Open Access
Research
(Published
online: 26-07-2016)
15.
Effect of oral administration of Bacillus coagulans B37
and Bacillus pumilus B9 strains on fecal coliforms,
Lactobacillus and Bacillus spp. in rat animal model -
Lopamudra Haldar and D. N. Gandhi
Veterinary World, 9(7): 766-772
doi:
10.14202/vetworld.2016.766-772
Lopamudra Haldar:
Department of Basic Sciences, Faculty of Science and Technology,
ICFAI University, Kamalghat - 799 210, Tripura, India; mohor7@gmail.com
D. N. Gandhi:
Dairy Microbiology Division, National Dairy Research Institute,
Karnal - 132 001, Haryana, India; DNG: dngandhi@rediffmail.com
Received: 18-03-2016, Accepted: 16-06-2016, Published:
26-07-2016
Corresponding author:
Lopamudra Haldar, e-mail: mohor7@gmail.com
Citation:
Haldar L, Gandhi DN (2016) Effect of oral administration of
Bacillus coagulans B37 and Bacillus pumilus B9
strains on fecal coliforms, Lactobacillus and Bacillus
spp. in rat animal model, Veterinary World, 9(7):
766-772.
Abstract
Aim:
To investigate the effect of oral administration of two
Bacillus strains on fecal coliforms, Lactobacillus
and Bacillus spp. in rat animal model.
Materials and Methods:
An in vivo experiment was conducted for 49-day period on
36 adult male albino Wister rats divided equally into to four
groups. After 7-day adaptation period, one group (T1) was fed on
sterile skim milk along with basal diet for the next 28 days.
Second (T2) and (T3) groups received spore biomass of
Bacillus coagulans B37 and Bacillus pumilus B9,
respectively, suspended in sterilized skim milk at 8-9 log
colony-forming units/ml plus basal diet for 28 days, while
control group (T4) was supplied with clean water along with
basal diet. There was a 14-day post-treatment period. A total of
288 fecal samples (8 fecal collections per rat) were collected
at every 7-day interval starting from 0 to 49 days and subjected
to the enumeration of the counts of coliforms and lactobacilli
and Bacillus spores using respective agar media. In
vitro acid and bile tolerance tests on both the strains were
performed.
Results:
The rats those (T2 and T3) received either B. coagulans
B37 or B. pumilus B9 spore along with non-fermented skim
milk showed decrease (p<0.01) in fecal coliform counts and
increase (p<0.05) in both fecal lactobacilli and Bacillus
spore counts as compared to the control group (T4) and the group
fed only skim milk (T1). In vitro study indicated that
both the strains were found to survive at pH 2.0 and 3.0 even up
to 3 h and tolerate bile up to 2.0% concentration even after 12
h of exposure.
Conclusions:
This study revealed that oral administration of either B.
coagulans B37 or B. pumilus B9 strains might be
useful in reducing coliform counts accompanied by concurrent
increase in lactobacilli counts in the intestinal flora in rats.
Keywords:
acid salt tolerance, antibacterial activity, Bacillus
coagulans, Bacillus pumilus, bile salt tolerance,
probiotics.
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