Open Access
Research
(Published
online: 21-11-2016)
19.
Chronic exposure to indoxacarb and pulmonary
expression of toll-like receptor-9 in mice -
Sandeep Kaur, C. S. Mukhopadhyay and R. S. Sethi
Veterinary World, 9(11): 1282-1286
doi:
10.14202/vetworld.2016.1282-1286
Sandeep Kaur:
School of Animal Biotechnology, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana - 141 004, Punjab, India;
sndp4482@gmail.com
C. S. Mukhopadhyay:
School of Animal Biotechnology, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana - 141 004, Punjab, India;
csmbioinfo@gmail.com
R. S. Sethi:
School of Animal Biotechnology, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana - 141 004, Punjab, India;
rs.sethi@usask.ca
Received: 25-04-2016, Accepted: 14-10-2016, Published online:
21-11-2016
Corresponding author:
R. S. Sethi, e-mail: rs.sethi@usask.ca
Citation:
Kaur S, Mukhopadhyay CS, Sethi RS (2016) Chronic exposure to
indoxacarb and pulmonary expression of toll-like receptor-9 in
mice,
Veterinary World, 9(11):
1282-1286.
Abstract
Aim:
Chronic exposure to indoxacarb and pulmonary expression of
toll-like receptor 9 (TLR-9) in mice.
Materials and Methods:
In this study, healthy male Swiss albino mice (n=30) aging 8-10
weeks were used to evaluate TLR-9 expression in lungs of mice
following indoxacarb exposure with and without lipopolysaccharide
(LPS). Indoxacarb was administered orally dissolved in groundnut
oil at 4 and 2 mg/kg/day for 90 days. On day 91, five animals from
each group were challenged with LPS/normal saline solution at 80
μg/animal. The lung tissues were processed for real time and
immunohistochemical studies.
Results:
LPS resulted increase in fold change m-RNA expression level of
TLR-9 as compare to control, while indoxacarb (4 mg/kg) alone and
in combination with LPS resulted 16.21-fold change and 29.4-fold
change increase in expression of TLR-9 m-RNA, respectively, as
compared to control. Similarly, indoxacarb (2 mg/kg) alone or in
combination with LPS also altered TLR-9 expression. Further at
protein level control group showed minimal expression of TLR-9 in
lungs as compare to other groups, however, LPS group showed
intense positive staining in bronchial epithelium as well as in
alveolar septal cells. Indoxacarb at both doses individually
showed strong immuno-positive reaction as compare to control,
however when combined with LPS resulted intense staining in airway
epithelium as compare to control.
Conclusion:
Chronic oral administration of indoxacarb for 90 days (4 and 2
mg/kg) alters expression of TLR-9 at m-RNA and protein level and
co-exposure with LPS exhibited synergistic effect.
Keywords:
indoxacarb, lipopolysaccharide, lungs, mice, toll-like receptor-9.
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