Open Access
Research
(Published
online: 17-06-2016)
11.
Effect of egg yolk powder on freezability of Murrah buffalo (Bubalus
bubalis)
semen -
N. Kumar, S. A. Lone, J. K. Prasad, M. H. Jan and S. K. Ghosh
Veterinary World, 9(6): 601-604
doi:
10.14202/vetworld.2016.601-604
N. Kumar:
Division of Animal Reproduction, Gynecology & Obstetrics, NDRI,
Karnal, Haryana, India; drnishantvet@yahoo.com
S. A. Lone:
Division of Animal Reproduction, Gynecology & Obstetrics, NDRI,
Karnal, Haryana, India; drloneshabir@gmail.com
J. K. Prasad:
Germ Plasm Centre, Division of Animal Reproduction, Indian
Veterinary Research Institute, Izatnagar, Bareilly, Uttar
Pradesh, India; jkprasad2001@yahoo.com
M. H. Jan:
Central Institute for Research on Buffalo, Hisar, Haryana,
India; mhjanivri@gmail.com
S. K. Ghosh:
Germ Plasm Centre, Division of Animal Reproduction, Indian
Veterinary Research Institute, Izatnagar, Bareilly, Uttar
Pradesh, India; skghoshivri@yahoo.com
Received: 05-02-2016, Accepted: 03-05-2016, Published online:
17-06-2016
Corresponding author:
N. Kumar, e-mail: drnishantvet@yahoo.com
Citation:
Kumar N, Lone SA, Prasad JK, Jan MH, Ghosh SK (2016) Effect of
egg yolk powder on freezability of Murrah buffalo (Bubalus
bubalis)
semen,
Veterinary World, 9(6):
601-604.
Abstract
Aim:
The aim of this study was to investigate the effect of
commercial egg yolk powder as an alternative to fresh egg yolk
on freezability of Murrah buffalo semen.
Materials and Methods:
Semen samples (12) from 3 Murrah buffaloes (4 from each bull)
with mass motility (≥3+) and total motility (70% and above) were
utilized in this study. Immediately after collection, each
sample was divided into four groups. Groups I was diluted up to
60×106 sperm/ml with tris extender containing 10%
fresh egg yolk and Groups II, III, and IV were diluted up to
60×106 sperm/ml with tris extender containing 2%, 4%,
and 6% egg yolk powder, respectively. Semen samples were
processed and cryopreserved followed by examination of frozen
semen samples after 24 h. Semen samples from each group were
evaluated for total motility, viability, acrosomal integrity,
abnormality, and hypo-osmotic swelling test (HOST) response
after dilution, pre-freeze, and post-thaw stage.
Results:
Pre-freeze total motility was significantly (p<0.05) higher in
Groups III and IV as compared to Groups I and II, and post-thaw
total motility was significantly (p<0.01) higher in Group III as
compared to other three groups. Viability was significantly
(p<0.05) higher in Groups II, III, and IV than Group I at the
pre-freeze stage. Significantly (p<0.01) higher viability and
acrosomal integrity were recorded in Group III as compared to
other three groups at the post-thaw stage. Abnormality was
significantly (p<0.05) higher in Group IV than other three
groups. HOST response was significantly (p<0.05) higher in
Groups II and III than Groups I and IV at the pre-freeze and
post-thaw stages.
Conclusion:
Addition of egg yolk powder at 4% level yielded significantly
better results in terms of post-thaw semen quality as compared
to the fresh egg yolk and other concentrations of egg yolk
powder (2% and 6%).
Keywords:
buffalo semen, egg yolk powder, freezability.
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