Open Access
Research
(Published
online: 23-06-2016)
17.
Immunotoxicological, biochemical, and
histopathological studies on Roundup and Stomp herbicides in
Nile catfish (Clarias gariepinus) -
Gihan G. Moustafa, F. E. Shaaban, A. H. Abo
Hadeed, and Walaa M. Elhady
Veterinary World, 9(6): 638-647
doi:
10.14202/vetworld.2016.638-647
Gihan G. Moustafa:
Department of
Forensic Medicine and Toxicology, Faculty of Veterinary
Medicine, Zagazig University, Alzeraa Street Postal Code 44511,
Zagazig City, Sharkia Province, Egypt; gihan292@hotmail.com
F. E. Shaaban:
Department of
Forensic Medicine and Toxicology, Faculty of Veterinary
Medicine, Zagazig University, Alzeraa Street Postal Code 44511,
Zagazig City, Sharkia Province, Egypt; mahmoudnasr292@hotmail.com
A. H. Abo Hadeed:
Department of
Forensic Medicine and Toxicology, Faculty of Veterinary
Medicine, Zagazig University, Alzeraa Street Postal Code 44511,
Zagazig City, Sharkia Province, Egypt; alidido@hotmail.com
Walaa M. Elhady:
Department
of Forensic Medicine and Toxicology, Faculty of Veterinary
Medicine, Zagazig University, Alzeraa Street Postal Code 44511,
Zagazig City, Sharkia Province, Egypt; drwalaaelhady@yahoo.com
Received: 10-02-2016, Accepted: 20-05-2016, Published online:
23-06-2016
Corresponding author:
Gihan G.
Moustafa, e-mail: gihan292@hotmail.com
Citation:
Moustafa GG, Shaaban FE, Hadeed AHA, Elhady WM (2016)
Immunotoxicological, biochemical, and histopathological studies
on Roundup and Stomp herbicides in Nile catfish (Clarias
gariepinus), Veterinary World, 9(6): 638-647.
Abstract
Aim:
The current
study was directed to investigate the immunotoxic and oxidative
stress effects of Roundup and Stomp herbicides and their
combination on Nile catfish (Clarias gariepinus).
Materials and
Methods:
The experiment
was carried out on 120 fish that randomly divided into four
equal groups with three replicates: The first group kept as
control, the second group exposed to 1/2 96 h lethal
concentration 50 (LC50)
of Roundup, the third group exposed to 1/2 96 h LC50
of Stomp, and the fourth one exposed to a
combination of Roundup and Stomp at previously-mentioned doses.
The experiment was terminated after 15 days; blood samples were
obtained at 1st,
8th,
and 15th
days of treatment where the sera were separated
for estimation of antioxidant enzymes. Meanwhile, at 15th
day of exposure part of blood was collected from
all groups with an anticoagulant for evaluation of phagocytic
activity, then the fish were sacrificed, and specimens from the
liver of all groups were obtained for histopathological
examination.
Results:
Our
results indicated that both herbicides either individually or in
combination elucidated significant decrease in phagocytic
activity that was highly marked in group exposed to both
herbicides. Furthermore, our data elicited an obvious elevation
in the levels of superoxide dismutase (SOD), catalase (CAT), and
glutathione peroxidase (GPx). Meanwhile, the data depicted
reduction in levels of reduced glutathione (GSH) and
glutathione-S-transferase (GST). Histopathological investigation
of liver proved the aforementioned results.
Conclusion:
It
could be concluded that either Roundup or Stomp alone cause
significant deleterious effects on aquatic vertebrates. However,
the use of their combination enhanced their toxic effects.
Toxicity can end up in humans through the food chain.
Keywords:
antioxidant enzymes, fish, phagocytosis, Roundup, Stomp.
References
1. Diez, M.C. (2010) Biological aspects involved in the
degradation of organic pollutants. J. Soil Sci. Plant Nutr.,
10: 244-267.
http://dx.doi.org/10.4067/s0718-95162010000100004 |
|
|
2. Glusczak, L., Loro, V.L., Pretto, A., Moraes, B.S., Raabe,
A., Duarte, M.F., da Fonseca, M.B., de Menezes, C.C. and
Valladao, D.M.D. (2011) Acute exposure to glyphosate
herbicide affects oxidative parameters in Piava (Leporinus
obtusidens). Arch. Environ. Contam. Toxicol., 61: 624-630.
http://dx.doi.org/10.1007/s00244-011-9652-4
PMid:21465245 |
|
|
3. Rossi, S.C., da Silva, M.D., Piancini, L.D.S., Ribeiro,
C.A.O., Cestari, M.M. and de Assis, H.C.S. (2011) Sublethal
effects of waterborne herbicides in tropical freshwater fish.
Bull. Environ. Contam. Toxicol., 87: 603-607.
http://dx.doi.org/10.1007/s00128-011-0397-6
PMid:21984662 |
|
|
4. Stara, A., Kristan, J., Zuskova, E. and Velisek, J.
(2013) Effect of chronic exposure to prometryne on oxidative
stress and antioxidant response in common carp (Cyprinus
carpio L.). Pestic. Biochem. Physiol., 105: 18-23.
http://dx.doi.org/10.1016/j.pestbp.2012.11.002
PMid:24238285 |
|
|
5. Wagenhoff, A., Townsend, C.R., Phillips, N. and Matthaei,
C.D. (2011) Subsidy-stress and multiple-stressor effects
along gradients of deposited fine sediment and dissolved
nutrients in a regional set of streams and rivers. Freshw.
Biol., 56: 1916-1936.
http://dx.doi.org/10.1111/j.1365-2427.2011.02619.x |
|
|
6. Lushchak, O.V., Kubrak, O.I., Storey, J.M., Storey, K.B.
and Lushchak, O.I. (2009) Low toxic herbicide Roundup
induces mild oxidative stress in goldfish tissues.
Chemosphere, 76: 932-937.
http://dx.doi.org/10.1016/j.chemosphere.2009.04.045
PMid:19450865 |
|
|
|
7. EPA, (Environmental Protection Agency). (2011) Pesticides
industry sales and usage: 2006 and 2007 market estimates.
In: Grube, A., Donaldson, D., Kiely, T. and Wu, L., editors.
Biological and Economic Analysis Division, Office of
Pesticide Programs, Office of Chemical Safety and Pollution
Prevention. U.S. Environmental Protection Agency,
Washington, DC, USA. |
|
|
8. Romero, D.M., de Molina, M.C.R. and Juarez, A.B. (2011)
Oxidative stress induced by a commercial glyphosate
formulation in a tolerant strain of Chlorella kessleri.
Ecotoxicol. Environ. Saf., 74: 741-747.
http://dx.doi.org/10.1016/j.ecoenv.2010.10.034
PMid:21074853 |
|
|
9. Jiraungkoorskul, W., Upatham, E.S., Kruatrachue, M.,
Sahaphong, S., Vichasri-Grams, S. and Pokethitiyook, P.
(2002) Histopathological effects of Roundup, a glyphosate
herbicide, on Nile tilapia (Oreochromis niloticus).
ScienceAsia, 28: 121-127.
http://dx.doi.org/10.2306/scienceasia1513-1874.2002.28.121 |
|
|
|
10. Fetvadjieva, N., Straka, F., Michailova, P., Balinov,
I., Lubenov, I., Balinova, A., Pelov, V., Karsova, V. and
Tsvetkov, D. (1994) In: Fetvadjieva, N., editor. Handbook of
Pesticides. 2nd Revised. Zemizdat Inc., Sofia. p330. |
|
|
|
11. Meister, R.T., editor. (1992) Farm Chemicals Handbook
'92. Meister Publishing Company, Willoughby, Ohio, USA.
p197-202. |
|
|
12. Lushchak, V.I. (2011) Environmentally induced oxidative
stress in aquatic animals. Aquat. Toxicol., 101: 13-30.
http://dx.doi.org/10.1016/j.aquatox.2010.10.006
PMid:21074869 |
|
|
|
13. Perez, J., Domingues, I., Monteiro, M., Soares, A.M. and
Loureiro, S. (3013) Synergistic effects caused by atrazine
and terbuthylazine on chlorpyrifos toxicity to early - Life
stages of the zebrafish Danio rario. Environ. Sci. Pollut.
Res., 20: 4671-4680. |
|
|
14. Dabas, A., Nagpure, N.S., Kumar, R., Kushwaha, B.,
Kumar, P. and Lakra W. (2012) Assemment of tissue-specific
effect of cadmium on antioxidant defense system and lipid
peroxidation in freshwater murrel, Channa punctatus. Fish
Physiol. Biochem., 38: 468-482.
http://dx.doi.org/10.1007/s10695-011-9527-7
PMid:21691727 |
|
|
15. Abdelghani, A.A., Tchounwou, P.B., Anderson, A.C.,
Sujono, H., Heyer, L.R. and Monkiedje, A. (1997) Toxicity
evaluation of single and chemical mixtures of Roundup,
Garlon-3A, 2,4-D, and synthetic detergent surfactant to
channel catfish (Ictalurus punctatus), bluegill sunfish (Lepomis
microchirus), and crawfish (Procambarus spp.). Environ.
Toxicol. Water, 12: 237-243.
http://dx.doi.org/10.1002/(SICI)1098-2256(1997)12:3<237::AID-TOX6>3.0.CO;2-9 |
|
|
|
16. Kidd, H. and James, D.R. (1991) The Agrochemicals
Handbook. 3rd ed. Royal Society of Chemistry Information
Services, Cambridge, UK. p3-11. |
|
|
|
17. USEPA, (United State Environmental Protection Agency).
(1987) Pesticide Tolerance for Pendimethalin. Fed. Regist.
52: 47734.5. Dec. 16-1987. USEPA, Washington, DC, USA.
p10-117. |
|
|
|
18. Lucky, Z. (1977) Methods for the Diagnosis of Fish
Diseases. 1st ed. Amerind Publishing Co., Pvt., Ltd., New
Delhi, Bombay, Calcutta and New York. p140. |
|
|
|
19. Cruickshank, R., Duguid, R., Marmion, B.P. and Swain,
R.A. (1975) Natural and acquired immunity in: Medical
microbiology. In: Cruickshank, R. and Swain, R.A., editors.
Microbiology; Infection and Immunology. Vol. 1. Part 1.
Churchill Livingstone, Edinburgh and London. p137. |
|
|
|
20. Wilkinson, P.C. (1977) In: Thompson, R.A., editor.
Techniques in Clinical Immunology. Oxford Blackwell
Publication, USA. p201-212. |
|
|
|
21. Lucy, F.I. and Larry, D.B. (1982) Ontageny and line
differences in mitogenic responces of chicken lymphocyte.
Poult. Sci., 62: 579-584. |
|
|
|
22. Misra, H. and Fridivich, I. (1972) Role of superoxide
anion in autooxidation of epinephrine, simple assay for SOD.
J. Biochem., 247: 3170-3175. |
|
|
23. Packer, L. and Glazer, A.N. (1990) Method in enzymology.
Oxygen Radicals in Biological Systems, Part B; Oxygen
Radicals and Antioxidants. Vol. 186. Academic Press Inc.,
New York. p355-367.
http://dx.doi.org/10.1016/0076-6879(90)86129-J |
|
|
24. Sinha, A. K. (1972) Colorimetric assay of catalase.
Analytical Biochemistry, 47, 389 p251.
http://dx.doi.org/10.1016/0003-2697(72)90132-7 |
|
|
25. Beutler, E. (1957) Glutathione instability of drug -
Sensitive red cells; A new method for the in vitro detection
of drug sensitivity. J. Lab. Clin. Med., 49: 84-95.
PMid:13385579 |
|
|
|
26. Beutler, E., Duron, O. and Kelly B.M. (1963) Improved
method for the determination of blood glutathione. J. Lab.
Clin. Med., 61: 822-888. |
|
|
27. Habig, W.H., Pabst, M.J. and Jacoby, W.B. (1974)
Glutathione S-transferases. The first enzymatic step in
mercapturic acid formation. J. Biol. Chem. 249: 7130-7139.
PMid:4436300 |
|
|
28. Puertas, M.C., Martos, J.M.M., Cobo, M.P., Carrera, M.D.
and Exposito, M.J.R. (2012) Plasma oxidative stress
parameters in men and women with early stage Alzheimer type
dementia. Exp. Gerontol., 47: 625-630.
http://dx.doi.org/10.1016/j.exger.2012.05.019
PMid:22664577 |
|
|
|
29. Suvarna, S.K., Layton, C. and Bancroft, J.D. (2013)
Bancroft's Theory and Practice of Histological Techniques.
7th ed. Churchill Livingstone, Elsevier, England. |
|
|
30. Sandrini, J.Z., Rola, R.C., Lopes, F.M., Buffon, H.F.,
Freitas, M.M., Martins, C.M.G. and Rosa, C.E. (2013) Effects
of glyphosate on cholinesterase activity of the mussel
Pernaperna and the fish Danio rerio and Jenynsia
multidentata: In vitro studies. Aquat. Toxicol., 130:
171-173.
http://dx.doi.org/10.1016/j.aquatox.2013.01.006
PMid:23411353 |
|
|
31. Betoulle, S., Duchiron, C. and Deschaux, P. (2000)
Lindane differently modulates intracellular calcium levels
in two populations of rainbow trout (Oncorhynchus mykiss)
immune cells: Head kidney phagocytes and peripheral blood
leucocytes. Toxicology, 145: 203-215.
http://dx.doi.org/10.1016/S0300-483X(99)00226-7 |
|
|
32. Harikrishnan, R., Balasundaram, C., Kim, M.C., Kim, J.S.,
Han, Y.J. and Heo, M.S. (2009) Innate immune response and
disease resistance in Carassius auratus by triherbal solvent
extracts. Fish Shellfish Immunol., 27: 508-515.
http://dx.doi.org/10.1016/j.fsi.2009.07.004
PMid:19616631 |
|
|
33. Galloway, T.S. and Depledge, M.H. (2001) Immunotoxicity
in invertebrates, measurement and ecotoxicological
relevance. Ecotoxicology, 10: 5-23.
http://dx.doi.org/10.1023/A:1008939520263
PMid:11227817 |
|
|
34. Kreutz, L.C., Barcellos, L.J.G., Valle, S.F., Silva, T.O.,
Anziliero, D., Santos, E.D., Pivato, M. and Zanatta, R.
(2011) Altered hematological and immunological parameters in
silver catfish (Rhamdia quelen) following short term exposure
to sublethal concentration of glyphosate. Fish Shellfish
Immunol., 30: 51-57.
http://dx.doi.org/10.1016/j.fsi.2010.09.012
PMid:20883798 |
|
|
35. Danion, M., Le Floch, S., Kanan, R., Lamour, F. and
Quentel, C. (2012) Effects of in vivo chronic exposure to
pendimethalin/Prowl 400® on sanitary status and the immune
system in rainbow trout (Oncorhynchus mykiss). Sci. Total
Environ., 424: 143-152.
http://dx.doi.org/10.1016/j.scitotenv.2012.02.055
PMid:22444063 |
|
|
36. Camus, L., Jones, M., Břrseth, J., Grřsvik, B., Regoli,
F. and Depledge, M. (2002) Total oxyradical scavenging
capacity and cell membrane stability of haemocytes of the
Arctic scallop, Chlamys islandicus, following benzo(a)pyrene
exposure. Mar. Environ. Res., 54: 425-430.
http://dx.doi.org/10.1016/S0141-1136(02)00140-X |
|
|
37. Mohamed, A.H. (2011) Sublethal toxicity of Roundup to
immunological and molecular aspects of Biomphalaria
alexandrina to Schistosoma mansoni infection. Ecotoxicol.
Environ. Saf., 74: 754-760.
http://dx.doi.org/10.1016/j.ecoenv.2010.10.037
PMid:21126764 |
|
|
38. Matricon-Gondran, M. and Letocart, M. (1999) Internal
defenses of the snail Biompha-laria glabrata I.
Characterization of hemocytes and fixed phagocytosis. J.
Invertebr. Pathol., 74: 224-234.
http://dx.doi.org/10.1006/jipa.1999.4876
PMid:10534409 |
|
|
39. Ortiz-Ordonez, E., Uria-Galicia, E., Ruiz-Picos, R.A.,
Duran, A.G.S., Trejo, Y.H., Sedeno-Diaz, J.E. and
Lopez-Lopez, E. (2011) Effect of Yerbimat herbicide on lipid
peroxidation, catalase activity, and histological damage in
gills and liver of the freshwater fish Goodea atripinnis.
Arch. Environ. Contam. Toxicol., 61: 443-452.
http://dx.doi.org/10.1007/s00244-011-9648-0
PMid:21305274 |
|
|
40. Blahová, J., Plhalová, L., Hostovský, M., Divišov, L.,
Dobšíková, R., Mikulíková, I., Štĕpánová, S. and Svobodová,
Z. (2013) Oxidative stress responses in zebrafish Danio rerio
after subchronic exposure to atrazine. Food Chem. Toxicol.,
61: 82-85.
http://dx.doi.org/10.1016/j.fct.2013.02.041
PMid:23499751 |
|
|
41. Regoli, F. and Giuliani, M.E. (2014) Oxidative pathways
of chemical toxicity and oxidative stress biomarkers in
marine organisms. Mar. Environ. Res., 93: 106-117.
http://dx.doi.org/10.1016/j.marenvres.2013.07.006
PMid:23942183 |
|
|
42. Monteiro, D.A., de Almeida, J.A., Rantin, F.T. and
Kalinin, A.L. (2006) Oxidative stress biomarkers in the
freshwater characid fish, Brycon cephalus, exposed to
organophosphorus insecticide Folisuper 600 (methyl
parathion). Comp. Biochem. Phys. C., 143: 141-149.
http://dx.doi.org/10.1016/j.cbpc.2006.01.004 |
|
|
43. Kohen, R. and Nyska, A. (2002) Oxidation of biological
systems: Oxidative stress phenomena, antioxidants, redox
reactions, and methods for their quantification. Toxicol.
Pathol., 30: 620-650.
http://dx.doi.org/10.1080/01926230290166724 |
|
|
44. Van der Oost, R., Beyer, J. and Vermeulen, N.P. (2003)
Fish bioaccumulation and biomarkers in environmental risk
assessment: A review. Environ. Toxicol. Pharmacol., 13:
57-149.
http://dx.doi.org/10.1016/S1382-6689(02)00126-6 |
|
|
45. Nwani, C.D., Nagpure, N.S., Kumar, R., Kushwaha, B. and
Lakra, W.S. (2013) DNA damage and oxidative stress
modulatory effects of glyphosate-based herbicide in
freshwater fish, Channa punctatus. Environ. Toxicol.
Pharmacol., 36: 539-547.
http://dx.doi.org/10.1016/j.etap.2013.06.001
PMid:23816461 |
|
|
46. John, S., Kale, M., Rathore, N. and Bhatnagar, D. (2001)
Protective effect of vitamin E in dimethoate and malathion
induced oxidative stress in rat erythrocytes. J. Nutr.
Biochem., 12: 500-504.
http://dx.doi.org/10.1016/S0955-2863(01)00160-7 |
|
|
47. Pieniazek, D., Bukowska, B. and Duda, W. (2004)
Comparison of the effect of Roundup Ultra 360 SL pesticide
and its active compound glyphosate on human erythrocytes.
Pestic. Biochem. Physiol., 79: 27-34.
http://dx.doi.org/10.1016/j.pestbp.2004.03.003 |
|
|
48. Noori, S. (2012) An overview of oxidative stress and
antioxidant defensive system. Scientific Reports,1:413.
http://dx.doi.org/10.4172/scientificreports.413 |
|
|
49. Yilmaz, S., Atessahin, A., Sahna, E., Karahan, I. and
Ozer, S. (2006) Protective effect of lycopene on adriamycin-induced
cardiotoxicity and nephrotoxicity. Toxicology, 218: 164-171.
http://dx.doi.org/10.1016/j.tox.2005.10.015
PMid:16325981 |
|
|
50. Bukowska, B. (2003) Effects of 2,4-d and its metabolite
2,4-dichlorophenol on antioxidant enzymes and level of
glutathione in human erythrocytes. Comp. Biochem. Physiol.
C., 135: 435-441.
http://dx.doi.org/10.1016/s1532-0456(03)00151-0 |
|
|
51. Danion, M., Le Floch, S., Lamour, F. and Quentel, C.
(2014) Effects of in vivo chronic exposure to pendimethalin
on EROD activity and antioxidant defenses in rainbow trout
(Oncorhynchus mykiss). Ecotoxicol. Environ. Saf., 99: 21-27.
http://dx.doi.org/10.1016/j.ecoenv.2013.09.024
PMid:24183287 |
|
|
52. Cereser, C., Boget, S., Parvaz, P. and Revol, A. (2001)
Thiram-induced cytotoxic-ity is accompanied by a rapid and
drastic oxidation of reduced glutathione with consecutive
lipid peroxidation and cell death. Toxicology, 163: 153-162.
http://dx.doi.org/10.1016/S0300-483X(01)00401-2 |
|
|
53. Banerjee, B.D., Seth, V., Bhattacharya, A., Pasha, S.T.
and Chakraborty, A.K. (1999) Biochemical effects of some
pesticides on lipid peroxidation and free-radical
scavengers. Toxicol. Lett., 107: 33-47.
http://dx.doi.org/10.1016/S0378-4274(99)00029-6 |
|
|
54. Slaughter, M.R., Thakkar, H. and O'Brien, P.J. (2002)
Effect of diquat on the antioxidant system and cell growth
in human neuroblastoma cells. Toxicol. Appl. Pharmacol.,
178: 63-70.
http://dx.doi.org/10.1006/taap.2001.9322
PMid:11814326 |
|
|
55. Tsukamoto, M., Tampo, Y., Sawada, M. and Yonaha, M.
(2002) Paraquat induced oxidative stress and dysfunction of
the glutathione redox cycle in pulmonary microvascular
endothelial cells. Toxicol. Appl. Pharmacol., 178: 82-92.
http://dx.doi.org/10.1006/taap.2001.9325
PMid:11814328 |
|
|
|
56. Machala, M., Petřivalský, M., Nezveda, K., Ulrico, R.,
Dušek, L., Piačka, V. and Svobodová, Z. (1997) Responses of
carp hepatopancreatic 7-ethoxyresorufin-O-deethylase and
glutathione-dependent enzymes to organic pollutants - A
field study. Environ. Toxicol. Chem., 16: 1410-1416. |
|
|
57. Bagnyukova, T.V., Chahrak, O.I. and Lushchak, V.I.
(2006) Coordinated response of goldfish antioxidant defenses
to environmental stress. Aquat. Toxicol., 78: 325-331.
http://dx.doi.org/10.1016/j.aquatox.2006.04.005
PMid:16735067 |
|
|
58. Hermes-Lima, M. and Storey, K.B. (1993) In vitro
oxidative inactivation of glutathione S-transferase from a
freeze tolerant reptile. Mol. Cell. Biochem., 124: 149-158.
http://dx.doi.org/10.1007/BF00929207
PMid:8232286 |
|
|
59. Modesto, K.A. and Martinez, C.B.R. (2010) Roundup causes
oxidative stress in liver and inhibits acetylcholinesterase
in muscle and brain of the fish Prochilodus lineatus.
Chemosphere, 78: 294-299.
http://dx.doi.org/10.1016/j.chemosphere.2009.10.047
PMid:19910015 |
|
|
60. Moreno, I., Pichardo, S., Góomez-Amores, L., Mate, A.,
Vazquez, C.M. and Cameán, A.M. (2005) Antioxidant enzyme
activity and lipid peroxidation in liver and kidney of rats
exposed to microcystin-LR administered intraperitoneally.
Toxicon, 45: 395-402.
http://dx.doi.org/10.1016/j.toxicon.2004.11.001
PMid:15733560 |
|
|
61. Gehin, A., Guyon, C. and Nicod, L. (2006) Glyphosate-induced
antioxidant imbalance in HaCaT: The protective effect of
vitamins C and E. Environ. Toxicol. Pharmacol., 22: 27-34.
http://dx.doi.org/10.1016/j.etap.2005.11.003
PMid:21783682 |
|
|
62. Maran, E., Fernández, M., Barbieri, P., Font, G. and
Ruiz, M.J. (2009) Effects of four carbamate compounds on
antioxidant parameters. Ecotoxicol. Environ. Saf., 72:
922-930.
http://dx.doi.org/10.1016/j.ecoenv.2008.01.018
PMid:18328561 |
|
|
63. Shiogiri, N.S., Paulino, M.G., Carraschi, S.P., Baraldi,
F.G., Cruz, C. and Fernandes, M.N. (2012) Acute exposure of
a glyphosate-based herbicide affects the gills and liver of
the neotropical fish, Piaractus mesopotamicus. Environ.
Toxicol. Pharmacol., 2: 388-496.
http://dx.doi.org/10.1016/j.etap.2012.05.007
PMid:22743578 |
|
|
64. Langiano, V.C. and Martinez, C.B. (2008) Toxicity and
effects of a glyphosate-based herbicide on the neotropical fish
Prochilodus lineatus. Comp. Biochem. Physiol. C., 147:
222-231.
http://dx.doi.org/10.1016/j.cbpc.2007.09.009 |
|
|
|
65. Abd-Algadir, M.I., Elkhier, M.K.S. and Idris, O.F.
(2011) Changes of fish liver (Tilapia nilotica) made by
herbicide (Pendimethalin). J. Appl. Biosci., 43: 2942-2946. |
|
|
|
66. Nabela, I.E., Reda, R.M. and El-Araby, I.E. (2011)
Assessment of Stomp® (Pendimethalin) toxicity on Oreochromis
niloticus. J. Am. Sci., 7: 568-576. |
|
