Open Access
Research
(Published
online: 21-06-2016)
15.
Characterization of promoter sequence of
toll-like receptor genes in Vechur cattle -
R. Lakshmi, K. K. Jayavardhanan and T. V. Aravindakshan
Veterinary World, 9(6): 626-632
doi:
10.14202/vetworld.2016.626-632
R. Lakshmi:
Department of Veterinary Biochemistry, College of Veterinary and
Animal Sciences, Thrissur, Kerala, India; lakshmivetbio@gmail.com
K. K. Jayavardhanan:
Department of Veterinary Biochemistry, College of Veterinary and
Animal Sciences, Thrissur, Kerala, India; jayavardhanan@kvasu.ac.in
T. V. Aravindakshan:
Centre for Advanced Studies in Animal Genetics and Breeding,
College of Veterinary and Animal Sciences, Thrissur, Kerala,
India; aravindakshantv@kvasu.ac.in
Received: 29-02-2016, Accepted: 18-05-2016, Published online:
21-06-2016
Corresponding author:
R. Lakshmi, e-mail: lakshmivetbio@gmail.com
Citation:
Lakshmi R, Jayavardhanan KK, Aravindakshan TV (2016)
Characterization of promoter sequence of toll-like receptor
genes in Vechur cattle,
Veterinary World, 9(6):
626-632.
Abstract
Aim:
To analyze the promoter sequence of toll-like receptor (TLR)
genes in Vechur cattle, an indigenous breed of Kerala with the
sequence of
Bos taurus
and access the differences that could be attributed to innate
immune responses against bovine mastitis.
Materials and Methods:
Blood samples were collected from Jugular vein of Vechur cattle,
maintained at Vechur cattle conservation center of Kerala
Veterinary and Animal Sciences University, using an
acid-citrate-dextrose anticoagulant. The genomic DNA was
extracted, and polymerase chain reaction was carried out to
amplify the promoter region of TLRs. The amplified product of
TLR2, 4, and 9 promoter regions was sequenced by Sanger
enzymatic DNA sequencing technique.
Results:
The sequence of promoter region of TLR2 of Vechur cattle with
the
B. taurus
sequence present in GenBank showed 98% similarity and revealed
variants for four sequence motifs. The sequence of the promoter
region of TLR4 of Vechur cattle revealed 99% similarity with
that of
B. taurus
sequence but not reveals significant variant in motifregions.
However, two heterozygous loci were observed from the
chromatogram. Promoter sequence of TLR9 gene also showed 99%
similarity to
B. taurus
sequence and revealed variants for four sequence motifs.
Conclusion:
The results of this study indicate that significant variation in
the promoter of TLR2 and 9 genes in Vechur cattle breed and may
potentially link the influence the innate immunity response
against mastitis diseases.
Keywords:
mastitis, promoter, sequence, toll-like receptor, Vechur breed.
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