Open Access
Research
(Published
online: 29-05-2016)
18.
The structural and functional recovery of
pancreatic β-cells in type 1 diabetes mellitus induced
mesenchymal stem cell-conditioned medium -
Widagdo Sri Nugroho, Dwi Liliek Kusindarta, Heru Susetya, Ida
Fitriana, Guntari Titik Mulyani, Yuda Heru Fibrianto, Aris
Haryanto and Teguh Budipitojo
Veterinary World, 9(5): 535-539
doi:
10.14202/vetworld.2016.535-539
Widagdo Sri Nugroho:
Department of Veterinary Public Health, Faculty of Veterinary
Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;
weesnugroho@ugm.ac.id
Dwi Liliek Kusindarta:
Department of Anatomy, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia; indarta@ugm.ac.id
Heru Susetya:
Department of Veterinary Public Health, Faculty of Veterinary
Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;
heruanggikiki@yahoo.com
Ida Fitriana:
Department of Pharmacology, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia; idafitriana.apt@gmail.com
Guntari Titik Mulyani:
Department of Internal Medicine, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia; guntari@ugm.ac.id
Yuda Heru Fibrianto:
Department of Physiology, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia; fibrianto1802@gmail.com
Aris Haryanto:
Department of Biochemistry, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia; arisharyanto@yahoo.com
Teguh Budipitojo:
Department of Anatomy, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia; budipitojo@ugm.ac.id
Received: 02-11-2015, Accepted: 22-04-2016, Published online:
29-05-2016
Corresponding author:
Teguh Budipitojo, e-mail: budipitojo@ugm.ac.id
Citation:
Nugroho WS, Kusindarta DL, Susetya H, Fitriana I, Mulyani GT,
Fibrianto YH, Haryanto A, Budipitojo T (2016) The structural and
functional recovery of pancreatic β-cells in type 1 diabetes
mellitus induced mesenchymal stem cell-conditioned medium,
Veterinary World, 9(5):
535-539.
Abstract
Aim:
Various studies have shown that secreted factors alone in
culture medium without stem cell are capable of repairing
tissues by itself in various conditions involving damaged
tissue/organ. Therefore, this study was aimed to investigate the
role of human umbilical cord mesenchymal stem cell-derived
conditioned medium (CM) on the recovery of pancreatic β-cells in
Wistar rats (Rattus
norvegicus)
with type 1 diabetes mellitus.
Materials and Methods:
The 0.05 ml CM induction was applied to the diabetic group of
rats in weeks 1, 2, 3, and 4. 1 week after each CM induction,
insulin concentration was analyzed using ELISA. The pancreas was
divided into 3 regions, processed by paraffin method, stained
with hematoxylin-eosin, and immunohistochemical method for
insulin.
Results:
This study indicated the decrease in the total number of islets
and insulin concentration after the injection of single dose of
alloxan. The exocrine acini were also damaged. Microscopic
observation detected the presence of small islets in the
diabetic group 1 week after the first 0.05 ml CM induction. The
number and size of the islets increased in line with the CM
doses and time of inductions. Immunohistochemically, the
presence of low intensity of insulin-positive cells could be
recognized at the splenic and duodenal regions of the pancreas,
but not gastric region, 1 week after the first and second 0.05
ml CM induction. The intensity of staining and the number of
insulin-positive cells increased dramatically in 1 week after
the third and fourth 0.05 ml of CM induction in all regions of
the pancreas. The data of insulin blood concentration showed
clear differences between the second and the fourth induction of
0.05 ml CM induction.
Conclusions:
This study showed very strong evidence on the role of human
umbilical cord mesenchymal stem cell-derived CM in recovering
the pancreatic β-cells damage in Wistar rats (R.
norvegicus)
with type 1 diabetes mellitus, structurally and functionally.
Keywords:
conditioned-medium, pancreatic β-cells, structural and
functional recovery, type 1 diabetes mellitus.
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