Open Access
Research
(Published
online: 17-07-2016)
11.
Seroprevalence of human brucellosis in and
around Jammu, India, using different serological tests -
H. K. Sharma, S. K. Kotwal, D. K. Singh, M. A. Malik, Arvind
Kumar, Rajagunalan
and M. Singh
Veterinary World, 9(7): 742-746
doi:
10.14202/vetworld.2016.742-746
H. K. Sharma :
Division of
Veterinary Public Health and Epidemiology, Faculty of Veterinary
Sciences and Animal Husbandry, Sher-e-Kashmir University of
Agricultural Sciences and Technology, Jammu, Jammu and Kashmir,
India; harshvphe@gmail.com
S. K. Kotwal :
Division of
Veterinary Public Health and Epidemiology, Faculty of Veterinary
Sciences and Animal Husbandry, Sher-e-Kashmir University of
Agricultural Sciences and Technology, Jammu, Jammu and Kashmir,
India; kotwalsk@yahoo.co.in
D. K. Singh :
Division of
Veterinary Public Health, Brucella Laboratory, Indian Veterinary
Research Institute, Izzatnagar, Bareilly, Uttar Pradesh, India;
dks_bly@yahoo.com
M. A. Malik :
Division of
Veterinary Public Health and Epidemiology, Faculty of Veterinary
Sciences and Animal Husbandry, Sher-e-Kashmir University of
Agricultural Sciences and Technology, Jammu, Jammu and Kashmir,
India; malikskuast@yahoo.com
Arvind Kumar :
Division of
Livestock Products Technology, Faculty of Veterinary Sciences
and Animal Husbandry, Sher-e-Kashmir University of Agricultural
Sciences and Technology, Jammu, Jammu and Kashmir, India;
drarvindlpt@gmail.com
Rajagunalan :
Division of
Veterinary Public Health, Brucella Laboratory, Indian Veterinary
Research Institute, Izzatnagar, Bareilly, Uttar Pradesh, India;
drgunavet@gmail.com
M. Singh :
Division of
Veterinary Public Health and Epidemiology, Faculty of Veterinary
Sciences and Animal Husbandry, Sher-e-Kashmir University of
Agricultural Sciences and Technology, Jammu, Jammu and Kashmir,
India; manindersingh2k2@gmail.com
Received: 24-04-2016, Accepted: 11-06-2016, Published online:
17-07-2016
Corresponding author:
H. K.
Sharma, e-mail: harshvphe@gmail.com
Citation:
Sharma HK, Kotwal SK, Singh DK, Malik MA, Kumar A, Rajagunalan,
Singh M (2016) Seroprevalence of human brucellosis in and around
Jammu, India, using different serological tests, Veterinary
World, 9(7): 742-746.
Abstract
Aim:
Brucellosis
is a disease of zoonotic importance as it affects both human as
well as animal’s health, and therefore, directly affects animal
productivity and human efficiency. Therefore, a study was
conducted to estimate the seroprevalence of brucellosis in
humans in Jammu and surrounding areas.
Materials and
Methods:
A total of 121
sera samples from humans occupied with professional related to
animals were collected and tested for anti-Brucella
antibodies by Rose Bengal plate test (RBPT), modified RBPT (mRBPT),
standard tube agglutination test (STAT), and indirect
enzyme-linked immunosorbent assay (I-ELISA). Sampling was done
keeping in view with the occupation, sex, and age.
Results:
The
overall seroprevalence of brucellosis recorded was 4.96%. The
test-wise seroprevalence was 9.91% by RBPT, 9.91% by mRBPT,
9.09% by STAT, and 16.52% by I-ELISA. The prevalence of
brucellosis was higher in >35-50 years age group compared to
>20-35 years and >50-65 years. Sex-wise seroprevalence was
higher in males than females. Taking I-ELISA as standard, the
relative sensitivities of mRBPT, RBPT, and I-ELISA were in the
order of mRBPT=RBPT>STAT. All the tests revealed high
specificity values; however, among different serological tests,
I-ELISA detected a maximum number of positive sera samples.
Conclusions:
The
prevalence of brucellosis was found to be approximately 5%. The
adult (>35-50 years) age male group was most vulnerable. The
routine diagnosis of brucellosis involved the conventional
serological tests, viz., RBPT and STAT, but each was
associated with drawbacks which could give either false-positive
or false-negative interpretation. Therefore, it is always
recommended to use a battery of tests in the diagnosis of
brucellosis.
Keywords:
brucellosis, humans, indirect enzyme-linked immunosorbent assay,
Rose Bengal plate test, seroprevalence, standard tube
agglutination test.
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