaz Ahmad, e-mail: ajazpatho786@gmail.com
Citation:
Ahmad A, Singh CK (2016), Comparison of rapid immunodiagnosis
assay kit with molecular and immunopathological approaches for
diagnosis of rabies in cattle,
Veterinary World 9(1):
107-112.
Abstract
Aim:
Presently, diagnosis of rabies is primarily based on,
conventional fluorescent antibody technique (FAT),
immunopathological and molecular techniques. Recently, rapid
immunodiagnostic assay (RIDA) - A monoclonal antibodybased
technique has been introduced for rapid diagnosis of rabies. The
present investigation is envisaged to study the efficacy of RIDA
kit for the diagnosis of rabies in cattle.
Materials and Methods:
About 11 brain samples from cattle, clinically suspected for
rabies, were screened by the FAT, Heminested reverse
transcriptase polymerase chain reaction (HnRT-PCR),
Immunohistochemistry (IHC), and RIDA.
Results:
The sensitivity for detection of rabies from brain tissue by
RIDA was 85.7% as compared to 100% by IHC as well as HnRT-PCR.
The accuracy of detection of rabies by RIDA was 91.6% as
compared to 100% that of IHC and HnRT-PCR, whereas specificity
of RIDA was 100% like that of the IHC and HnRT-PCR.
Conclusion:
Despite a comparatively low-sensitivity and accuracy of RIDA,
latter can still be useful in screening a large number of field
samples promptly. However, it is recommended that negative
results with RIDA in cattle need to be authenticated by suitable
alternative diagnostic approaches.
Keywords:
cattle, diagnosis, fluorescent antibody technique, heminested
reverse transcriptase, immunohistochemistry, rabies.
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